Monoclonal antibodies against human oral squamous cell carcinoma reacting with keratin proteins.

Two mouse monoclonal antibodies (MoAbs), B10 and 1H5, were generated by fusing mouse myeloma NS-1 cells with spleen cells from a BALB/c mouse immunized with Ueda-1 cells derived from human squamous cell carcinoma (SCC) of the floor of the mouth. Immunohistochemical analysis revealed that these MoAbs recognize the filamentous components of cytoplasm which were protein in nature. While the pattern of antigen distribution in various cell lines was not cell-type specific, reactivity of these antibodies with tissue sections was informative.

Effect of a diffusional barrier to a metabolite across hepatocytes on its kinetics in "enzyme-distributed" models: a computer-aided simulation study.

The effect of a diffusional barrier to a metabolite between the blood and hepatocytes on elimination kinetics of formed and preformed metabolites was predicted under various enzymic distributions in the liver by computer-aided simulation. Sequential metabolism by which the primary metabolite (MI) is generated from the parent drug (D) and further metabolized to the terminal metabolite (MII) by enzymes A and B, respectively, was chosen for the simulation. Moreover, four models of enzyme distribution patterns were defined with regard to the hepatic blood flow path.

Epidermal Langerhans cells undergo mitosis during the early recovery phase after ultraviolet-B irradiation.

We studied the recovery phase of immune response-associated (Ia)-positive or ATPase-positive epidermal Langerhans cells (ELCs) after ultraviolet B (UVB)-induced depletion by using mouse ear epidermal sheets. An area 3 mm in diameter was irradiated with 300 nm UVB light (40 mJ/cm2). A time sequence study was carried out to 56 days.

[Usefulness of caerulein in suppressing post-TAE complications of the gallbladder].

While transcatheter hepatic arterial embolization (TAE) has been extensively performed as a form of treatment for nonresectable malignant hepatic tumors, complications, such as abdominal pain, fever or leukocytosis due to gallbladder infarction by embolic materials frequently occur and have not yet been overcome. We devised a new procedure for reducing the incidence of gallbladder infarction by administering caerulein prior to TAE. Between 1984 and 1986, 63 patients with hepatocellular carcinoma were treated by TAE with the use of Gelfoam.

Electrophysiological evidence for dreaming: human cerebral potentials associated with rapid eye movement during REM sleep.

In order to examine the relationship between rapid eye movement (REM) during REM sleep and dreaming, scalp EEGs (Fz, Cz, Pz and Oz) of 4 normal human subjects, time-locked to REM onset, to saccade onset toward fixation targets and to saccade onset in total darkness, were averaged. The results include the following: Three positive potentials were associated with REM: a sharp potential in the parieto-occipital area just before REM onset; a large, slow potential in the vertex area 140-180 msec after REM onset; and a potential in the occipital area 210-280 msec after REM onset. Three positive potentials, one being the so-called EM-antecedent potential and the others being the lambda response, were associated with the waking saccades toward targets: a sharp potential in the parieto-occipital area just before the saccade onset and two potentials in the occipital area with latencies of 140-150 and 260-310 msec from the saccade onset.

Kinetics of hepatic transport of 4-methylumbelliferone in rats. Analysis by multiple indicator dilution method.

Hepatic elimination of 4-methylumbelliferone (4-MU), which has been used as a model compound for conjugative metabolism, was studied by means of a multiple indicator dilution (MID) method in the isolated perfused rat liver. Using this method, three intrinsic hepatic clearances, CLint,inf, CLint,eff, and CLint,seq, which represent the influx, efflux, and sequestration processes, respectively, were obtained. When the dose was increased from a low dose (50 micrograms/rat liver) to a high dose (3000 micrograms/rat liver), the hepatic availability of 4-MU increased from 0.

[Thymidylate synthase inhibition in malignant tumors after oral administration of UFT].

Surgical resection of solid tumors was undertaken five to six hours after the oral administration of UFT (300-500 mg/body) in five patients, who were (then) assayed for 5-fluorodeoxyuridylate (FdUMP), total dTMP synthase (TS) and non-FdUMP-bound, free enzyme. Three patients had gastric cancer, and two had mammary cancer. Control solid tumors were obtained without, the drug in eight patients.

A simulation study on the effect of a uniform diffusional barrier across hepatocytes on drug metabolism by evenly or unevenly distributed uni-enzyme in the liver.

The effect of a uniform diffusional barrier on hepatic extraction of the parent drug by evenly or unevenly distributed uni-enzyme was quantitatively determined by the present simulation study. Five models of enzymic distribution were defined with regard to the hepatic blood flow path, and the extraction ratios were calculated or simulated under the various conditions of average intrinsic clearances and diffusion clearances across hepatocytes. Differences in the extraction ratios among the five models were evaluated by the "relative extraction ratios," which are the extraction ratios in each model divided by that in the model where the enzymatic activity is evenly distributed.

Evaluations on the usefulness of viscous agents in anterior segment surgery. I. The ability to maintain the deepness of the anterior chamber.

Maintenance of the deepness of the anterior chamber is necessary to prevent the direct contact of surgical instruments with the corneal endothelium. Therefore, in this study, we measured the ability of viscous agents to maintain the deepness of the anterior chamber in vitro and discussed the correlations between this ability and the viscosity or elasticity of the various viscous agents. A 1% solution of sodium hyaluronate with a molecular weight of 2160 X 10(3) which has the highest viscosity and elasticity showed the best ability to maintain deepness.