Complete Genome Sequences of Ralstonia solanacearum Strains Isolated from Zingiberaceae Plants in Japan.

Here, we report the complete genome sequences of three strains isolated from Zingiberaceae plants in Japan. The total genome sizes of these strains ranged from 5.87 to 6.

On-farm solid state simultaneous saccharification and fermentation of whole crop forage rice in wrapped round bale for ethanol production.

Background: In an attempt to reduce environmental loading during ethanol production from cellulosic plant biomass, we have previously proposed an on-site solid state fermentation (SSF) method for producing ethanol from whole crops, which at the same time provides cattle feed without producing wastes. During the ensiling of freshly harvested plant biomass with cellulase and glucoamylase, the added yeast and lactic acid bacteria induced simultaneous saccharification and production of ethanol and lactic acid in hermetically sealed containers on-farm. In a previous study, laboratory-scale SSF (using 250 g of fresh rice crop biomass) yielded 16.

Silage produces biofuel for local consumption.

Background: In the normal process of bioethanol production, biomass is transported to integrated large factories for degradation to sugar, fermentation, and recovery of ethanol by distillation. Biomass nutrient loss occurs during preservation and degradation. Our aim was to develop a decentralized ethanol production system appropriate for farm or co-operative level production that uses a solid-state fermentation method for producing bio-ethanol from whole crops, provides cattle feed, and produces no wastes.

DNA Analysis of Ralstonia solanacearum and Related Bacteria Based on 282-bp PCR-Amplified Fragment.

Strains of Ralstonia solanacearum, Pseudomonas syzygii, and the blood disease bacterium (BDB) from different countries were tested for polymerase chain reaction amplification of the 282-bp fragment using the primer pair 759 and 760. These 282-bp fragments from 49 strains of R. solanacearum, six strains of P.