Identification of a cellular role of hemolysin co-regulatory protein (Hcp) in Vibrio alginolyticus modulating substrate metabolism and biofilm formation by cAMP-CRP.

Cyclic AMP (cAMP) and cAMP receptor protein (CRP) system controls catabolic enzyme expression based on metabolite concentrations in bacteria. Hemolysin co-regulatory protein (Hcp) is well known as a molecular chaperone for virulence factor secretion of the type VI secretion system (T6SS). However, the intracellular role of Hcp involving in bacterial physiological processes remains unknown.

Comparison of visual acuity outcome and choroidal thickness variation of intravitreal ranibizumab injection for myopic choroidal neovascularization with or without dome-shaped macula.

Background: To investigate the visual acuity outcome and choroid thickness (CT) change after intravitreal ranibizumab in highly myopic eyes with or without dome-shaped macula (DSM) in Chinese patients.

Methods: This retrospective, observative study included 80 treatment-naive eyes (80 patients), which received ranibizumab according to the 1+PRN protocol. The best corrective visual acuity (BCVA) and CT change were compared between eyes with or without DSM.

Characteristics of the Peripapillary Structure and Vasculature in Patients With Myopic Anisometropia.

Purpose: To evaluate the interocular differences of the peripapillary structural and vascular parameters and that of association with axial length (AL) in participants with myopic anisometropia using swept-source optical coherence tomography.

Methods: This prospective cross-sectional study included 90 eyes of 45 participants. Each participant's eyes were divided into the more and less myopic eye respectively according to spherical equivalent.

A vault-prediction formula for implantable collamer lens based on preoperative parameters: a retrospective clinical study.

Objectives: The aim of the present study was to predict the vault of implantable collamer lens (ICL) using a formula established based on the preoperative parameters.

Methods: This retrospective study included data on 226 eyes from 114 patients(the median age and range was 27.5 ± 5.

Development and validation of a new multivariable prediction model to estimate risk of abnormal vault.

Purpose: To develop and validate a new multivariable prediction model to estimate risk of abnormal vault after EVO Implantable Collamer Lens (EVO-ICL) implantation using the preoperative parameters.

Methods: This retrospective study comprised 282 eyes of 143patients who underwent EVO-ICL surgery between May 2021 and April 2022. We measured preoperative parameters before surgery and vaults in 1 week after the operation using swept-source optical coherence tomography (SS-OCT).

Short-term postoperative changes in the choroidal vascularity index in patients with a unilateral epiretinal membrane.

Background: To investigate short-term choroidal structural and vascular changes after epiretinal membrane (ERM) surgery.

Methods: In this retrospective study, 65 patients with unilateral ERM underwent pars plana vitrectomy combined with cataract surgery and were examined one day before surgery and one week, one month, and three months after surgery. Choroidal thickness (CT) and choroidal vascular index (CVI) were evaluated using horizontal enhanced depth imaging optical coherence tomography (EDI-OCT) scans and were further calculated using semi-automatic algorithms using MATLAB R2017a.

Rapid prototyping enzyme homologs to improve titer of nicotinamide mononucleotide using a strategy combining cell-free protein synthesis with split GFP.

Engineering biological systems to test new pathway variants containing different enzyme homologs is laborious and time-consuming. To tackle this challenge, a strategy was developed for rapidly prototyping enzyme homologs by combining cell-free protein synthesis (CFPS) with split green fluorescent protein (GFP). This strategy featured two main advantages: (1) dozens of enzyme homologs were parallelly produced by CFPS within hours, and (2) the expression level and activity of each homolog was determined simultaneously by using the split GFP assay.

The effectiveness and safety of the rapid titration strategy of background controlled-release oxycodone hydrochloride for patients with moderate-to-severe cancer pain: A retrospective cohort study.

Background: Oxycodone hydrochloride is a semisynthetic narcotic analgesic agent. This study aimed to explore optimal titration strategy of controlled-release (CR) oxycodone hydrochloride in patients with cancer pain.

Methods: 258 patients, who used regular strong opioids (morphine and CR oxycodone hydrochloride) for cancer pain across 25 three grade class hospitals in China during January 15th 2017 to April 30th 2017, were retrospectively studied.

