Publications by authors named "Mizuha Shibata"
Development of a Novel Real-Time Polymerase Chain Reaction Assay to Detect in Chicken Meat.
Foodborne Pathog Dis
December 2022
Article Synopsis
- - An emerging enteropathogen has been linked to several foodborne outbreaks in Japan, but most foods associated with these outbreaks remain unidentified, highlighting the need for accurate detection methods.
- - The study developed a real-time PCR assay (EA-rtPCR) specifically targeting a gene of the pathogen, demonstrating its effectiveness with a detection limit of 2.0-3.2 log CFU/mL in enriched chicken samples.
- - Comparative testing showed that EA-rtPCR was more sensitive than nested-PCR in detecting the pathogen in chicken meat, with multiple positive findings in retail chicken and carcasses, indicating its potential to enhance food safety and research on infections.