Characterization of a GDS(L)-like hydrolase from Pleurotus sapidus with an unusual SGNH motif.

The GDS(L)-like lipase from the Basidiomycota Pleurotus sapidus (PSA_Lip) was heterologously expressed using Trichoderma reesei with an activity of 350 U L. The isoelectric point of 5.0 was determined by isoelectric focusing.