Development of a double-antibody sandwich ELISA for rapid detection to C-peptide in human urine.

C-peptide level is recognized as an important indicator of diabetes diagnosis. A sensitive and specific double-antibody sandwich enzyme-linked immunosorbent assay for the detection of C-peptide based on double antibody sandwich method was studied in this paper. The rabbit and hen were innunized with PLL-C-peptide and BSA-C-peptide respectively to obtain specific Yolk antibody (IgY) and polyclonal antibody used to construct the sandwich ELISA for the measurement of C-peptide.

[Effect of DAPK1 gene transfection on high-metastasis non-small lung cancer cell PGCl3].

Background & Objective: Loss of activity of death- associated protein kinase 1 (DAPK1) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. However, the mechanism of DAPK1 inhibiting metastasis was unclear yet.

[A deletion mutant of plasminogen kringle 5 inhibits retinal capillary endothelial cell proliferation].

Objective: To obtain purified deletion mutant of plasminogen kringle 5 (K5) using gene mutation and genetic recombination methods and assess its anti-angiogenic activity in vitro.

Methods: A deletion mutant of K5 was obtained by deleting 15 amino acids from K5 while retaining all the 3 disulfide bonds. This K5 mutant (Mut1) was expressed in E.