Rejection of the concept of hemiurid genus Pulmovermis (Digenea: Hemiuridae) and other taxonomic propositions: new morphological and molecular data regarding Lecithochirium cyanovitellosum (Coil and Kuntz, 1960) Urabe and Sokolov, comb. nov. (formerly Pulmovermis cyanovitellosus).

Pulmovermis cyanovitellosus Coil and Kuntz, 1960 is a species of hemiurid trematode that localizes in the lung of sea snakes, an unusual trait for this group of parasites. Recent molecular phylogenetic studies based on 28S rRNA gene sequences have shown that this species is closely related to members of the genus Lecithochirium Lühe, 1901. This finding is unexpected given that Pulmovermis Coil and Kuntz, 1960 and Lecithochirium are currently classified in different subfamilies of Hemiuridae (Pulmoverminae Sandars, 1961 vs.

Parasite fauna of Lake Biwa water system: A review on parasites using aquatic host organisms in their life cycle.

Lake Biwa, with its long geological history, has given rise to many endemic species, but only four endemic parasites are known from Lake Biwa and connected water areas. They are considered to have co-evolved with their endemic host species or to have become adapted to the pelagic ecosystem unique to Lake Biwa. The number of parasite species introduced into this water system is rising, facilitated not only by the introduction of new species but also through genetic analyses that have revealed new information about previously known species.

Proposal to synonymize Neoplagioporus Shimazu, 1990 with Urorchis Ozaki, 1927; taxonomic revision and the description of Urorchis takahaya n. sp.

Urorchis Ozaki, 1927 and Neoplagioporus Shimazu, 1990 (Digenea: Opecoelidae: Sphaerostomatinae) comprise species parasitic in freshwater fishes of eastern Asia, although the status of these genera is questionable. We revised these genera, primarily using evidence from a molecular phylogeny based on nuclear ribosomal DNA, including new sequences of four known and one new species. Urorchis was part of the clade of Neoplagioporus species, rendering the genus Neoplagioporus paraphyletic.

Genomic evidence of reproductive isolation among the Semisulcospira snails radiated in the ancient Lake Biwa.

Determining species boundaries within rapidly evolving species flocks is essential to understanding their evolutionary history but is often difficult to achieve due to the lack of clear diagnostic features. Ancient Lake Biwa harbours endemic snails in the genus Semisulcospira, a species flock with 19 described species. However, their morphological and genetic similarity cast doubt on the validity of their species status and their histories of explosive speciation.

TREMATODE SPECIES DETECTION AND QUANTIFICATION BY ENVIRONMENTAL DNA-qPCR ASSAY IN LAKE CHANY, RUSSIA.

Environmental DNA (eDNA) surveys promise to be a sensitive and powerful tool for the detection of trematodes. This can contribute to the limited studies on trematode ecology, specifically in aquatic ecosystems. Here, we developed species-specific primer and probe sets for Moliniella anceps, Opisthioglyphe ranae, and Plagiorchis multiglandularis cercariae and applied a novel eDNA qPCR assay to detect larval trematodes quantitatively.

A new species of the genus Paradistomum (Platyhelminthes: Trematoda) from Iwasaki's snail-eating snake Pareas iwasakii, with a note on morphological variations of Paradistomum megareceptaculum (Tamura, 1941).

Dicrocoeliid trematodes were detected from Iwasaki's snail-eating snake Pareas iwasakii in Iriomote Island, Okinawa Prefecture, Japan, and described as a new species Paradistomum dextra n. sp. in the present study.

Addressing the taxonomic confusion of Mesocoelium Odhner, 1910 (Trematoda: Plagiorchioidea: Mesocoeliidae) in Japanese urodelan and anuran amphibians.

Trematodes of the genus Mesocoelium Odhner, 1910 (Digenea: Plagiorchioidea: Mesocoeliidae) are globally distributed and parasitize amphibians, reptiles, or occasionally fishes. This genus is one of the most confusing taxa in trematodes because of its poor morphological features. In this study, we examined species of Mesocoelium collected from Japanese amphibians and found that they can be morphologically assigned to two species of Mesocoelium.

Molecular phylogenetic position and the life cycle of Phyllodistomum cyprini Feng et Wang, 1995 in Japan, with a note of a larval Phyllodistomum sp. from unionid mussels in North America.

A gorgoderid species, Phyllodistomum cyprini Feng et Wang, 1995 obtained from ureters of common carp, Cyprinus carpio in Japan is described both morphologically and molecularly. Its larval stage is a macrocercous cercaria found in a unionid mussel, Nodularia nipponensis first described by Urabe et al. (2015).

Characterization of the complete mitochondrial genome of Plagiorchis multiglandularis (Digenea, Plagiorchiidae): Comparison with the members of Xiphidiatan species and phylogenetic implications.

Plagiorchis multiglandularis Semenov, 1927 is a common fluke of birds and mammals, with significant impacts on animals and also human health. However, the systematics of Plagiorchiidae remain ambiguous. In the present study, the complete mitochondrial (mt) genome of P.

