Mycotic aneurysm due to Magnusiomyces capitatus complicating a second liver transplant in a colonized patient.

This report presents an extremely rare case of fungal mycotic aneurysm due to Magnusiomyces capitatus in a 51-year-old immunocompromised woman. The diagnosis was based on multiple CT-scans and various samples positive for the pathogen identified by sequencing of the Internal Transcribed Spacer region. Long-term treatment using caspofungin for previous candidemia would have promoted the dissemination of this intrinsically echinocandin-resistant fungus, from colonization sites in the lung and rectal areas.

Reverse-transcriptase real-time PCR in the diagnostic strategy for invasive infections caused by .

The aim was to develop an RT-qPCR targeting and compare its performance to that of qPCR for the diagnosis of invasive aspergillosis (IA). Samples from patients of the Lyon University hospitals for whom a suspicion of IA led to the realization of an qPCR molecular diagnostic test over a 2-year period were included. The patients were classified according to the European Organization for Research and Treatment of Cancer/Mycoses Study Group (EORTC-MSGERC) criteria for suspected IA; RT-qPCR and qPCR assays were performed on all included samples.

Fungal Integrated Histomolecular Diagnosis Using Targeted Next-Generation Sequencing on Formalin-Fixed Paraffin-Embedded Tissues.

Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification. The objective of the present study was to develop targeted next-generation sequencing (NGS) on formalin-fixed tissue samples (FTs) to achieve a fungal integrated histomolecular diagnosis. Nucleic acid extraction was optimized on a first group of 30 FTs with Aspergillus fumigatus or infection by macrodissecting the microscopically identified fungal-rich area and comparing Qiagen and Promega extraction methods through DNA amplification by A.

Near-Threshold and Resonance Effects in Rotationally Inelastic Scattering of DO with -H.

We present a combined experimental and theoretical study on the rotationally inelastic scattering of heavy water, DO, with -H. Crossed-molecular beam measurements are performed in the collision energy range between 10 and 100 cm, corresponding to the near-threshold regime in which scattering resonances are most pronounced. State-to-state excitation cross-sections are obtained by probing three low-lying rotational levels of DO using the REMPI technique.

Design and characterization of a cryogenic linear Paul ion trap for ion-neutral reaction studies.

Ultra-high vacuum conditions are ideal for the study of trapped ions. They offer an almost perturbation-free environment, where ions confined in traps can be studied for extended periods of time-facilitating precision measurements and allowing infrequent events to be observed. However, if one wishes to study processes involving molecular ions, it is important to consider the effect of blackbody radiation (BBR).

Low-temperature reaction dynamics of paramagnetic species in the gas phase.

Radicals are abundant in a range of important gas-phase environments. They are prevalent in the atmosphere, in interstellar space, and in combustion processes. As such, understanding how radicals react is essential for the development of accurate models of the complex chemistry occurring in these gas-phase environments.

Characterisation of Early Positive Resistance Gene and Plasmidome in Pathogenic Strains Associated with Variable Phylogroups under Colistin Selection.

An antibiotic susceptibility monitoring programme was conducted from 2004 to 2010, resulting in a collection of 143 cultured from bovine faecal samples (diarrhoea) and milk-aliquots (mastitis). The isolates were subjected to whole-genome sequencing and were distributed in phylogroups A, B1, B2, C, D, E, and G with no correlation for particular genotypes with pathotypes. In fact, the population structure showed that the strains belonging to the different phylogroups matched broadly to ST complexes; however, the isolates are randomly associated with the diseases, highlighting the necessity to investigate the virulence factors more accurately in order to identify the mechanisms by which they cause disease.

Azole Resistance in Clinical and Environmental s Isolates from the French West Indies (Martinique).

The emergence of azole resistant spp., especially , has been described in several countries around the world with varying prevalence depending on the country. To our knowledge, azole resistance in spp.

A stand-alone magnetic guide for producing tuneable radical beams.

Radicals are prevalent in gas-phase environments such as the atmosphere, combustion systems, and the interstellar medium. To understand the properties of the processes occurring in these environments, it is helpful to study radical reaction systems in isolation-thereby avoiding competing reactions from impurities. There are very few methods for generating a pure beam of gas-phase radicals, and those that do exist involve complex setups.

Pulmonary aspergillosis in critically ill patients with Coronavirus Disease 2019 (COVID-19).

Unlabelled: Occurrence of putative invasive pulmonary aspergillosis was screened in 153 consecutive adult intensive care unit (ICU) patients with respiratory samples addressed for mycological diagnosis during a 6-week period at the emergence of coronavirus disease 2019 (COVID-19) pandemic. Positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) was observed for 106 patients (69.3%).

Microbial degradation of hydrophobic emerging contaminants from marine sediment slurries (Capbreton Canyon) to pure bacterial strain.

