Publications by authors named "Minwei Lin"
Article Synopsis
- The study introduces a method for producing the nonstandard amino acid para-nitro-L-phenylalanine (pN-Phe) within live bacteria, specifically Escherichia coli, allowing for easier access to this compound compared to chemical synthesis.
- The researchers engineered a metabolic pathway that used a unique nonheme diiron N-monooxygenase to generate pN-Phe, achieving a yield of 820 µM after optimization.
- They developed an orthogonal translation system that selectively incorporates the biosynthesized pN-Phe into a specific site of a reporter protein, establishing a new platform for creating nitrated proteins autonomously.
