In Situ Construction of Hollow Coral-Like Porous S-Doped g-CN/ZnInS S-Scheme Heterojunction for Efficient Photocatalytic Hydrogen Evolution.

The rational design of visible-light-responsive catalysts is crucial for converting solar energy into hydrogen energy to promote sustainable energy development. In this work, a C─S─C bond is introduced into g-CN (CN) through S doping. With the help of the flexible C─S─C bond under specific stimuli, a hollow coral-like porous structure of S-doped g-CN (S-CN) is synthesized for the first time.

Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy.

Translocation of 14-3-3 protein epsilon (14-3-3ε) was found to be involved in Triptolide (Tp)-induced inhibition of colorectal cancer (CRC) cell proliferation. However, the form of cell death induced by 14-3-3ε translocation and mechanisms underlying this effect remain unclear. This study employed label-free LC-MS/MS to identify 14-3-3ε-associated proteins in CRC cells treated with or without Tp.

Microwave-enhanced hydrogen production: a review.

Currently, the massive use of fossil fuels, which still serve as the dominant global energy, has led to the release of large amounts of greenhouse gases. Providing abundant, clean, and safe renewable energy is one of the major technical challenges for humankind. Nowadays, hydrogen-based energy is widely considered a potentially ideal energy carrier that could provide clean energy in the fields of transportation, heat and power generation, and energy storage systems, almost without any impact on the environment after consumption.

Network meta-analysis of curative efficacy of different acupuncture methods on obesity combined with insulin resistance.

Objective: This study aims to systematically evaluate the curative efficacy of different acupuncture methods in the treatment of obesity combined with insulin resistance in randomized clinical trials (RCTs) by network meta-analysis.

Methods: Four Chinese databases (CNKI, WanFang Data, VIP, and SinoMed) and four English databases (PubMed, Embase, the Cochrane Library, and www.clinicaltrial.

Flipping the Switch: Reverse-Demand Voltage-Sensitive Fluorophores.

Fluorescence microscopy with fluorescent reporters that respond to environmental cues is a powerful method for interrogating biochemistry and biophysics in living systems. Photoinduced electron transfer (PeT) is commonly used as a trigger to modulate fluorescence in response to changes in the biological environment. PeT-based indicators rely on PeT either into the excited state (acceptor PeT) or out of the excited state (donor PeT).

Status Quo and Research Trends of Neurosurgical Departments in China: Bibliometric and Scientometric Analyses.

Background: Modern neurosurgery is a relatively young discipline characterized by finesse and complexity. In recent years, neurosurgery in China has made continuous developments, with long-term progress and outstanding discoveries in many aspects of the field.

Objective: This scientometric investigation aimed to comprehensively provide insight into the development trends of neurosurgery in China, to demonstrate how the field has evolved.

Biomass-derived carbon from Ganoderma lucidum spore as a promising anode material for rapid potassium-ion storage.

The design and preparation of powerful anode materials are key to developing potassium-ion batteries. A biomass-based potassium anode material with a distinct hollow-cage structure was prepared by one-step carbonization. The target carbon exhibited a specific surface area of 104.

Si@SnS -Reduced Graphene Oxide Composite Anodes for High-Capacity Lithium-Ion Batteries.

Invited for this month's cover is the group of Weitang Yao at the Southwest University of Science and Technology. The image shows that developing low-cost and high-energy-density batteries is important for powering our city. The Si@SnS -rGO composites are good electrode materials for Li-ion batteries.

Si@SnS -Reduced Graphene Oxide Composite Anodes for High-Capacity Lithium-Ion Batteries.

One of the key challenges for the development of lithium-ion batteries is the preparation of high-performance anode materials. In this paper, a micro/nanostructured Si@SnS -rGO composite is reported in which Si nanoparticles with a particle size of 30 nm are electrostatically anchored on a 3D reduced graphene oxide (rGO) network and mixed with SnS . The step-wise lithiation/delithiation of SnS provided space-constraining effects to accommodate volume expansion and particle aggregation, thereby alleviating the volume expansion of Si during cycling as well as enhancing the structural stability, whereas the rGO in the 3D network stabilized the composite.

