Publications by authors named "Mirtha Biscoglio"
An effective, simple and low-cost pretreatment for culture clarification in tetanus toxoid production.
Prep Biochem Biotechnol
February 2019
Chemically inactivated tetanus toxin (tetanus toxoid, TT), purified from cultures of a virulent Clostridium tetani strain, is the active pharmaceutical ingredient of anti-tetanus vaccines. Culture clarification for TT production and is usually performed by filtration-based techniques. Final clarification of the culture supernatant is achieved by passage through 0.
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- Stimulation of amphibian oocytes by progesterone triggers a signaling pathway leading to the activation of Cdc2/cyclin B and reentry into meiosis.
- Cholesterol depletion, induced by methyl-beta-cyclodextrin (MbetaCD), impairs meiosis and alters membrane lipid order and localization of raft marker lipids, indicating the importance of membrane rafts in maturation.
- Ceramide serves as a strong maturation inducer that influences raft marker distribution, while progesterone does not affect membrane integrity, suggesting a complex relationship between cholesterol levels and hormone signaling in oocyte maturation.
We report the proteomic characterization of the venoms of two closely related pit vipers of the genus Lachesis, L. muta (South American Bushmaster) and L. stenophrys (Central American Bushmaster), and compare the toxin repertoire of the former revealed through a proteomic versus a transcriptomic approach.
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