Publications by authors named "Miranda Willis"
Aim: To discuss inter-organisational collaboration in the context of the successful COVID-19 vaccination programme in North Central London (NCL).
Design: An action research study in 2023-2024.
Methods: Six action research cycles used mixed qualitative methods.
Many G-protein-coupled receptors trigger the synthesis of cAMP in order to transduce signals from the membrane into the cell cytoplasm. As stimulation of each receptor type results in a specific physiological outcome, compartmentalization of proteins that make, break, and are activated by cAMP underpin receptor-specific responses. Until 2002, it was thought that static compartmentalization of phosphodiesterase 4 (PDE4), conferred by N-terminal targeting sequences, was one way to shape intricate cAMP gradients that formed after receptor activation.
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- Raf-1 is an important activator of the ERK pathway that is regulated by the cAMP signaling system, specifically through the PKA enzyme that inhibits Raf-1 by phosphorylation.
- PDE8A, a cAMP-degrading phosphodiesterase, binds tightly to Raf-1, preventing PKA from inhibiting it and enhancing Raf-1's ability to activate ERK signaling.
- Disrupting the interaction between PDE8A and Raf-1 reduces ERK activation, with similar effects observed in genetically modified mice and Drosophila, suggesting that PDE8A plays a key role in regulating Raf-1 signaling in certain cells.