Publications by authors named "Mirna Giselle Moreira"
Article Synopsis
- Advanced diagnostic technologies like droplet digital PCR (ddPCR) provide a precise way to identify and measure pathogens in mini bronchoalveolar lavage samples for diagnosing ventilator-associated pneumonia (VAP).
- VAP is a serious lung infection in patients on ventilators, making accurate and timely diagnosis essential for effective treatment and reducing patient risk.
- The study found that ddPCR was more sensitive and able to detect pathogens in a higher percentage of clinical samples compared to traditional methods, while also avoiding issues with calibration curves.
Background: New Delhi Metallo-b-lactamase (NDM-1) is an enzyme emerging around the world conferring resistance to a wide range of β-lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the bla gene using the LOOP-mediated isothermal amplification technique (LAMP).
Methods: In all, 14 Gram-negative bacterial strains isolated from patients presenting pneumonia associated with mechanical ventilation were used for the bla standardization by LAMP.