Cell-polymer interactions of fluorescent polystyrene latex particles coated with thermosensitive poly(N-isopropylacrylamide) and poly(N-vinylcaprolactam) or grafted with poly(ethylene oxide)-macromonomer.

Cell-polymer interactions of thermosensitive poly(N-isopropylacrylamide) (PNIPAM) or poly(N-vinylcaprolactam) (PVCL) coated particles with RAW264.7 macrophages and intestinal Caco-2 cells were evaluated. Nanosized particles were prepared by modifying the surface of fluorescent polystyrene (FPS) particles with the thermosensitive polymer gels or with poly(ethylene oxide) (PEO)-macromonomer grafts.

Polystyrene latex particles coated with crosslinked poly(N-isopropylacrylamide).

Thermoresponsive colloidal particles were prepared by seeded precipitation polymerization of -isopropylacrylamide (NIPAM) in the presence of a crosslinking monomer, ,-methylenebisacrylamide (MBA), using polystyrene latex particles (ca. 50 nm in diameter) as seeds in aqueous dispersion. Phase transitions of the prepared poly(-isopropylacrylamide), PNIPAM, shells on polystyrene cores were studied in comparison to colloidal PNIPAM microgel particles, in HO and/or in DO by dynamic light scattering, microcalorimetry and by H NMR spectroscopy including the measurements of spin-lattice (T1) and spin-spin (T2) relaxation times for the protons of PNIPAM.