[Interaction of various acceptors with oxygen anion-radicals in liver microsomes].

Interaction of acceptors with oxygen (O2) anion-radicals appearing in the microsomal chain of electron transfers, was studied. It was found that nitro blue tetrazolium (NBT) was reduced in liver microsomes both in the presence of O2 and through direct acceptance of electrons. The values of Km and Vmax for NBT and epinephrine interactions with microsomes were determined.

Interactions of some acceptors with superoxide anion radicals formed by the NADPH-specific flavoprotein in rat liver microsomal fractions.

In rat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (O2-), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific flavoprotein only directly. Nitro Blue Tetrazolium was reduced both by O2- and by the direct acceptance of electrons. Elevation of pH and addition of menadione shift the Nitro Blue Tetrazolium reduction towards the O2--dependent pathway.

[Peroxidative oxidation of lipids and the antioxidative activity of acetylene amine].

The study acetylene amines were capable of inhibiting the processes of lipids peroxidation both in the enzymatic and non-enzymatic peroxidation system. The inhibitory effect of the study compounds depended upon the degree of the compound unsaturation. An elevation of the unsaturation degree to a definite extent is attended also by an increased inhibitory action on the processes of the lipids peroxidation.

[Interrelation of oxygen anion-radical generation and lipid peroxidation in liver microsomes].

Consumption of O2-radicals and malondialdehyde accumulation was studied in the liver microsomes (LM) of rat after phenobarbital and 3-methylcholantrene induction, and in the LM of rabbits; there was revealed a suppression of lipid peroxidation, but a sufficiently high level of O2-radical generation. It is supposed that the absence of any direct association between the lipid peroxidation activity and the O2-radical generation in the LM of rats induced with the mentioned agents and in the LM of rabbits depended on increase in the antioxidant level.