[Construction of quantitative real-time PCR detection system of transgenic tomato line Zeneca B,Da,F].

Objective: To construct the plasmid reference molecules for detection of transgenic tomato line Zeneca B,Da,F using quantitative real-time PCR(qPCR).

Methods: Three plasmid reference molecules pEasy-T3-APX, pEasy-T3-16A and pEasy-T3-16S were cloned based on reverse genetics, which contain the target fragments of tomato endogenous reference gene apx (ERG-apx), gene-specific sequence of pg(GS-pg) and construct-specific sequence of vectors pJR16S/pJR16A (CS-16S/CS-16A) of Zeneca B,Da,F, respectively. Primers and Taqman probes were designed by Beacon Designer 7.

The Effects of Q-Switched Nd:YAG Laser Irradiation in the Wavelength of 1064nm and 532nm on Guinea Pigs' Skin Tissue.

Unlabelled: To investigate the effect of Q-switched Nd: YAG laser irradiation in the wavelength of 1064nm and 532nm on Guinea Pigs' skin tissue, according to the method and dose which are used clinically to treat pigmentary skin diseases, so as to provide some reference and foundation for the clinical usage of Q-switched Nd: YAG laser.

Methods: three different doses of Q-switched Nd:YAG laser in the wavelength of 1064nm and 532nm were used repectively for in vitro irradiation on Guinea Pigs, then the histological change of the skin tissue were observed and analyzed with microscope and image analysis instrument.

Results: Each experimental group showed obvious histological changes, and with the increase in doses, the extent of change became larger.