Publications by authors named "Mirna Maalouf"
The present work reports on the preparation, characterization, and evaluation of a set of novel triphenyl-modified silica-based stationary phases without and with embedded ion-exchange sites for mixed-mode liquid chromatography. The three synthesized triphenyl phases differed in additionally incorporated ion-exchange sites. In one embodiment, allyltriphenylsilane was bonded to thiol-modified silica by thiol-ene click reaction, leading to particles with no ion-exchange sites.
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- Researchers developed a new mixed-mode stationary phase for protein high-performance liquid chromatography (HPLC) by combining octyl and 2-pyridylethyl ligands on silica, aiming to reduce unfavorable interactions seen in standard butyl-bonded silica.
- This method involved creating a dense polymeric siloxane layer on silica, and the performance of this phase was compared to traditional butyl-bonded and mixed-mode octyl/3-aminopropyl silica phases.
- The study found that the new mixed-mode phase produced better protein separation under acidic conditions (pH 3) by reducing retention times and improving peak shapes, thanks to reduced silanophilic interactions.