Background: The human adrenal gland is composed of the cortex and the medulla, which contain different function cells. The aim of this study was to build a 3D culture system for human adrenal glands.
Methods: Human fetal adrenal tissues were digested into a cell suspension culture and processed in three-phase 3D cultures.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
July 2024
Objective: To explore the serological characteristics and molecular mechanism underlying an individual with A3 phenotype.
Methods: A 27-year-old ethnic Han Chinese woman presented at the Fourth Affiliated Hospital of China Medical University on May 12, 2022 was selected as the study subject. ABO blood type was determined with standard serological techniques.
NaVO(PO)F (NVOPF) is gaining attention as a high-energy cathode candidate for sodium-ion batteries owing to its wide operating voltage, high energy density and excellent thermal stability. However, its intrinsic poor electrical conductivity results in its current sodium-storage performance being far below expectations. Herein, two-dimensional TiCT MXene nanosheets with excellent electrical conductivity are introduced to construct an interconnected conductive framework to tightly encapsulate NVOPF nanoparticles.
View Article and Find Full Text PDFFront Endocrinol (Lausanne)
December 2022
Human fetal adrenal glands produce substantial amounts of dehydroepiandrosterone (DHEA), which is one of the most important precursors of sex hormones. However, the underlying biological mechanism remains largely unknown. Herein, we sequenced human fetal adrenal glands and gonads from 7 to 14 gestational weeks (GW) 10× Genomics single-cell transcriptome techniques, reconstructed their location information by spatial transcriptomics.
View Article and Find Full Text PDFHLA-B*07:248 has one nucleotide change from HLA-B*07:18:01 where Tyrosine (Y) is changed to Serine (S).
View Article and Find Full Text PDFHerein, a label free and sensitive miRNA detection method with enhanced practical applicability was developed based on the locked nucleic acid (LNA) assisted repeated fishing amplification strategy. The working mechanism of the proposed method is as follows: 1) a DNA probe (i.e, L-DNA) with LNA bases is immobilized onto the surface of a gold foil.
View Article and Find Full Text PDFIn real-time quantitative polymerase chain reaction (PCR), the standard curve between threshold cycle and logarithm of template concentration is currently the gold standard for template quantification. The efficacy of this approach is limited by the necessary assumption that all samples are amplified with the same efficiency. To overcome this limitation, a new method has been proposed in this contribution for quantitative PCR with internal standard.
View Article and Find Full Text PDFPurposes: Data on PCP in patients with glomerular disease are rare. The aim of this study was to assess the predictors of PCP development, the risk factors for mortality and the incidence of acute kidney injury (AKI) when high-dose trimethoprim-sulphamethoxazole (TMP-SMX) was used in patients with non-transplant glomerular disease.
Methods: Forty-seven patients with PCP, as confirmed by positive results for Pneumocystis jirovecii DNA or Pneumocystis jirovecii cysts tested by a methenamine silver stain between January 1, 2003, and December 30, 2012, were retrospectively investigated.