Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis.

Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products.

Phomactinine, the First Nitrogen-Bearing Phomactin, Produced by sp. CBMAI 1333.

Metabolomics analyses and improvement of growth conditions were applied toward diversification of phomactin terpenoids by the fungus sp. CBMAI 1333. Visualization of molecular networking results on Gephi assisted the observation of phomactin diversification and guided the isolation of new phomactin variants by applying a modified version of chemometrics based on a fractional factorial design.

Metabolomics Reveals a 26-Membered Macrolactone Produced by Endophytic spp. from Alcatrazes Island, Brazil.

Comparative metabolomics analysis of nonphytotoxic endophytic spp. isolated from endemic to Alcatrazes island (Brazil) and phytopathogenic spp. isolated from the mainland of Brazil revealed significant differences in chemical composition.

Metabolomics Reveals Minor Tambjamines in a Marine Invertebrate Food Chain.

Metabolomics analysis detected tambjamine alkaloids in aqueous and EtOAc extracts of the marine invertebrates , , , and . Among several tambjamines, the new amino acid derivatives tambjamines M-O (-) were identified by Marfey's advanced analysis, UPLC-MS/MS analyses, and total synthesis. The tambjamine diversity increased from the bryozoan to its nudibranch predators and and attained a higher diversity in , the nudibranch that preys upon and .

The chemistry and biology of guanidine natural products.

Covering: 2015 and 2016The chemistry and biology of natural guanidines isolated from microbial culture media, from marine invertebrates, as well as from terrestrial plants and animals, are reviewed. Emphasis is directed to the biosynthesis, total synthesis, ecological roles as well as on the evolution of guanidines isolated from natural sources.

Synthesis, characterization and antifungal activity of quaternary derivatives of chitosan on Aspergillus flavus.

Two series of new chitosan derivatives were synthesized by reaction of deacetylated chitosan (CH) with propyl (CH-Propyl) and pentyl (CH-Pentyl) trimethylammonium bromides to obtain derivatives with increasing degrees of substitution (DS). The derivatives were characterized by (1)H NMR and potentiometric titration techniques and their antifungal activities on the mycelial growth of Aspergillus flavus were investigated in vitro. The antifungal activities increase with DS and the more substituted derivatives of both series, CH-Propyl and CH-Pentyl, exhibited antifungal activities respectively three and six times higher than those obtained with commercial and deacetylated chitosan.