Possible mechanisms for the effect of protein sensitization on functional properties of the isolated m. soleus and m. EDL from mice.

We studied possible involvement of ATP in the influence of protein sensitization on contractile function and non-quantum secretion in the end-plate of isolated skeletal muscles from mouse leg. The dynamic vector of muscle contraction force was shown to correlate with changes in non-quantum secretion of acetylcholine under various conditions of experimental pathology. However, the degree of these changes was lower in sensitized animals.

Change in lymph flow during low-grade fever and febrile fever under experimental conditions.

Experiments on rats showed that low-grade fever is accompanied by a decrease in the central lymph flow rate, which results from inactivation of lymphangion components in lymphatic microvessels of the small intestine mesentery. Lymph flow rate was shown to increase during pyrogenal-induced fever, which did not depend on the duration of the disease.

Reactivity of tracheal smooth muscles in albino rats with experimental diabetes mellitus treated with a new complex compound of oxovanadium (IV) and isonicotinic acid hydrazide.

We studied functional properties of tracheal smooth muscle cells in rats with diabetes mellitus. Reactivity of tracheal smooth muscles increased in rats with experimental alloxan-induced diabetes mellitus. A new complex compound of oxovanadium (IV) and isonicotinic acid hydrazide affected reactivity of tracheal smooth muscles in albino rats with experimental type I diabetes mellitus.

Correction of lipid composition of the lymph and blood during immediate type hypersensitivity.

Combination therapy with hydrocortisone and norepinephrine improved resorption and transport functions of the lymphatic system during anaphylactic shock, which was accompanied by a considerable increase in the contents of total lipids, phospholipids, cholesterol, nonesterified fatty acids, and malonic dialdehyde (lipid peroxidation product) in the lymph and blood. It should be emphasized that lipid concentration in the lymph increased to a greater extent than in the blood.

Changes in the count of pancreatic beta- and alpha-cells and blood glucose level in rats with alloxan-induced diabetes.

We estimated the count of pancreatic alpha- and beta-cells and blood glucose level at various stages of alloxan-induced diabetes in rats. Alloxan decreased the count of insulin-producing beta-cells, but increased the number of glucagon-secreting alpha-cells in the pancreas (week 1 of diabetes). These changes were accompanied by hyperglycemia.

Key role of mitochondria in apoptosis of lymphocytes.

Changes in the mitochondrial potential, expression of phosphatidylserine, parameters of direct and lateral light scattering, and DNA fragmentation during spontaneous and induced apoptosis in peripheral blood lymphocytes were studied by flow cytofluorometry. Dexamethasone and Ca2+ ionophore A23187 served as inductors of apoptosis. A decrease in the mitochondrial potential is an early sign of spontaneous and induced apoptosis.

[Indications for hypoxitherapy].

Hypoxytherapy is the non-medicinal therapeutic method using gaseous hypoxic mixture (GHM) with decreased oxygen contents. The method is based on the activation of body protective mechanisms, phagocytosis stimulation, microcirculation improvement, sedative effect. GHM therapy is indicated in neurosis, CHD, hypertension, chronic pulmonary obstructive disease, to prevent from side effect of ionizing radiation, to increase the resistance during complex therapy of oncologic patients.

Correction of lymph circulation during immediate hypersensitivity reaction.

We measured lymph flow rate in the thoracic lymphatic duct of dogs with anaphylactic shock receiving mono- or combination therapy with norepinephrine and hydrocortisone. Intensification of lymph circulation improved resorption and transport of metabolic products from the interstitial space through lymphatic vessels and stimulated exchange processes in the blood and tissues during allergic alterations.

Allergic alteration in the organism is associated with disturbances in lipid composition of the lymph.

Anaphylactic shock causes changes in the transport chain of lipid metabolism in the blood-tissue-lymph-blood system. During the early periods of shock, the changes in lipid composition of the lymph and blood result from increased permeability of the blood-tissue barriers, while at later terms, these processes are primarily determined by metabolic changes.

