Interactive histone acetylation and methylation in regulating transdifferentiation-related genes during tunicate budding and regeneration.

Background: In the budding tunicate Polyandrocarpa misakiensis, retinoic acid (RA)-triggered transdifferentiation occurs during bud development and zooid regeneration. We aimed to reveal how and to what extent epigenetic histone modifications are involved in transdifferentiation-related gene expression.

Results: Acetylated histone H3 lysine 9 (H3K9ac) was observed in transdifferentiating bud tissues and regenerating zooid tissues, where a histone acetyltransferase (HAT) gene, PmGCN5, was strongly expressed.