Adapting lipidomic sample processing methods for boars housed in commercial settings.

Multiple reaction monitoring (MRM) profiling is a sensitive method of lipid screening that has the capability to distinguish between different fertility phenotypes in gilts. However, MRM profiling has not yet been utilized to evaluate fertility phenotypes in boars. Markers indicative of fertility status in boars would be valuable as inclusion of subfertile boars in breeding programs results in a loss of efficiency and negative economic consequences.

The Role of Patient-Reported Outcomes to Measure Treatment Satisfaction in Drug Development.

Treatment satisfaction is a person's rating of his or her treatment experience, including processes and outcomes. It is directly related to treatment adherence, which may be predictive of treatment effectiveness in clinical and real-world research. Consequently, patient-reported outcome (PRO) instruments have been developed to incorporate patient experience throughout various stages of drug development and routine care.

Motives matter more with age: Adult age differences in response to sociomoral violations.

Moral judgments and emotional reactions to sociomoral violations are heavily impacted by a perpetrator's intentions and desires, which pose a threat to social harmony. Given that older adults are more motivated to maintain interpersonal harmony relative to younger adults, older adults may be more reactive to malicious desires. In three studies, we investigated adult age differences in moral judgments and emotional reactions to sociomoral violations.

Simplified Equilibrium Model for Exploring the Combined Influences of Concentration, Aggregate Shape, Excluded Volume, and Surface Adsorption upon Aggregation Propensity and Distribution of Globular Macromolecules.

A mesoscopic model for the equilibrium self-association of a globular macromolecule that may form oligomers of various shapes and unlimited sizes is presented. Allowance is made within this model for the effects of variation in the free energy of subunit contact within an oligomer of specified size and different shapes, the free energy of adsorption of an oligomer of specified size and shape to a planar surface, and the free energy of nonspecific excluded volume interaction between an oligomer of specified size and shape and an inert species occupying a specified fraction of total volume. The model is analytically soluble and permits rapid calculation and analysis of the effects of variation in each of the three free energy parameters upon the concentration dependence of the weight-average stoichiometry of the oligomer, the fraction of total macromolecule that is adsorbed, and the fraction of differently shaped oligomers that are adsorbed and in free solution.

Falling hard, but recovering resoundingly: Age differences in stressor reactivity and recovery.

Strength and vulnerability integration (SAVI) theory (Charles, 2010) posits that age differences in emotional experiences vary based on the distance from an emotionally eliciting event. Before and after a stressor, SAVI predicts that older age is related to motivational strivings that often result in higher levels of well-being. However, during stressor exposure, age differences are predicted to be attenuated or disappear completely.

National Technology Validation and Implementation Collaborative (NTVIC) policies and procedures for Forensic Investigative Genetic Genealogy (FIGG).

In 2022, the National Technology Validation and Implementation Collaborative (NTVIC) was established. Its mission is to collaborate across the US on validation, method development, and implementation. The NTVIC is comprised of 13 federal, state and local government crime laboratory leaders, joined by university researchers, and private technology and research companies.

B-cell receptor signaling induces proteasomal degradation of PDCD4 via MEK1/2 and mTORC1 in malignant B cells.

B-cell receptor (BCR) signaling plays a major role in the pathogenesis of B-cell malignancies and is an established target for therapy, including in chronic lymphocytic leukemia cells (CLL), the most common B-cell malignancy. We previously demonstrated that activation of BCR signaling in primary CLL cells downregulated expression of PDCD4, an inhibitor of the translational initiation factor eIF4A and a potential tumor suppressor in lymphoma. Regulation of the PDCD4/eIF4A axis appeared to be important for expression of the MYC oncoprotein as MYC mRNA translation was increased following BCR stimulation and MYC protein induction was repressed by pharmacological inhibition of eIF4A.

Influence of Nonspecific Interactions on Protein Associations: Implications for Biochemistry In Vivo.

The cellular interior is composed of a variety of microenvironments defined by distinct local compositions and composition-dependent intermolecular interactions. We review the various types of nonspecific interactions between proteins and between proteins and other macromolecules and supramolecular structures that influence the state of association and functional properties of a given protein existing within a particular microenvironment at a particular point in time. The present state of knowledge is summarized, and suggestions for fruitful directions of research are offered.

Effects of boar sperm antioxidant supplementation on fertility.

Use of cooled stored boar semen for artificial insemination accounts for 99% of inseminations in pork production sectors worldwide. The aim of the present study was to examine use of GameteGuard®-CP, a natural plant derived blend of antioxidants, supplemented in one commercially available semen extender on sperm quality, conception rate, farrowing rate, and litter size. Ejaculates from 16 commercial Duroc boars were used in a split-ejaculate single-sire artificial insemination study.

Translation and Cross-Cultural Adaptation of Quality of Life Scale in Patients with Onychomycosis.

