BAL1 and BBAP are regulated by a gamma interferon-responsive bidirectional promoter and are overexpressed in diffuse large B-cell lymphomas with a prominent inflammatory infiltrate.

BAL1 is a transcription modulator that is overexpressed in chemoresistant, diffuse large B-cell lymphomas (DLBCLs). BAL1 complexes with a recently described DELTEX family member termed BBAP. Herein, we characterized BAL1 and BBAP expression in primary DLBCL subtypes defined by their comprehensive transcriptional profiles.

Dual regulation of the anaphase promoting complex in human cells by cyclin A-Cdk2 and cyclin A-Cdk1 complexes.

In mammalian somatic cells, the Anaphase Promoting Complex (APC) is inactivated during S phase by active cyclin A-Cyclin dependent kinase (Cdk) 2 complexes promoting accumulation of mitotic regulators, such as cyclin B and Polo like kinase 1 (Plk1). However, mitotic entry does not appear to be perturbed in some human cancer cells or in normal mouse cells following Cdk2 RNA interference (i) or deletion of the Cdk2 gene. These results suggest functional complementation of APC regulation by a compensatory kinase.

Utilisation pattern of blood in a teaching hospital of Kolkata.

An annual utilisation of blood transfusion services at several depts. of R.G.

Lipoblastomatosis--a case report.

Lipoblastoma and lipoblastomatosis are benign fatty tumors of soft tissues that are unique to childhood. A male infant aged 5 months presented with a history of recurrent lump in the left scapular region. He had been operated upon for the same lump 2 months earlier.

Cyclin A/Cdk2 complexes regulate activation of Cdk1 and Cdc25 phosphatases in human cells.

Mitotic entry, a critical decision point for maintaining genetic stability, is governed by the cyclin B/Cyclin dependent kinase 1 (Cdc2) complex. In Xenopus oocytes and early embryos, accumulation of cyclin B activates Cdk1, which then phosphorylates and activates the positive regulator Cdc25 in an autocatalytic feedback loop. However, cyclin B levels do not increase as some human cells approach mitosis, and the key factors regulating Cdk1 activation in human cells are unknown.

The geographical data of the Kāśyapa Samhitā.

One of the parameters in ascertaining the date of the Kashyapa Samhita is the data of geography referred to there in. Geography abounds various spheres like country, province, city, river, mountain and sea. The whole geographical material is mentioned in the chapter "Bhojana Kalpa" of the Kalpasthana, 22nd and 25th chapters of the Khilasthana.

Iodine deficiency disorders among school children of Malda, West Bengal, India.

A community-based, cross-sectional study was conducted among 2,392 school children, aged 8-10 years, in Malda district of West Bengal, India, in January 2001 to assess their iodine status. The children were selected through a multistage 30 cluster-sampling technique to determine the status of iodine deficiency disorders (IDD) using recommended quantifiable indicators. The prevalence of goitre was assessed clinically using the standard palpation method by the teachers of Community Medicine, and a total goitre rate of 11.

Bioremediation of DDT in soil by genetically improved strains of soil fungus Fusarium solani.

Bioremediation of DDT in soil by genetically improved recombinants of the soil fungus Fusarium solani was studied. The parent strains were isolated from soil enriched with DDD or DDE (immediate anaerobic and aerobic degradation products of DDT), as further degradation of these products are slow processes compared to the parent compound. These naturally occurring strains isolated from soil, however, are poor degraders of DDT and differed in their capability to degrade its metabolites such as DDD, DDE, DDOH and DBP and other organochlorine pesticides viz.

Regulation of gene expression and cell growth in vivo by tetracycline using the hollow fiber assay.

The hollow fiber assay presents a potentially unique tool to study the effects of regulated gene expression in cell lines that do not form tumors in vivo. The hollow fibers allow small molecules to pass freely through while keeping the cells within the fibers and segregated from host cells. OSp16.

S and G2 phase roles for Cdk2 revealed by inducible expression of a dominant-negative mutant in human cells.

Cyclin-dependent kinase 2 (Cdk2) is essential for initiation of DNA synthesis in higher eukaryotes. Biochemical studies in Xenopus egg extracts and microinjection studies in human cells have suggested an additional function for Cdk2 in activation of Cdk1 and entry into mitosis. To further examine the role of Cdk2 in human cells, we generated stable clones with inducible expression of wild-type and dominant-negative forms of the enzyme (Cdk2-wt and Cdk2-dn, respectively).

Induction of p21(WAF1/CIP1) and inhibition of Cdk2 mediated by the tumor suppressor p16(INK4a).

The tumor suppressor p16(INK4a) inhibits cyclin-dependent kinases 4 and 6. This activates the retinoblastoma protein (pRB) and, through incompletely understood events, arrests the cell division cycle. To permit biochemical analysis of the arrest, we generated U2-OS osteogenic sarcoma cell clones in which p16 transcription could be induced.

Temperature-induced conformational changes in prosomatostatin-II: implications for processing.

