Publications by authors named "Miriam Mendez-Tavio"
Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct.
Mol Ther Methods Clin Dev
December 2020
Article Synopsis
- The study presents a method to create stable suspension cell lines from 293T cells that produce lentiviral vectors using a single DNA construct, which simplifies the production process compared to the traditional method involving multiple plasmids.
- These new cell lines can achieve high yields comparable to those from transient transfection and are designed to be easily scaled up in bioreactors, promoting efficient large-scale production.
- By streamlining the production process and improving stability and yield, this approach aims to enhance patient access to therapies developed using lentiviral vectors.