Optimized method for isolating highly purified and functional porcine aortic endothelial and smooth muscle cells.

Numerous protocols exist for isolating aortic endothelial and smooth muscle cells from small animals. However, establishing a protocol for isolating pure cell populations from large animal vessels that are more elastic has been challenging. We developed a simple sequential enzymatic approach to isolate highly purified populations of porcine aortic endothelial and smooth muscle cells.

Infrared spectra prediction and potential energy surface studies of methylarsine and methylstibine.

The vibrational frequencies, normal mode assignments and the internal-rotation and inversion potential energy surfaces of methylarsine and methylstibine are reported. The potential energy distribution (PED) for each frequency is found using Kim's Correspondence Rules of unified group theory and the program MOLVIB. The predominant motion of each PED is used to name the corresponding vibrational frequency.