Magnetophoretic circuits for digital control of single particles and cells.

The ability to manipulate small fluid droplets, colloidal particles and single cells with the precision and parallelization of modern-day computer hardware has profound applications for biochemical detection, gene sequencing, chemical synthesis and highly parallel analysis of single cells. Drawing inspiration from general circuit theory and magnetic bubble technology, here we demonstrate a class of integrated circuits for executing sequential and parallel, timed operations on an ensemble of single particles and cells. The integrated circuits are constructed from lithographically defined, overlaid patterns of magnetic film and current lines.

An organic substrate based magnetoresistive sensor for rapid bacteria detection.

A point-of-care diagnostic system has been developed to detect pathogenic bacteria rapidly, of which system contains a magnetoresistive (MR) sensor in cooperation with a magnetic bead coated by specific antibody against bacteria. MR sensor with Teflon passivation layer has been fabricated on organic substrate, being flexible and low cost material, and passivated by Teflon layer for maintaining flexibility. The performance of the MR sensor is demonstrated using Magnetospirillum magneticum AMB-1 and its detection limit was found to be 1.

Phytodegradation of the triphenylmethane dye Malachite Green mediated by cell suspension cultures of Blumea malcolmii Hook.

Cell cultures of Blumea malcolmii Hook., developed in the laboratory, rapidly decolorized textile industry effluent along with a variety of dyes with diverse structural properties. Most rapid decolorization was observed in case of Malachite Green (93.

Rhamnolipid from Pseudomonas desmolyticum NCIM-2112 and its role in the degradation of Brown 3REL.

The biosurfactant produced by Pseudomonas desmolyticum NCIM 2112 (Pd 2112) was confirmed as rhamnolipid based on the formation of dark blue halos around the colonies in CTAB-methylene blue agar plates and the content of rhamnose sugar. The average yield of rhamnolipid was 0.398 g/l/day when grown on hexadecane as sole carbon source.

Decolorization of the textile dyes using purified banana pulp polyphenol oxidase.

Polyphenol oxidase (PPO) purified using DEAE-cellulose and Biogel P-100 column chromatography from banana pulp showed 12.72-fold activity and 2.49% yield.

Production of polyhydroxyhexadecanoic acid by using waste biomass of Sphingobacterium sp. ATM generated after degradation of textile dye Direct Red 5B.

The degradation of textile effluent using microorganisms has been studied extensively, but disposal of generated biomass after dye degradation is a serious problem. The isolated Sphingobacterium sp. ATM was found to decolorize dye Direct Red 5B (DR5B) and simultaneously it produced polyhydroxyhexadecanoic acid (PHD).