Impact of glyphosate herbicide exposure on sperm motility, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis).

The herbicide glyphosate is widely used in agricultural practices around the world, can reach aquatic environments, and potentially impact non-target organisms. This study aimed to investigate the effects of glyphosate exposure (both as the active ingredient and its formulated product) on sperm quality, fertilization success, and development of pejerrey (Odontesthes bonariensis), a native freshwater fish species from Argentina. Results revealed a statistically significant increase in sperm motility at the highest concentration of the formulated product.

Repair bond strength of composite resin to CAD/CAM glass-ceramic: influence of cleaning methods, surface treatments, and aging.

Objective: To evaluate the influence of different cleaning methods, surface treatments, and aging on the repair bond strength to a CAD/CAM glass-ceramic.

Materials And Methods: Forty-eight lithium disilicate CAD/CAM ceramic blocks were fabricated, sintered, and embedded in acrylic resin. After contamination with human saliva, they were divided according to the factors "Cleaning method" (Control-water/air spray, Air-particle abrasion with AlO, Ivoclean cleaning paste), "Surface treatment" (5% Hydrofluoric acid-HF + Silane, Monobond Etch & Prime-MEP), and "Aging" (thermocycling, no thermocycling).

Long-term effect of non-invasive brain stimulation on hemispatial neglect, functional outcomes, and mortality after stroke: ELETRON trial extend.

Introduction: Our primary clinical trial indicated that anodal stimulation of the right posterior parietal region associated with specific and perceptual task training was superior to placebo in reducing stroke-induced hemispatial neglect (HN) immediately after the treatment protocol. However, our primary study did not investigate whether this benefit was maintained in the long term after stroke. Therefore, this study aimed to evaluate the long-term effects of the protocol applied in the ELETRON trial on outcomes associated with HN, functionality, and mortality.

Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis.

Zoonotic visceral leishmaniasis is caused by () and dogs are the main domestic reservoir. This study compared the performance of parasitological tests using semi-automatic needle puncture (SANP) for collecting popliteal lymph node samples with samples collected from the same lymph node by fine needle aspiration puncture (FNAP) and by necropsy for the diagnosis of canine visceral leishmaniasis (CVL). Popliteal lymph node samples were collected from 30 CVL-seropositive dogs from an endemic region in Brazil.

The Effectiveness of Salivary Sampling for the Detection and Quantification of in Periodontitis Patients.

The objective was to evaluate using unstimulated saliva in detecting and to compare the saliva and subgingival and mucosa membrane occurrence of this periodontal pathogen in patients diagnosed with advanced periodontitis. Patients with advanced forms of periodontitis ( = 220; mean age: 54.03 ± 03 years) at stage III/IV were sampled.

Sex-specific fear acquisition following early life stress is linked to amygdala and hippocampal purine and glutamate metabolism.

Early life stress (ELS) can negatively impact health, increasing the risk of stress-related disorders, such as post-traumatic stress disorder (PTSD). Importantly, PTSD disproportionately affects women, emphasizing the critical need to explore how sex differences influence the genetic and metabolic neurobiological pathways underlying trauma-related behaviors. This study uses the limited bedding and nesting (LBN) paradigm to model ELS and investigate its sex-specific effects on fear memory formation.

A new species of stauromedusa, Calvadosia festivala (Cnidaria: Staurozoa: Kishinouyeidae) from India.

Staurozoa is a cnidarian class represented by marine benthic animals, mainly observed in intertidal and subtidal cold waters. As one of the few exceptions, in this study we described a tropical species, Calvadosia festivala n. sp.

Multilayer Ultratranslucent Monolithic Zirconia Crowns Treated With Multimode Universal Adhesive System Containing Zirconia Primer: A Clinical Report.

Introduction: Ultratranslucent zirconia combines high mechanical strength and esthetics, making it ideal for monolithic restorations. This case report describes a total rehabilitation of the upper dental arch with single-unit crowns made of multilayer ultratranslucent zirconia (Prettau 4 Anterior, Zirkonzahn), as well as the use of a simplified adhesive cementation protocol for this type of ceramic.

Materials And Methods: A 64-year-old female patient sought rehabilitative treatment, after planning in a semi-adjustable articulator, interim prostheses, from tooth 2 to 15, were installed to restore occlusal vertical dimension and esthetics.

Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.

The diagnosis of canine visceral leishmaniasis (CVL) presents a challenge due to a variety of non-specific clinical signs. The available tests have low sensitivity. This study aimed to standardize and evaluate the loop-mediated isothermal amplification technique with K26 target (K26-LAMP) for diagnosis of CVL in conjunctival swab (CS) DNA samples extracted through a silica column commercial kit (SW-kit) and boiling (SW-DB) and to compare sensitivity with conventional PCR (kDNA-cPCR) and quantitative real-time PCR (18S-qPCR).

Levoglucosenone as a starting material for cascade continuous-flow synthesis of (R)-γ-carboxy-γ-butyrolactone.

