Geraniol (GER) attenuated chronic sleep restriction (CSR)-induced neuroinflammation in adolescent mice.

Sleep insufficiency is a significant health problem worldwide, and adolescent sleep restriction (SR) could induce multiple neurodevelopmental disorders in the central nervous system (CNS). Microglial-mediated neuroinflammation plays a vital role in multiple neurological diseases, and recent research showed the regulation effect of immunoproteasome on microglia functions. Geraniol (GER), an important ingredient in many essential oils, possesses diverse pharmacological properties like anti-inflammatory and antioxidant.

Phosphorescence Lifetime Imaging of Labile Zn in Mitochondria via a Phosphorescent Iridium(III) Complex.

Phosphorescence lifetime Zn imaging possesses the advantage over normal fluorescence imaging in offering the more accurate temporal-spatial Zn information. Herein, we report a new phosphorescent cyclometalated Ir(III) complex with a Zn-chelator bearing 1,10-phenanthrolin acting as ancillary ligand, Zin-IrDPA, which displays the specific Zn-induced enhancement of phosphorescence and phosphorescence lifetime, and the mitochondria-targeting ability. Moreover, its Zn-induced phosphorescence lifetime enhancement factor is not affected by medium lipophilicity, viscosity, polarity, and especially ambient oxygen.

Conversion efficiency and oil quality of low-lipid high-protein and high-lipid low-protein microalgae via hydrothermal liquefaction.

Hydrothermal liquefaction (HTL) is a promising technology for converting algae into biocrude oil. Here, HTL of a low-lipid high-protein microalgae (Nannochloropsis sp.) and a high-lipid low-protein microalgae (Chlorella sp.

Enhancements of thermal conductivities with Cu, CuO, and carbon nanotube nanofluids and application of MWNT/water nanofluid on a water chiller system.

In this study, enhancements of thermal conductivities of ethylene glycol, water, and synthetic engine oil in the presence of copper (Cu), copper oxide (CuO), and multi-walled carbon nanotube (MWNT) are investigated using both physical mixing method (two-step method) and chemical reduction method (one-step method). The chemical reduction method is, however, used only for nanofluid containing Cu nanoparticle in water. The thermal conductivities of the nanofluids are measured by a modified transient hot wire method.

Correcting for the inner filter effect in measurements of fluorescent proteins in high-cell-density cultures.

Fluorescent proteins (FPs), such as green fluorescent protein (GFP) and its variants, are well-developed visible markers for analyzing bioprocesses. Accurate measurement of fluorescence emitted from FPs in whole cells is complicated by the inner filter effect (IFE), which is caused by intracellular light absorption and scattering by cell particles. The IFE causes nonlinearity between fluorescence intensity and fluorophore concentrations in FP-harboring cells and can significantly influence the accuracy of FP-based analysis, especially at high cell densities.

Temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase.

By constructing the expression system for fusion protein of GFPmut1 (a green fluorescent protein mutant) with the hyperthermophilic xylanase obtained from Dictyoglomus thermophilum Rt46B.1, the effects of temperature on the fluorescence of GFP and its relationship with the activities of GFP-fused xylanase have been studied. The fluorescence intensities of both GFP and GFP-xylanase have proved to be thermally sensitive, with the thermal sensitivity of the fluorescence intensity of GFP-xylanase being 15% higher than that of GFP.

HIV incidence, retention rate, and baseline predictors of HIV incidence and retention in a prospective cohort study of injection drug users in Xinjiang, China.

Objective: To determine HIV seroincidence, study participant retention rate, and baseline predictors of HIV incidence and study retention among high-risk injection drug users (IDUs) in Xinjiang, China.

Methods: A total of 508 eligible seronegative high-risk IDUs were enrolled. Study participants were tested for HIV-1 and counseled at the baseline, 6-month, and 12-month follow-up visits.

[A minimally invasive approach in the treatment of complicated anal fistula through spatium intermuscular of anal sphincter].

Objective: To investigate the optimal operative approach for the complicated anal fistula.

Methods: One hundred and ninety-two cases with complicated anal fistula were randomly divided into minimally invasive operation group (through spatium intermuscular of anal sphincter) and fistula resection group. The operation time, bleeding time during and after operation, pain lasting time, healing time of incision, area of anal scar, anal malformation and function and post operative recurrence were observed and compared between the two groups.

[Construction of plasmid with green fluorescent protein gene for analysis of yeasts in yeast wastewater treatment].

Green fluorescent protein (GFP) can be utilized in analysis of the characteristics and distribution of a targeted strain in microbial communities. This study is the first step to establish a dynamic yeast monitoring technique in a wastewater treatment system using yeast by constructing a fluorescent yeast containing gfp gene. The gfp gene was inserted into pACT1-URA3, a powerful plasmid for introducing a foreign gene into Candida boidinii, and then transformed into E.