Somatostatin (SRIF) is a 14-residue peptide hormone synthesized in the hypothalamus and pancreatic islets. SRIF-14 and an N-terminally extended form, SRIF-28, are generated by the proteolytic processing of an approx. 102-residue precursor prosomatostatin (proSRIF) at a single set of paired basic residues (Arg-Lys) and at a monobasic (Arg) site respectively.

An assessment of missed opportunities for immunization in children and pregnant women attending different health facilities of a state hospital.

The present study carried out in Calcutta National Medical College, Calcutta, has revealed problem of missed opportunities of immunisation in children and pregnant women and factors contributing to the problem. The percentage of missed opportunity in children and pregnant woman are 30% and 7% respectively. The major cause in case of children were that all the vaccines are not administered in all the days of week (71.

Epidemiology of group-A streptococcal infection amongst children of different ethnic groups in Darjeeling.

The carrier rate of Group A Streptococcus (GAS) was studied amongst 932 children from 1+ upto 12 years of age in three major racial groups in the foothills of the Darjeeling district of West Bengal. It was altogether 13 per cent and was found to be evenly distributed in the three ethnic groups as Gurkhas: 11 per cent, Rajbanshis: 15 per cent and Cosmopolitans: 13 per cent, but the incidence of Rheumatic fever and/or Rheumatic heart diseases were unnoticed among the Gurkha children who also had significant low ASO titres.

Regulation of the acquisition of meiotic competence in the mouse: changes in the subcellular localization of cdc2, cyclin B1, cdc25C and wee1, and in the concentration of these proteins and their transcripts.

During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated these changes and furthermore indicate that an increase occurs in the nuclear concentration of each protein.

Glimpses of the advancement of medical science as depicted in the Mahäbhärata.

The Mahabharata of Vedavyasa is an encyclopaedic work, which has got some importance from the standpoint of Indian medical science also. According to it Ayurveda was a compulsory subject which was taught to everybody. Perhaps, Mahabharata is the first epic which presents the term Ayurveda.

Ca2+ dependent activation of rat uterine glycerylphosphorylcholine diesterase: presence of a positive modulator protein in uterine secretion.

The rat uterine secretory enzyme glycerylphosphorylcholine (GPC) diesterase (EC 3.1.4.

Association of glycerylphosphorylcholine with human sperm and effect of capacitation on their metabolism.

The presence and localization of glycerylphosphorylcholine (GPC) on the surface of human sperm, as well as the metabolism of its breakdown product L-glycerol 3 phosphate (G3P), were investigated. GPC was found to be associated with sperm after penetrating cervical mucus and was present after repeated washing of the sperm. GPC was partially released by treatment with 0.

Measuring the interactions of the determinants of health.

Considering environmental sanitation and health care services as most important determinants of health, this study was undertaken to know their impacts in an urban slum area of Calcutta. The families were studied in-depth and detail in relation to their health status in the form of morbidities and utilisation of preventive and promotive services. The health service impact was mainly assessed through comparative study between these who availed the services and to these who did not.

Intramedullary sodium cyanide injection on respiratory and vasomotor responses in cats.

To examine the effect of hypoxia confined to the ventrolateral medulla we microinjected NaCN into the cat medulla (1.0 mm below the ventral surface) unilaterally and investigated cardio-respiratory changes. We studied anesthetized artificially ventilated animals and measured the electrical activity of phrenic and cervical sympathetic nerves and blood pressure.

Drug consumption pattern in low socio-economic group in an urban community.

117 families, having a total population of 558 were studied regarding drug consumption pattern. It was found that the average no. of episodes per person was 0.

Detection of canine homologs of human MYC, BCL2, IGH, and TCRB genes by Southern blot analysis.

Subsets of non-Hodgkin's lymphoma in humans have been shown to involve activation of protooncogenes such as MYC and BCL2 resulting from chromosome translocation involving the IGH and TCR genes. Malignant lymphomas in the canine present histologic and clinical subsets similar to those in humans. To study the genetic nature of these lymphomas, we undertook this study to determine, by Southern blotting analysis, the extent of homology between the human and canine genes MYC, BCL2, IGH, and TCRB using human gene probes.

Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states.

The functional interaction of the estrogen-induced uterine enzyme glycerylphosphorylcholine (GPC) diesterase with epididymal rat sperm before and after incubation under capacitating conditions was investigated indirectly, by measuring the glycerol phosphate (GP) released on enzymatic hydrolysis of GPC and using oxygen consumption as a measure of oxidative phosphorylation by the sperm. Freshly released, washed sperm metabolized GP but not GPC. Utilization of GP was found to be inhibited by 2 microM oligomycin but was enhanced dose-dependently in the presence of the uncoupling agent, 2,4-dinitrophenol, indicating that the process was essentially similar to that occurring in somatic mitochondria in that it involved electron transport via ubiquinone and the cytochrome system and was coupled to ATP synthesis.