Detection of respiratory viruses in nasopharyngeal secretions and middle ear fluid from children with acute otitis media.

Conclusions: Our results suggest that various respiratory viruses contribute to the pathogenesis of acute otitis media (AOM).

Objective: AOM is one of the most common complications of viral upper respiratory tract infections in children. Recently, the importance of respiratory viruses has been stressed as causative agents of AOM.

Acute otitis media associated with cytomegalovirus infection in infants and children.

Objective: Acute otitis media (AOM) is one of the most common complications of viral respiratory tract infections in children, but the role of each virus is still to be elucidated. We analyzed AOM associated with infection by cytomegalovirus (CMV), which is known as one of the major causes of viral respiratory tract infection.

Methods: Four hundred and ninety-five children (292 boys and 203 girls) diagnosed as having AOM in 2002 were studied.

Audiometry with nasally presented masking noise: novel diagnostic method for patulous eustachian tube.

Objective: Nasal-noise masking audiometry was developed to assess the acoustic transfer function from the nasopharyngeal cavity to the middle ear via patulous eustachian tube (ET).

Study Design: Prospective.

Setting: Tertiary referral center.

Trans-tympanic silicone plug insertion for chronic patulous Eustachian tube.

Conclusion: Trans-tympanic insertion of a new silicone plug seems to be useful for controlling the distressing symptoms of patients with a chronic patulous Eustachian tube (PET).

Objective: To evaluate the effectiveness of a new silicone plug for blocking the isthmus of a PET in patients whose symptoms were resistant to other therapies for > 6 months.

Material And Methods: The silicone plug (total length 23-25 mm; tip diameter 1.

Isolation of measles virus from middle ear fluid of infants with acute otitis media.

Measles virus was isolated from the middle ear fluid (MEF) of two infant cases of acute otitis media (AOM) associated with measles. This is the first report on the isolation of measles virus from the MEF in patients with AOM, and possibility of the measles virus as a causative agent of AOM was suggested.

Detection of human metapneumovirus from children with acute otitis media.

Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.

Relationship between respiratory syncytial virus infection and acute otitis media in children.

Background: Respiratory syncytial virus (RSV) is among the major causes of respiratory tract infection in infants and young children, and concomitant acute otitis media (AOM) often develops. However, there are only a few reports about AOM associated with RSV infection.

Methods: Two hundred and thirty children who were diagnosed as having RSV infection were studied by enzyme immunoassay (Testpack RSV) at the Department of Pediatrics of Tohoku Rosai Hospital from 1 November 2001 to 31 October 2002.