Despite emerging contaminants (ECs) are more and more monitored in environmental matrices, there is still a lack of data in marine ecosystems, especially on their fate and degradation potentials. In this work, for the first time, the degradation potential of synthetic musks (galaxolide and tonalide), UV filters (padimate O and octocrylene) and a pharmaceutical compound (carbamazepine) was studied in marine sediment samples, under laboratory conditions using sediment slurry incubations under biotic and abiotic conditions. Minimum half life times under biotic conditions were found at 21 days, 129 days and 199 days for padimate O, galaxolide and carbamazepine, respectively.

Simultaneous determination of 42 pharmaceuticals in seafood samples by solvent extraction coupled to liquid chromatography-tandem mass spectrometry.

An efficient and sensitive analytical method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the simultaneous determination of 42 pharmaceuticals belonging to different therapeutic classes (i.e. antibiotics, analgesics, anti-inflammatories, cardiovascular agents, anxiolytics and human indicators) in seafood samples.

Investigation of the effect of the adsorbent DAV131A on the propensity of moxifloxacin to induce simulated Clostridioides (Clostridium) difficile infection (CDI) in an in vitro human gut model.

Background: Clostridioides difficile infection (CDI) remains a high burden worldwide. DAV131A, a novel adsorbent, reduces residual gut antimicrobial levels, reducing CDI risk in animal models.

Objectives: We used a validated human gut model to investigate the efficacy of DAV131A in preventing moxifloxacin-induced CDI.

Cerebral phaeohyphomycosis due to Cladophialophora bantiana in a French Guianese child.

We report a case of cerebral phaeohyphomycosis, a fungal brain infection due to a dark (dematiaceous) fungi in a 6-year-old French Guyanese boy. The child presented fever and drowsiness due to several paraventricular brain abscesses. Neurological surgeries were performed to reduce intracranial hypertension and to obtain abscess biopsies.

Priority and emerging micropollutants distribution from coastal to continental slope sediments: A case study of Capbreton Submarine Canyon (North Atlantic Ocean).

Incising continental margins, submarine canyons are key issue for understanding shelf/deep sea exchange of particulate pollutant, impact on marine ecosystem and global geochemical cycling. The occurrence and distribution of 100 priority and emerging micropollutants were investigated in sediments within the first 25 km of the Capbreton submarine area. The most predominant compounds were polycyclic aromatic hydrocarbons (PAHs), trace metals and metalloid (TMs) (e.

Multi-residue analysis of 44 pharmaceutical compounds in environmental water samples by solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry.

A solid-phase extraction combined with a liquid chromatography-tandem mass spectrometry analysis has been developed and validated for the simultaneous determination of 44 pharmaceuticals belonging to different therapeutic classes (i.e., antibiotics, anti-inflammatories, cardiovascular agents, hormones, neuroleptics, and anxiolytics) in water samples.

Reaching the WHO target of testing persons in jails in prisons will need diverse efforts and resources.

Background: The Caribbean is the second most affected region in the world by human immunodeficiency virus (HIV), and HIV prevalence is significantly higher among persons in jails and prisons than in the free population. The aim of our study was to assess the screening rates of HIV, hepatitis B and C, syphilis and human T cell leukaemia virus type 1 among newly-arrived persons in 2014, at Ducos facility in Martinique and the testing process performance.

Methods: This is an observational monocentric study conducted within the prison's health unit.

Angiostrongylus costaricensis infection in Martinique, Lesser Antilles, from 2000 to 2017.

Human abdominal angiostrongyliasis (HAA) is a parasitic disease caused by the accidental ingestion of the nematode Angiostrongylus costaricensis in its larval form. Human infection can lead to severe ischemic and inflammatory intestinal lesions, sometimes complicated by life-threatening ileal perforations. Only one case had been reported in Martinique, an Island in the French Antilles, in 1988.

New Surge of Syphilis among Patients Living with Human Immunodeficiency Virus in Martinique in 2015.

A syphilis outbreak began in Martinique, French Antilles, in 2004, initially among men who had sex with men (MSM) and who were living with human immunodeficiency virus (HIV). The outbreak subsequently affected all groups at risk, leading to a first epidemic peak in 2008. After an initial decrease, the outbreak started growing again in 2014 among patients living with HIV with unprecedented incidence among MSM.

Protection of Hamsters from Mortality by Reducing Fecal Moxifloxacin Concentration with DAV131A in a Model of Moxifloxacin-Induced Clostridium difficile Colitis.

Lowering the gut exposure to antibiotics during treatments can prevent microbiota disruption. We evaluated the effects of an activated charcoal-based adsorbent, DAV131A, on the fecal free moxifloxacin concentration and mortality in a hamster model of moxifloxacin-induced infection. A total of 215 hamsters receiving moxifloxacin subcutaneously (day 1 [D] to D) were orally infected at D with spores.