The global imperative to shift towards renewable and sustainable resources has spurred significant interest in exploring and utilizing platform chemicals derived from renewable feedstocks. Among these, levoglucosenone (LGO) and Cyrene™ have emerged as promising candidates. LGO, derived from the pyrolysis of cellulose and hemicellulose, exhibits structural versatility, making it an attractive starting material for various valuable products.

Zoonotic feline sporotrichosis: a small case cluster in Perth, Western Australia, and a review of previous feline cases from Australia.

Sporotrichosis is caused by species of the fungus Sporothrix that affect human and several animal species. The transmission of sporotrichosis is classically from an environmental source following penetrating injury. The alternative zoonotic route has gained attention due to the rapid and significant spread of cat-transmitted sporotrichosis in South America and Malaysia, resulting in several thousand human and feline cases in recent decades, especially in Brazil.

[Effect of previous exposure to COVID-19, occurrence of spikes, and type of vaccine on the humoral immune response of institutionalized older adults].

This study evaluated the explanatory factors of humoral immune response in older adults admitted to long-term care institutions in Buenos Aires, Argentina, up to 180 days after vaccination. An open-label, prospective, multicenter cohort study was conducted with volunteers who received two doses of the Sputnik V, Sinopharm, or AZD1222 vaccines. Plasma samples were analyzed at 0 and 21 days after the first dose, 21 days after the second dose, and 120 and 180 days after the first dose.

Decannulation protocol in pediatric patients: case series study.

Objective: The aim of this study was to describe the phases of a decannulation protocol and the results from its application in hospitalized children.

Methods: This is a retrospective, observational study. Data were collected from medical records of decannulated patients followed up in a pediatric hospital in Belo Horizonte, Minas Gerais between 2011 and 2021.

Experiences of living with severe mental disorder: narratives about the personal recovery process.

The article approaches the subject of recovery, a sociopolitical movement, field of studies and practices that aims, from the experience of people living with mental disorders, to promote a more optimistic outlook of the prognosis. Aiming to understand the recovery process of people who experience severe mental disorders, qualitative research was developed, based on Gadamerian hermeneutics. We conducted in-depth interviews, in the form of life narratives, with five people who recognize themselves in the process of recovery, and are part of a mental health patient group.

Articulated ankle-foot orthoses associated with home-based task-specific training improve functional mobility in patients with stroke: a randomized clinical trial.

Introduction: We compared fixed and articulated ankle-foot orthoses (AFOs) in home-based mobility tasks to assess short-term mobility, dynamic balance, quality of life, anxiety/depression, disability level, stroke severity, autonomy, human functioning, and patient satisfaction.

Methods: This was a two-arm, parallel-group, randomized controlled trial with concealed allocation, assessor blinding, and a complete case analysis involving patients with chronic stroke. The participants were randomized into two groups: fixed ( = 24) and articulated ( = 23) AFOs.

Antidepressant and anxiolytic effects of activating 5HT2A receptors in the anterior cingulate cortex and the theoretical mechanisms underlying them - A scoping review of available literature.

Psychedelic drugs that activate the 5HT2A receptor have long been the target of extensive clinical research, particularly in models of psychiatric illness. The aim of this literature review was to investigate the therapeutic effects of 5HT2A receptor activation in the anterior cingulate cortex (ACC) and the respective mechanisms that underlie them. Based on the available research, I suggest that 5HT2A receptors in the ACC exert profound changes in excitatory neurotransmission and brain network connectivity in a way that reduces anxious preoccupation and obsessional thoughts, as well as promoting cognitive flexibility and long-lasting mood improvements in anhedonia.

Effects of hypoxia on the reproductive endocrine axis of the pejerrey (Odontesthes bonariensis).

Recently, hypoxic areas have been identified in water bodies of the Pampas region due to human activity. The objective of this work was to study the effect of low concentrations of dissolved oxygen (hypoxia) on the reproductive endocrine axis of a pampas fish (Odontesthes bonariensis). Groups of 8 males and 8 females were subjected to severe hypoxia (2-3 mg l) and normoxia (7-9 mg l) in 3000 l tanks by duplicate during the reproductive season (spring).

Long-Term and Continuous Plasmonic Oligonucleotide Monitoring Enabled by Regeneration Approach.

The demand for continuous monitoring of biochemical markers for diagnostic purposes is increasing as it overcomes the limitations of traditional intermittent measurements. This study introduces a method for long-term, continuous plasmonic biosensing of oligonucleotides with high temporal resolution. Our method is based on a regeneration-based reversibility approach that ensures rapid reversibility in less than 1 minute, allowing the sensor to fully reset after each measurement.

In silico designing of multi-epitope vaccine against canine parvovirus using reverse vaccinology.

Canine parvovirus (CPV-2) is a highly contagious virus affecting dogs worldwide, posing a significant threat. The VP2 protein stands out as the predominant and highly immunogenic structural component of CPV-2. Soon after its emergence, CPV-2 was replaced by variants known as CPV-2a, 2b and 2c, marked by changes in amino acid residue 426 of VP2.