[Transferase contents in lymph and blood in fever reaction].

The aim of the present investigation was the study of the content of alanine, asparagine-transaminases, gamma-glutamyl transferase and their isoenzymes, as well as leucine aminotransferase in the lymph of thoracic duct, hepatic and intestinal lymph and peripheral blood in dynamics of fever reaction of various duration in the experiments on rabbits. Irrespective of its duration, the fever was followed by a significant activation of the enzymes in the body fluids. However, in many-day fever reaction, a rise of enzymes level in the lymph was more prolonged than that in the blood.

[Effect of the fever reaction on lymph cytologic composition and lymph node structure].

The authors studied the cytologic composition of afferent and efferent lymph and the morphology of the popliteal lymph node during a fever reaction of various duration. The fever reaction was attended by quantitative and qualitative in the cytologic composition of both lymph types. Reduction of the leukocyte count in the first hours of fever was replaced by its increase: the percentage of small- and moderate-size lymphocytes reduced, while that of poorly-differentiated cells--blasts and prolymphocytes--increased.

[Morpho-functional changes in the lymph nodes during fever reaction].

Lymph nodes (mesenteric, popliteal, cervical) of rabbits in fever reaction of different duration have been studied in our work. As a whole morpho-functional changes in lymph nodes in fever reaction indicate the increase of their functional activity: hyperplasia of lymphatic substance with the growth of lymphocytes number and slightly differentiated lymphoid cells in follicles and paracortical zone, hyperplasia of pulposus bands, the signs of macrophagal reaction and plasmatization of lymph nodes are to be observed and all these create prerequisites for the increase of tensity of cellular and humoral immunity. Simultaneously the signs of destruction of cellular elements--lymphocytolysis in the porta tract and the growth of number of PAS-positive cells in the parenchyma of the nodes take place.

[Phosphatase activity in the lymph during a fever reaction].

Activity of acid and alkaline phosphatases, as well as isoenzymes of alkaline phosphatase in the lymph of the thoracic lymphatic duct, hepatic lymph and the peripheral blood have been studied on rabbits in the dynamics of the fever reaction of different duration. The fever reaction was followed by enzyme activity increase in all the body biologic fluids. However the degree of increase of their activity in the lymph was greater that that in the blood.

[Cytological composition of lymph in fever reaction].

This work deals with an investigation into the lymph cytologic composition of thoracic lymph duct of rabbits in fever reaction (FR) of various duration. FR was accompanied by quantitative and qualitative shifts in lymph cytologic composition. There was an alternative rise and fall of the leucocyte number in the first hours of fever.

[Activity of several carbohydrate exchange enzymes in the lymph during fever reaction].

This work includes a comparative study of aldolase enzyme activity, LDG and its isoenzymes in thoracic duct lymph and blood serum of rabbits in fever reaction dynamics of various duration. Irrespective of its duration the fever was followed by a significant activation of investigated enzymes in the body liquids. However, in many day fever reaction a rise of LDG level in the lymph was more prolonged then that in the blood.

[Effect of the fever reaction on the kallikrein-kinin system of the lymph and blood].

The dynamics of kallikrein-kinin system components in the lymph of thoracic duct and blood following fever reaction of various duration has been studied in the experiment on rabbits. The experiments have shown that in prolonged fever reaction there are qualitative disorders in kinin system components ratio indicative of break in the system of regulatory links, its exhaustion and transformation of the physiological reactions into the pathogenic ones. One may assume that changes in kallikrein-kinin system activity in body fluid are the links in the complex of pathogenic disorders in the organ and system functional activity during prolonged fever reaction.

[Amino acid composition of the lymph and blood in fever reactions of different durations].

The amino acid composition of the thoracic duct lymph and blood following fever reactions (FR) of various duration has been studied in the experiments on rabbits. The experiments have shown that some changes in protein metabolism in FR are primarily manifested in amino acid lymph composition. Irrespective of FR duration the amino acid content in the lymph almost always increases, which confirms the opinion concerning catabolic process predominance in protein metabolism during FR.