Publications by authors named "Minuri Ratnayake"
Article Synopsis
- Non-ribosomal peptide synthesis generates various bioactive peptides through a modular system of catalytic domains that create diverse sequences.
- The chapter describes an in vitro biochemical assay designed to investigate the substrate specificity of condensation domains, which are crucial for elongating peptides.
- This assay simplifies the process by eliminating the need for adjacent adenylation domains and permits the testing of many synthetic substrates.
In a recent issue of Nature Chemical Biology, Folger et al. demonstrated a high-throughput approach for engineering peptide bond forming domains from non-ribosomal peptide synthesis. A non-ribosomal peptide synthetase module from surfactin biosynthesis was reprogrammed to accept a fatty acid substrate into peptide biosynthesis, thus illustrating the potential of this approach for generating novel bioactive peptides.
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