Publications by authors named "Miyyada Boumechache"
Article Synopsis
- P2X7 receptors are ATP-gated cation channels that work with other proteins to trigger various cell-specific responses, but their role in cell death and sensitivity to NAD is not well understood.
- The P2X7k variant of the receptor, found in T lymphocytes, enhances sensitivity to NAD due to structural differences, improving its stability and function compared to the more common P2X7a variant.
- The interaction between P2X7 receptors and pannexin-1 is complex, with P2X7k showing independent activity in ethidium uptake, while signaling occurs downstream of caspase activation rather than direct interaction between the receptors.
P2X4 and P2X7 are the predominant P2X receptor subtypes expressed in immune cells. Having previously shown a structural and functional interaction between the two recombinant receptors, our aims here were to identify the preferred assembly pathway of the endogenous receptors in macrophage-like cells and to investigate the trafficking of these receptors between the plasma membrane and intracellular sites. We exploited the difference in size between the two subunits, and we used a combination of cross-linkers and blue native-PAGE analysis to investigate the subunit composition of complexes present in primary cultures of rat microglia and macrophages from wild type and P2X7(-/-) mice.
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