Marek's Disease Virus Activates the PI3K/Akt Pathway Through Interaction of Its Protein Meq With the P85 Subunit of PI3K to Promote Viral Replication.

It is known that viruses can active the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in host cells to support cell survival and viral replication; however, the role of PI3K/Akt signaling in the pathogenic mechanisms induced by Marek's disease virus (MDV) which causes a neoplastic Marek's disease in poultry, remains unknown. In this study, we showed that MDV activated the PI3K/Akt pathway in chicken embryo fibroblasts (CEFs) at the early phase of infection, whereas treatment with a PI3K inhibitor LY294002 prior to MDV infection decreased viral replication and DNA synthesis. Flow cytometry analysis showed that inhibition of the PI3K/Akt pathway could significantly increase apoptosis in MDV-infected host cells, indicating that activation of PI3K/Akt signaling could facilitate viral replication through support of cell survival during infection.

Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis.

TMZ resistance remains one of the main reasons why treatment of glioblastoma (GBM) fails. In order to investigate the underlying proteins and pathways associated with TMZ resistance, we conducted a cytoplasmic proteome research of U87 cells treated with TMZ for 1 week, followed by differentially expressed proteins (DEPs) screening, KEGG pathway analysis, protein-protein interaction (PPI) network construction, and validation of key candidate proteins in TCGA dataset. A total of 161 DEPs including 65 upregulated proteins and 96 downregulated proteins were identified.

Antitumor Activity and Mechanism of a Reverse Transcriptase Inhibitor, Dapivirine, in Glioblastoma.

Ethnopharmacological Relevance: Dapivirine is one of reverse transcriptase inhibitors (RTIs). It is the prototype of diarylpyrimidines (DAPY), formerly known as TMC120 or DAPY R147681 (IUPAC name: 4- [[4-(2, 4, 6-trimethylphenyl) amino]-2-pyrimidinyl] amino]-benzonitrile; CAS no.244767-67-7).

[MACF1 knockdown in glioblastoma multiforme cells increases temozolomide-induced cytotoxicity].

Objective: To investigate the role of microtubule-actin crosslinking factor 1 (MACF1) in the response of glioma cells to temozolomide (TMZ).

Methods: TMZ was applied to a human gliomablastoma cell line (U87) and changes in the protein expression and cellular localization were determined with Western blot, RT-PCR, and immunofluorescence. The responses of the cells with MACF1 expression knockdown by RNA interference to TMZ were assessed.

Expression of dynein, cytoplasmic 2, heavy chain 1 (DHC2) associated with glioblastoma cell resistance to temozolomide.

Temozolomide (TMZ) is the main chemotherapeutic drug utilized for the treatment of glioblastoma multiforme (GMB), however, drug resistance often leads to tumor recurrence and poor outcomes. GMB cell lines were treated with TMZ for up to two weeks and then subjected to proteomics analysis to identify the underlying molecular pathology that is associated with TMZ resistance. Proteomics data showed that TMZ altered expression of proteins that related to cytoskeleton structure and function, such as DHC2 and KIF2B.

AGE/RAGE/Akt pathway contributes to prostate cancer cell proliferation by promoting Rb phosphorylation and degradation.

Metabolomic research has revealed that metabolites play an important role in prostate cancer development and progression. Previous studies have suggested that prostate cancer cell proliferation is induced by advanced glycation end products (AGEs) exposure, but the mechanism of this induction remains unknown. This study investigated the molecular mechanisms underlying the proliferative response of prostate cancer cell to the interaction of AGEs and the receptor for advanced glycation end products (RAGE).

Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells.

Objective: To identify the protein regulation profile of recombinant human bone morphogenetic protein-2 (rhBMP-2)-induced osteogenic differentiation in beagle bone marrow stem cells (BMSCs).

Methods: Beagle BMSCs were isolated and cultured with or without rhBMP-2. Two-dimensional gel electrophoresis was used to determine the differences in protein expression in rhBMP-2-induced and non-induced BMSCs.

