[The multi-central clinical laboratory study of precision of the portative glucometer "Satellite Plus"].

The analysis of 482 blood samples of patients with diabetes mellitus type I and II and healthy persons was made using the portative glucometer "Satellite Plus". The comparison with reference analyzer was made. The results of clinical laboratory tests demonstrated that this glucometer meets the precision requirements of inter-national standard ISO 15197:2003 (97.

[The clinical laboratory evaluation of accuracy of portable glucometers "Satellite Express" and "Satellite Express mini"].

The portable glucometers "Satellite Express" and "Satellite Express mini" were applied during 35 days to analyze 200 blood samples of patients with diabetes mellitus type I and II and of healthy people. The results were compared with the data of reference analyzer The results of clinical laboratory trials revealed that these glucometers have enough degree of accuracy detection of glucose in capillary blood - 100% of results are within the permissible limits as directed by ISO 15197:2003. Furthermore, 98.

[Age-related features of ceruloplasmin biosynthesis and distribution in rats].

Biosynthesis of ceruloplasmin, a copper-containing glycoprotein, which plays an important role in copper transfer and bidirectional iron transport in vertebrates, was studied in 7-day old rats characterized by the embryonic type of copper metabolism. In addition to the liver, copper is synthesized in the lungs, brain, and kidneys. The appearance of labeled ceruloplasmin in the blood flow coincides with the time of liberation of de novo synthesized ceruloplasmin from the liver.

[Glucophot BP-"M" analyzer for blood analysis].

Miniature analyzer Glucophot BP-"M", intended for express measurements of glucose concentrations in capillary blood, was tested under clinical conditions. The reference method was the hexokinase method for glucose measurements on a Spectrum 11 biochemical analyzer (Abbott); in addition, glucose measurements were carried out on a One Touch-11 glucometer (Johnson-Johnson). The results evidence that the reproducibility and correctness of glucose analysis by the Glucophot BP-"M" analyzer both in health and disease met the requirements to laboratory measurements performed by "dry" chemical methods and demonstrated its high analytical reliability.

[Identification of a fragment of ceruloplasmin, interacting with copper-transporting Menkes ATPase].

The interaction was studied of ceruloplasmin (Cp, EC 1.16.3.

[Effects of infant formula feeding on the distribution of copper in the body of 8-day-old rats].

The oxidase activity of ceruloplasmin (Cp, EC 1.16.3.

[Kidney lesions in non-insulin dependent diabetes mellitus].

Because little is known about disordered metabolism of uric acid as a pathogenetic mechanism of renal damage in non-insulin-dependent diabetes mellitus (NIDDM) it was studied in 75 NIDDM and 48 IDDM patients. Clinical examination of the patients included evaluation of purin metabolism according to serum uric acid, diurnal urine excretion, uric acid clearance. Hyperuricemia occurred more frequently in NIDDM.

[Activities of antioxidant defense enzymes in children with insulin-dependent diabetes mellitus].

Activities of superoxide dismutase and glutathione peroxidase were measured in 60 children aged 5 to 15 with insulin-dependent diabetes of various degrees of compensation and duration. Measurements of glutathione peroxidase were found to be the most informative marker of the status of antioxidant defense system.

[Osteopenic syndrome in liquidators of the aftereffects of the accident at the Chernobyl power plant].

Bone system was examined in 112 liquidators; in 71 of these osseous tissue density was studied by osseous densitometer using bienergetic x-ray absorptiometry and in 45 x-ray examinations, analyses of biochemical and hormonal parameters were carried out. Spinal painful syndrome was detected in 61 to 82% of the examinees. For analysis of densitometric and x-ray parameters the liquidators were divided into 2 groups: group 1 included those who worked at the power plant in 1986 (55%) and group 2 were those who worked there in 1987-1989.

[Endemic goiter in Moscow and Moscow region].

Goiter prevalence and iodine excretion levels were assessed in schoolchildren aged 9 and 13 in the city of Moscow (n = 91) and the Moscow region, 60 km East of Moscow (n = 92). Goiter prevalence was evaluated by ultrasonography. In Moscow it was found to be 31.

[Bone tissue in patients with hyperprolactinemic hypogonadism].

