Publications by authors named "Misuzu Shinzato"
Complete Genome Sequence of Carotenoid-Producing Enterococcus gilvus CR1, Isolated from Raw Cow's Milk.
Microbiol Resour Announc
September 2018
Article Synopsis
- Enterococcus gilvus CR1, a bacteria isolated from raw cow's milk, is capable of producing carotenoids.
- The complete genome sequencing of this strain was conducted using the PacBio RS II technology, resulting in a circular chromosome.
- The genome assembly consists of 2,863,043 base pairs, has a G+C content of 41.86%, and includes three plasmids containing carotenoid biosynthesis genes.
EG9 is a strain isolated from well-ripened cheese and accelerates free amino acid production during cheese ripening. Its complete genome sequence was determined using the PacBio RS II platform, revealing a single circular chromosome of 2,927,257 bp, a G+C content of 46.59%, and three plasmids.
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- - LQ80 is a bacterial strain found in liquid feed intended for pigs.
- - The complete genome sequence was obtained using the PacBio RS II sequencing technology.
- - The genome consists of a single circular chromosome that is 3,230,192 base pairs long, with a G+C content of 44.66%, and includes seven plasmids.
The complete genome sequence of sp. strain IBARAKI in a -containing culture was determined using the PacBio RS II platform. The genome is a single circular chromosome of 3,693,233 nucleotides (nt), with a GC content of 44%.
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- Subsp. G50 is a specific strain that has the ability to stimulate the immune system and was isolated from Napier grass.
- Researchers sequenced the entire genome of this strain using the advanced PacBio RS II sequencing platform.
- The genome is made up of a single circular chromosome that is 2,346,663 base pairs long, has a G+C content of 35.03%, and does not contain any plasmids.
We have developed and characterized a bacterial consortium that reductively dechlorinates trichloroethene to ethene. Quantitative PCR analysis for the 16S rRNA and reductive dehalogenase genes showed that the consortium is highly enriched with Dehalococcoides spp. that have two vinyl chloride reductive dehalogenase genes, bvcA and vcrA, and a trichloroethene reductive dehalogenase gene, tceA.
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- PacBio RS II is a groundbreaking third-generation DNA sequencer that can read single molecules of DNA in real-time without needing to amplify them.
- Its unique technology allows direct observation of DNA synthesis and offers key advantages like long read lengths, high accuracy, low bias, and the ability to analyze epigenetics.
- PacBio RS II is suitable for various applications including whole genome sequencing, targeted sequencing, and studying complex populations, making it valuable for both research and clinical use.
A free-living ciliate, Trimyema compressum, found in anoxic freshwater environments harbors methanogenic archaea and a bacterial symbiont named TC1 in its cytoplasm. Here, we report the complete genome sequence of the TC1 symbiont, consisting of a 1.59-Mb chromosome and a 35.
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- The complete genome sequence of Lactobacillus curvatus was obtained using the PacBio RS II technology.
- The genome consists of a single circular chromosome that is 1,848,756 base pairs long and has a G+C content of 42.1%.
- It contains low G+C regions and features 43 identical sequence pairs that are longer than 1,000 base pairs, with no plasmids found in the genome.
The first complete genome sequence of the type strain Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 50071(T)) was determined in a single contig by PacBio RS II. The genome (6,317,050 bp, G+C content of 66.52%) contained 10 sets of >1,000-bp identical sequence pairs and 183 tandem repeats.
View Article and Find Full Text PDFHere, we report the complete genome sequences of low-passage virulent and high-passage avirulent variants of pathogenic Leptospira interrogans serovar Manilae strain UP-MMC-NIID, a major causative agent of leptospirosis. While there were no major differences between the genome sequences, the levels of base modifications were higher in the avirulent variant.
View Article and Find Full Text PDFThe first complete genome sequence of Clostridium sporogenes DSM 795(T), a nontoxigenic surrogate for Clostridium botulinum, was determined in a single contig using the PacBio single-molecule real-time technology. The genome (4,142,990 bp; G+C content, 27.98%) included 86 sets of >1,000-bp identical sequence pairs and 380 tandem repeats.
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- The complete genome sequences of Staphylococcus aureus subsp. aureus Rosenbach 1884 strain DSM 20231(T) were analyzed using the PacBio RS II technology.
- The genome consists of a chromosome that is 2,755,072 base pairs long with a G+C content of 32.86%.
- It also includes a plasmid that measures 27,490 base pairs in length, with a G+C content of 30.69%.