expansion of cord blood-derived endothelial cells using a novel xeno-free culture media.

Aim: Endothelial cells (ECs), isolated from peripheral blood (PB), bone marrow (BM) and cord blood (CB), are limited in numbers and expansion has had limited success. We used a novel serum-free medium (EndoGo) to evaluate effects on expansion of CB-derived ECs.

Materials & Methods: Flow cytometry and matrigel were used to determine expansion of ECs and for determination of the EC progenitor cell.