Potential habitat suitability of Liberibacter asiaticus and genetic diversity of its prophages across China.

Unlabelled: Huanglongbing (HLB) is a severe citrus disease in China caused by Liberibacter asiaticus (Las). Since its initial identification, the pathogen has spread to 10 mainland provinces in China and caused devastating loss. Three distinct prophage types have been identified in Las; however, their distribution and diversity in China remain inadequately understood.

Dodder-transmitted mobile systemic signals activate a salt-stress response characterized by a transcriptome change in .

Citrus is an essential horticultural fruit whose yield and quality are affected by salinity all over the world. The recognition and adaptive regulation of citrus against salt stress are important areas for cultivar improvement, but the vascular system signal transduction mechanism of the plant response to salt stress remains elusive. In this study, we constructed a dodder () linked Hamlin sweet orange () plant community in which deliver a vascular signal through the dodder in response to salt stress.

A Fluorescent Reporter-Based Evaluation Assay for Antibacterial Components Against subsp. .

subsp. () is the agent of citrus bacterial canker (CBC) disease, which has significantly reduced citrus quantity and quality in many producing areas worldwide. Copper-based bactericides are the primary products for CBC control and management, but the problems derived from copper-resistant and environmental contamination have become issues of anxiety.

A Novel Phage (CLasMV1) From " Liberibacter asiaticus".

" Liberibacter asiaticus" (CLas) is an unculturable phloem-limited α-proteobacterium associated with citrus Huanglongbing (HLB; yellow shoot disease). HLB is currently threatening citrus production worldwide. Understanding the CLas biology is critical for HLB management.

Enhancing PCR Capacity To Detect ' Liberibacter asiaticus' Utilizing Whole Genome Sequence Information.

' Liberibacter asiaticus' (CLas) is an unculturable α-proteobacterium associated with citrus Huanglongbing (HLB; yellow shoot disease). PCR procedures that accurately confirm or exclude CLas infection in citrus tissue/Asian citrus psyllid (ACP) samples are critical for HLB management. When CLas was described in 1994, a 23-bp signature oligonucleotide sequence (OI1) in the 16S rRNA gene (, three genomic copies) was identified based on Sanger sequencing.

A Type 3 Prophage of 'Candidatus Liberibacter asiaticus' Carrying a Restriction-Modification System.

Prophages, the lysogenic form of bacterial phages, are important genetic entities of 'Candidatus Liberibacter asiaticus' (CLas), a nonculturable α-proteobacterium associated with citrus Huanglongbing. Two CLas prophages have been described, SC1 (NC_019549.1, Type 1) and SC2 (NC_019550.

Genome-wide association studies using a penalized moving-window regression.

Motivation: Genome-wide association studies (GWAS) have played an important role in identifying genetic variants underlying human complex traits. However, its success is hindered by weak effect at causal variants and presence of noise at non-causal variants. In an effort to overcome these difficulties, a previous study proposed a regularized regression method that penalizes on the difference of signal strength between two consecutive single-nucleotide polymorphisms (SNPs).

Unusual Five Copies and Dual Forms of nrdB in "Candidatus Liberibacter asiaticus": Biological Implications and PCR Detection Application.

"Candidatus Liberibacter asiaticus" (CLas), a non-culturable α-proteobacterium, is associated with citrus Huanglongbing (HLB, yellow shoot disease) currently threatening citrus production worldwide. Here, the whole genome sequence of CLas strain A4 from Guangdong of China was analyzed. Five copies of nrdB, encoding β-subunit of ribonucleotide reductase (RNR), a critical enzyme involving bacterial proliferation, were found.

Predominance of Single Prophage Carrying a CRISPR/cas System in "Candidatus Liberibacter asiaticus" Strains in Southern China.

"Candidatus Liberibacter asiaticus" (CLas) is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature.