N-methyladenosine modification is not a general trait of viral RNA genomes.

Despite the nuclear localization of the mA machinery, the genomes of multiple exclusively-cytoplasmic RNA viruses, such as chikungunya (CHIKV) and dengue (DENV), are reported to be extensively mA-modified. However, these findings are mostly based on mA-Seq, an antibody-dependent technique with a high rate of false positives. Here, we address the presence of mA in CHIKV and DENV RNAs.

Controlling arbovirus infection: high-throughput transcriptome and proteome insights.

Arboviruses pose a significant threat to public health globally, demanding innovative approaches for their control. For this, a better understanding of the complex web of interactions established in arbovirus-infected mosquitoes is fundamental. High-throughput analyses allow a genome-wide view of arbovirus-induced alterations at different gene expression levels.

Vpx enhances innate immune responses independently of SAMHD1 during HIV-1 infection.

Background: The genomes of HIV-2 and some SIV strains contain the accessory gene vpx, which carries out several functions during infection, including the downregulation of SAMHD1. Vpx is also commonly used in experiments to increase HIV-1 infection efficiency in myeloid cells, particularly in studies that investigate the activation of antiviral pathways. However, the potential effects of Vpx on cellular innate immune signaling is not completely understood.