(1) Background: Onychomycosis (OM) is a fungal nail infection, considered a risk factor for diabetic foot ulcers. It is associated with changes in quality of life, in terms of pain, self-confidence and self-esteem. The aim was to translate and adapt the OM quality of life questionnaire "OnyCOE-t-Quality Of Life Questionnaire Onychomycosis (Nail Fungal Condition)".

The Appraisal Approach to Aging and Emotion: An Integrative Theoretical Framework.

To advance our understanding of how emotional experience changes across the adult life span, we propose an integrative theoretical framework: the appraisal approach to aging and emotion (AAAE). AAAE posits that (a) age-related cognitive, motivational, and physical changes fundamentally change the appraisal system in certain ways, and that (b) older adults often deploy appraisal processes in different ways relative to their younger counterparts. As such, we hypothesize that these age-related changes to the appraisal process underlie the finding that older and younger adults tend to experience different emotions.

Reactive, Agentic, Apathetic, or Challenged? Aging, Emotion, and Coping During the COVID-19 Pandemic.

Background And Objectives: Advanced age is generally associated with improved emotional well-being, but the coronavirus 2019 pandemic unleashed a global stressor that gravely threatened the physical well-being and ostensibly challenged the emotional well-being of older adults disproportionately. The current study investigated differences in emotional experiences and coping strategies between younger and older adults during the pandemic, and whether these differences were accounted for by age differences in appraisal of the pandemic.

Research Design And Methods: We asked younger (n = 181) and older (n = 176) adult participants to report their stress, appraisals of the pandemic, emotions, and the ways in which they were coping with the pandemic.

Positivity helps the medicine go down: Leveraging framing and affective contexts to enhance the likelihood to take medications.

Affect can influence judgments and decision-making in multiple ways. One way is through (a) integral affect, or affect related to the choice at hand, and another way is through (b) incidental affect, or affect unrelated to the choice at hand. Research suggests integral affect influences risk-related decision-making, especially in the context of risky choice framing.

Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination.

The length of sperm tail midpiece, occupied by the mitochondrial sheath (MS), has been correlated with reproductive traits of mice, fish, and birds; however, it is not known whether such a correlation exists in higher order species such as domestic pigs. As the mitochondria provide for sperm motility and generate the fertility-affecting reactive oxygen species (ROS), we hypothesized that MS length correlates with boar semen parameters and artificial insemination (AI) fertility. Sperm samples collected from 57 boars and used for single sire AI were labeled with ProteoStat Aggresome probe (AGG; Enzo Life Sciences) for MS imaging by epifluorescence microscopy and image-based flow cytometry (IBFC).

Water Loss in Aging Erythrocytes Provides a Clue to a General Mechanism of Cellular Senescence.

Experimental evidence for age-dependent loss of intracellular water content as a widespread concomitant of cellular senescence is reviewed. Quantitative models are presented, indicating that an age-dependent increase in macromolecular crowding resulting from water loss may be responsible for three observed phenomena: a general age-dependent loss of intracellular protein solubility, a delayed and rapid appearance of high molecular weight aggregates, and an age-dependent transfer of intracellular protein from dilute to concentrated or condensed phases.

Implications of excluded volume for chemical inhibition of protein fibrillation.

Two quantitative models for the effect of high total macromolecular volume occupancy ('macromolecular crowding') upon the chemical inhibition of protein fibrillation are presented. The first assumes that fibrillation is reversible, and the second assumes that fibrillation is irreversible. Both models predict that small molecule inhibitors will be less effective in crowded media than in dilute media, whereas macromolecular inhibitors are likely to retain their efficiency in crowded media.

Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance.

There is a need to identify subfertile boars before they enter into the breeding herd. Seminal plasma proteins are essential for normal sperm function and transport and play an important role in fertilization. The objective of this study was to use liquid chromatography tandem mass spectrometry for shotgun proteome analysis to investigate whether differences in boar fertility phenotype can be differentiated by seminal plasma protein abundance.

Simple Calculation of Phase Diagrams for Liquid-Liquid Phase Separation in Solutions of Two Macromolecular Solute Species.

A simple method is described for the calculation of two- and three-dimensional phase diagrams describing stability and coexistence curves or surfaces separating one- and two-phase regions in composition/temperature space of a solution containing solute species 1 and 2. The calculation requires a quantitative description of the intermolecular potentials of mean force acting between like (1-1 and 2-2) and unlike (1-2) species. Example calculations are carried out for solutions of species interacting via spherically symmetric square-well potentials as first-order models for protein-protein interaction.

The Cumulative Effect of Surface Adsorption and Excluded Volume in 2D and 3D on Protein Fibrillation.

A refined mesoscopic model for the cumulative effect of repulsive excluded volume protein-protein interaction and attractive protein-surface interaction upon the properties of a trace protein capable of fiber formation is presented. The model predicts that very small changes in the magnitude of bulk volume occupancy or the strength of protein-surface attraction may result in very large changes in the extent of trace protein fibrillation and the distribution of trace protein between bulk and adsorbed phases.