Publications by authors named "Miriam Gubernator"
Objective: We investigated the association between the functional, epigenetic, and expressional profile of human adventitial progenitor cells (APCs) and therapeutic activity in a model of limb ischemia.
Approach And Results: Antigenic and functional features were analyzed throughout passaging in 15 saphenous vein (SV)-derived APC lines, of which 10 from SV leftovers of coronary artery bypass graft surgery and 5 from varicose SV removal. Moreover, 5 SV-APC lines were transplanted (8×10(5) cells, IM) in mice with limb ischemia.
Article Synopsis
- This study focuses on the potential of adventitia-derived progenitor cells (APCs) from vein remnants of CABG surgery for cardiovascular regenerative therapy, particularly how oxidative stress affects their viability and function.
- APCs show better survival than endothelial cells after exposure to hydrogen peroxide due to their up-regulated antioxidant enzymes, notably superoxide dismutases (SODs), which also aid in promoting angiogenesis.
- The research highlights the importance of SOD3 in protecting APCs from oxidative damage and enhancing their therapeutic effectiveness in a model of limb ischemia, suggesting that APCs could be valuable for future cardiovascular treatments.
Coronary artery disease (CAD) is exceedingly prevalent and requires care optimization. Regenerative medicine holds promise to improve the clinical outcome of CAD patients. Current approach consists in subsidizing the infarcted heart with boluses of autologous stem cells from the bone marrow.
View Article and Find Full Text PDFRationale: Pericytes are key regulators of vascular maturation, but their value for cardiac repair remains unknown.
Objective: We investigated the therapeutic activity and mechanistic targets of saphenous vein-derived pericyte progenitor cells (SVPs) in a mouse myocardial infarction (MI) model.
Methods And Results: SVPs have a low immunogenic profile and are resistant to hypoxia/starvation (H/S).