Integrative approach for discovering of the new species within the genus Allocreadium Looss, 1900 (Trematoda: Allocreadiidae) and framing of biogeographical hypotheses for the genus.

In July 2012 new Allocreadium species was isolated from Carassius gibelio caught in the Arsenyevka River, Primorsky region, Russia. Analyses on the morphometrics and internal organs' topology revealed that these worms are morphologically closest with A. isoporum but both species are independent according to high genetic distances based on the 28S gene fragment (5.

Two new kathlaniid species (Nematoda: Cosmocercoidea) parasitic in salamanders of the genus Andrias (Amphibia: Caudata: Cryptobranchidae).

The new nematode species, Falcaustra hanzaki n. sp. and Urodelnema takanoensis n.

The complete mitogenome of Echinoparyphium aconiatum (Digenea: Echinostomatidae) and a comparison with other digenean species.

Echinoparyphium aconiatum (Digenea: Echinostomatidae) is an intestinal parasite of anatid and snail-eating birds. In Eurasia, it is also common in lymnaeid snails, which may serve as the first and second intermediate hosts. The systematics of its genus, Echinoparyphium, have long been inadequate, with poor descriptions and extensive synonymy.

Three species of Kobayashi, 1921 (Digenea: Cryptogonimidae) in the East-Asian region: morphological and molecular data.

New data have been obtained for three representatives of Exorchis; Exorchis convictus sp. n., Exorchis oviformis and Exorchis sp.

n. gen., n. sp. (Digenea: Liolopidae) infecting the intestine of serrated hinged terrapin (), east African black mud turtle (), and South African helmeted turtle () and a phylogenetic hypothesis for liolopid genera.

We herein describe n. gen., n.

A record of juvenile Paragonimus skrjabini miyazakii from the urinary bladder of the Japanese toad, Bufo japonicus formosus.

We found juveniles of Paragonimus in the urinary bladder of a Japanese toad (Bufo japonicus formosus) captured in Kyoto. These were molecularly identified as Paragonimus skrjabini miyazakii Kamo, Nishida, Hatsushika et Tomimura 1961. This is the first report of P.

Systematics of Crepidostomum species from the Russian Far East and northern Japan, with description of a new species and validation of the genus Stephanophiala.

Current article touched upon the issue of the complicated taxonomic status of some species from the genus Crepidostomum collected from the freshwater fish in the rivers of Primorsky region, Sakhalin, and Hokkaido Islands. Primary morphological analyses showed affiliation of the worms to the species C. farionis (Müller, 1784) Lühe, 1909; C.

A new species of hemiuroidean trematode from Hatcheria macraei (Siluriformes, Trichomycteridae) and Heleobia hatcheri (Gastropoda, Cochliopidae) in a Patagonian River.

A new hemiuroidean species, Genarchella pichileufuensis n. sp. (Derogenidae: Halipeginae), was found in the stomach of the siluriform freshwater fish, Hatcheria macraei (Girard, 1855), in the Pichileufu River, Patagonia, Argentina.

Hemiuroidean trematodes from freshwater Patagonian fishes: description of a new species, distribution and molecular phylogeny.

A new trematode species, Derogenes lacustris Tsuchida, Flores, Viozzi, Rauque et Urabe n. sp. (Derogenidae: Derogeninae), from freshwater fishes is described using morphological and molecular approaches in Argentinean Patagonia.

Ancient drainage networks mediated a large-scale genetic introgression in the East Asian freshwater snails.

Biogeography and genetic variation of freshwater organisms are influenced not only by current freshwater connections but also by past drainage networks. The Seto Inland Sea is a shallow enclosed sea in Japan, but geological evidence showed that a large freshwater drainage had intermittently appeared in this area between the late Pliocene and Pleistocene. Here, we demonstrated that this paleodrainage greatly affected the genetic variation of the East Asian freshwater snails, spp.

Morphological and molecular studies of Eudiplozoon nipponicum (Goto, 1891) and Eudiplozoon kamegaii sp. n. (Monogenea; Diplozoidae).

Eudiplozoon nipponicum (Goto, 1891) Khotenovsky, 1985 (Monogenea: Diplozoidae), is known to parasitise Cyprinus carpio Linnaeus and species of Carassius. In this study, we conducted a taxonomic re-examination of E. nipponicum using genetic analysis and morphological comparisons from different host species from a single water system.

Description and molecular characteristics of Morishitium polonicum malayense Urabe, Nor Hashim & Uni, n. subsp. (Trematoda: Cyclocoelidae) from the Asian glossy starling, Aplonis panayensis strigata (Passeriformes: Sturnidae) in Peninsular Malaysia.

We describe Morishitium polonicum malayense n. subsp. from Asian glossy starlings (Aplonis panayensis strigata) (Horsfield, 1821) (Passeriformis: Sturnidae) caught in Malaysia.