Metabolomic analysis of serum from rats following long-term intake of Chinese sausage.

Introduction: Owing to the contamination of chemical pollutants, especially nitrosamines and their precursors, in Chinese sausage, long-term intake of Chinese sausage may have potential health effects.

Objection: This study investigated the effects of long-term intake of Chinese sausage with different contaminations of -nitrosodimethylamine (NDMA) on rat liver and the potential biomarkers in the serum.

Methods: Serum metabolomic analysis was performed by gas chromatography-mass spectrometry at weeks 7, 17, 25, and 33; simultaneously, liver histopathological examination was conducted and its relationship with the serum metabolomics was also investigated.

Contamination of Chinese salted fish with volatile N-nitrosamines as determined by QuEChERS and gas chromatography-tandem mass spectrometry.

The QuEChERS sample preparation method and gas chromatography-tandem mass spectrometry were employed to determine nine volatile N-nitrosamines (VNAs) in Chinese salted fish. This method was validated by considering calibration plot linearity, selectivity, matrix effects, trueness, precision, limits of quantification and specificity. Fifty-four samples of Chinese salted fish obtained from five provinces were analyzed.