The rapid discovery of highly active butyrylcholinesterase (BChE) inhibitors is key to the treatment of the late stages of Alzheimer's disease. Herein, a colorimetric cellulose membrane (CM)-based biosensor was developed. The CM was employed as a carrier, which can be immobilized with the BChE and 5,5'-dithio-(2-nitrobenzoic acid) (DTNB) to prepare the biosensor for the solid-phase enzyme-catalyzed reaction.
View Article and Find Full Text PDFUric acid (UA) serves as an important biochemical marker of various diseases, making the development of a novel method for its rapid and straightforward visual detection highly valuable. In this study, a uricase-based cellulose membrane biosensor (UCMB) was constructed by immobilizing uricase via a Schiff base reaction and nitroblue tetrazolium chloride (NBT) through adsorption. The UCMB detects UA through a mechanism in which uricase catalyzes the oxidation of UA, generation O radicals that subsequently oxidize NBT to formazan, producing a distinctive color change from yellow to purple.
View Article and Find Full Text PDFExploring non-noble and high-performance metal catalysts to replace platinum-based catalysts for the hydrogen evolution reaction (HER) electrochemical water splitting significantly alleviates environmental pollution and the energy crisis. However, the synthetic approaches of such electrocatalysts are generally complex and challenging for large-scale production. Herein, a facile and green solid-state synthesis of Ni nanoparticles decorated with N-doped porous carbon is presented.
View Article and Find Full Text PDFXanthine oxidase (XO) is a typical target for hyperuricemia and gout, for which there are only three commercial xanthine oxidase inhibitors (XOIs): febuxostat, topiroxostat and allopurinol. However, these inhibitors have problems such as low bioactivity and several side effects. Therefore, the development of novel XOIs with high bioactivity for the treatment of hyperuricemia and gout is urgently needed.
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