Sixteen women with hyperprolactinemia were examined for Ca metabolism, osseous metabolism, mineral saturation of bone tissue in the thoracic portion of the spine and radius. Lowered mineral saturation of the thoracic vertebrae was detected in 69% of the examinees, that of the distal segment of the radius in 44%. Reduction of the mineral saturation of the spine was in negative correlation with the blood prolactin level and length of amenorrhea.

[Heart valve bioprostheses of the "Bionix" series. 6-year experience with the mitral valve replacement].

The main cause of cardiac valve bioprosthesis dysfunction is mechanical rupture of the cusps consequent upon the development of "fatigue" tensions and calcification of the biologic tissue. The technology of "Bionix" prostheses manufacturing implies prevention of these complications by means of supporting frameworks of variable rigidity (dumping stroke loads) and the use of a calcinosis inhibitor in the process of chemical stabilization. Experimental research confirms the certain effectiveness of such an approach to solving the problem of bioprosthesis durability.

[Changes in the liver circulation and kidney function during pulsatile and non-pulsatile perfusion].

Pulsatile flow perfusion (PFP) requires smaller volume of additional infusion. Extravascular hyperhydration is more marked after nonpulsatile flow perfusion (NPFP). In NPFP there is an increase in free water clearance, lower urinary osmolality and diuresis rate.

[Clinico-laboratory parallels in children with aggravated heredity of cholelithiasis].

A comprehensive examination of children with aggravated heredity regarding cholelithiasis revealed in the majority of them the first stage of cholelithiasis, namely abnormalities in physicochemical properties of the bile. It is demonstrated that formation of the pathology under consideration is promoted by combination of such factors as hereditary aggravation regarding cholelithiasis, complications of the perinatal period, vegetative disturbance, biliary dyskinesia, and derangement of the intestinal flora.

[An optical sensor for analyzing the flow of biological particles in chromatographic processes].

The design of an optic flow sensor operating in the UV spectrum is described. The sensor is intended to monitor changes in the size and/or concentration of light-diffusing particles against the background of low-molecular admixtures, as well as for analysis of these admixtures. The sensor has been employed in gel chromatography and adsorption chromatography of virus-containing suspensions.

[Fluorimetric determination of UDP-glucuronyltransferase and sulfotransferase activity in human blood cells].

Activity of UDP-glucuronosyl transferase and sulfotransferase, the enzymes participating in the second step of xenobiotics metabolism, was studied using 4-methyl umbellipherone in human granulocytes, thrombocytes and lymphocytes. A simple procedure is described for production of 3-phosphoadenosine-5-phosphosulfate coenzyme of sulfotransferase. The data obtained in the course of fluorimetric estimation of activity of these conjugases corresponded to the results of an isotope technique.

[A method of separate determination of enzymuria associated with impairment of glomerular filtration in kidney tissue].

Possibility of separate estimation of urinary enzymes after impairment of glomerular system and kidney tissue was considered theoretically. Activities of lactate dehydrogenase, alkaline phosphatase, aminopeptidase and gamma-glutamyl transferase were studied in blood serum and urine of II patients with nephrotic syndrome (4 patients with nephrotic form of glomerulonephritis and 7 patients with mixed form of glomerulonephritis). Urinary enzymes derived from blood serum constituted 20-30% of total enzymatic activity in urine of the patients with mixed form of glomerulonephritis and it was related only to the blood serum enzymes with relatively low molecular mass and high activity (alkaline phosphatase and LDH).

[Clinical significance of enzymuria in glomerulonephritis in children].

The authors presented the results of a study of enzymuria (cholinesterase, gamma-glutamine transferase, alkaline phosphatase, beta-galactosidase and lactate dehydrogenase with separate determination of N- and M-subunits) in 20 patients with a mixed form of glomerulonephritis (GN), 36 with the nephrotic form of GN and 13 patients with the hematuric form of GN. The clinical importance of the determination of enzymatic activity in the urine in GN of children lies in the recognition of the degree of damage of the glomerular filter as well as the nephrothelium. Basing on enzymuria pathophysiological syndromes found in various combinations in the above forms of GN were identified.