[Recombinant human bone morphogenetic protein-2 down-regulates LASP1 and up-regulates ferritin during osteogenic differentiation of beagle dog bone marrow mesenchymal stem cells].

Objective: To identify the proteins involved in osteogenic differentiation of beagle dog bone marrow mesenchymal stem cells (BMSCs) and explore its possible regulation mechanism of osteogenic differentiation of BMSCs.

Methods: Cultured beagle dog BMSCs were induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) for 7 days. The differentially expressed proteins between cells with osteogenic differentiation and control cells were identified by proteomics analysis based on two-dimensional gel electrophoresis.

Down-regulation of miR-200b-3p by low p73 contributes to the androgen-independence of prostate cancer cells.

Background: An increasing body of evidence indicates that microRNAs play critical roles in androgen-independent prostate cancer (AIPC) growth. However, the regulation of the expression of microRNAs in AIPC is not very clear. In this study, we investigated the role that the interaction between miR-200b-3p and p73 plays in the proliferation of AIPC.

Derepression of c-Fos caused by microRNA-139 down-regulation contributes to the metastasis of human hepatocellular carcinoma.

This study investigates whether the anti-metastasis effect of microRNA-139 (miR-139) on hepatocellular carcinoma (HCC) is mediated through regulating c-fos expression. The expression levels of miR-139 and c-fos in human HCC cell sublines with high (MHCC97H) and low (MHCC97L) spontaneous metastatic potentials were quantified using QPCR or Western blot. miR-139 mimics was transfected into MHCC97H cells to overexpress miR-139, and miR-139 inhibitor was transfected into MHCC97L cells to down-express miR-139.

Nix protein positively regulates NF-κB activation in gliomas.

Previous reports indicate that the NIX/BNIP3L gene acts as a pro-apoptotic factor by interacting with BCL2 and BCL-XL, playing an important role in hypoxia-dependent cell death and acting as a tumor suppressor. However, many studies also showed that NIX is linked to a protective role and cell survival in cancer cells. Nuclear factor-κB (NF-κB) can attenuate apoptosis in human cancers in response to chemotherapeutic agents and ionizing radiation.

Triptolide inhibits colon cancer cell proliferation and induces cleavage and translocation of 14-3-3 epsilon.

Triptolide is a diterpenoid triepoxide derived from the traditional Chinese medical herb Tripterygium wilfordii. In the present study, we demonstrated that this phytochemical attenuated colon cancer growth in vitro and in vivo. Using a proteomic approach, we found that 14-3-3 epsilon, a cell cycle- and apoptosis-related protein, was altered in colon cancer cells treated with triptolide.

Alteration of nuclear protein profiling for NIH-3T3 cells exposed to H₂O₂.

Oxidative stress is a threat to mammalian cells. To better understand the molecular response and mechanism underlying oxidative stress, we applied two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-fight mass spectrometry analysis to identify differential nuclear protein profiling of mouse fibroblast NIH-3T3 cells exposed to mild-H₂O₂. Thirteen differentially expressed proteins were identified by MS and two of them were further validated by Western blot.

Overexpression of T lymphoma invasion and metastasis 1 predict renal cell carcinoma metastasis and overall patient survival.

T lymphoma invasion and metastasis 1 (Tiam1) is a metastasis-related gene of T lymphoma that is also involved in the metastasis of a variety of other cancers, but little research has been done yet to test its usefulness as a prognostic marker in renal cell carcinoma (RCC). In the present study, we investigated Tiam1 expression and its prognostic significance in RCC. RCC surgical tissue samples were taken from 136 patients with RCC who had been followed up for 5 years.

[Estimating photosynthesized carbon distribution and inputs into belowground in a maize soil following 13C pulse-labeling].

Evaluating the contribution of maize growth to soil organic carbon is important for the understanding of the relationship of farmland carbon balance and agriculture production. 4 times of 13C pulse-labelling were used to estimate the photosynthesized carbon distribution at different development stages (seedling, elongation, heading and grain-filling) in maize-soil system, and quantify the carbon inputs into each part of belowground in whole growth season. The result indicated that the 13C retained aboveground reached its maximum: 80.