Medial tibial plateau sustaining higher physiological stress than the lateral plateau: based on 3D printing and finite element method.

Background: Medial compartment knee osteoarthritis (KOA) accounts for most KOA cases, and increased trabecular bone volume fraction (BV/TV) is one of the pathological changes in the tibial plateau of KOA. How BV/TV changes before and after the menopause and its effects on medial compartment KOA are yet to be clarified.

Methods: Twenty femurs from twenty 12-week-old rats were included.

Construction of luminescent Escherichia coli via expressing lux operons and their application on toxicity test.

Recombinant luminescent Escherichia coli strains could be used to detect the toxicity of pure or mixed contaminants as a light-off sensor. In this work, the lux operon of Photobacterium phosphoreum T3 was identified for the first time. Recombinant luminescent E.

Effects of Exercise on Optic Nerve and Macular Perfusion in Glaucoma and Normal Subjects.

Prcis: Using optical coherence tomography angiography, we found an increase in the blood flow in regional macular without significant changes in the optic disk in patients with glaucoma after a moderate exercise regime.

Purpose: To investigate the effect of moderate exercise on optic nerve and macular perfusion in glaucoma patients.

Methods: Overall, 25 eyes of 25 patients with primary open angle glaucoma and 22 eyes of 22 normal subjects were included in the glaucoma and control groups, respectively.

Characteristics of the Optic Nerve Head in Myopic Eyes Using Swept-Source Optical Coherence Tomography.

Purpose: To investigate the characteristics of the optic nerve head (ONH) in myopia using swept-source optical coherence tomography (SS-OCT).

Methods: Participants were divided into three groups according to the axial length (AL). The optic disc morphology, retinal nerve fiber layer (RNFL) thickness, and radial peripapillary capillary (RPC) vessel density (VD), optic disc tilt, rotation, Bruch's membrane opening distance (BMOD), border length (BL), border tissue angle, focal lamina cribrosa (LC) defects, β- and γ-zone peripapillary atrophy (PPA), microvasculature dropout (MvD), choroidal thickness (CT), and the choroidal vascularity index (CVI) were compared.

Choroidal Vascularity Index Changes After Exercise in Patients With Glaucoma.

To investigate the changes in choroidal vascular structures after exercise in patients with glaucoma using an image binarisation algorithm with enhanced-depth imaging optical coherence tomography. Thirty-four eyes of 19 patients with primary open-angle glaucoma and 40 eyes of 20 normal subjects were included in the glaucoma and control groups, respectively. All subjects were evaluated before, immediately after, and 30 min after 20-min running at moderate speed.

Characteristics of macular microvasculature before and after idiopathic macular hole surgery.

Aim: To evaluate the macular microvasculature before and after surgery for idiopathic macular hole (MH) and the association of preoperative vascular parameters with postoperative recovery of visual acuity and configuration.

Methods: Twenty eyes from 20 patients with idiopathic MH were enrolled. Optical coherence tomography angiography (OCTA) images were obtained before, 2wk, 1, and 3mo after vitrectomy with internal limiting membrane peeling.

Anlotinib Monotherapy for Refractory Metastatic Colorectal Cancer: A Double-Blinded, Placebo-Controlled, Randomized Phase III Trial (ALTER0703).

Background: Treatment options for refractory metastatic colorectal cancer (mCRC) were limited. Anlotinib is a novel multitarget tyrosine kinase inhibitor. ALTER0703 study was conducted to assess efficacy and safety of anlotinib for patients with refractory mCRC.

Transition element doped octahedral manganese molecular sieves (Me-OMS-2) as diclofenac adsorbents.

Diclofenac (DCF) control measures have become an area of increased interest for environmental researchers due to the high environmental concentration and risk of DCF. Adsorption seems to be promising for DCF removal from the aqueous phase because of its specific superiority in comparison with biodegradation, membrane separation, and advanced oxidation or reduction. In this study, OMS-2 and metal-doped OMS-2 ((Me-OMS-2, with Me = Co, Cu or Ce) were prepared and tested as adsorbents for the removal of DCF.

Binuclear cobalt phthalocyanine supported on manganese octahedral molecular sieve: High-efficiency catalyzer of peroxymonosulfate decomposition for degrading propranolol.

Propranolol (PRO) is widely detected in the aquatic environment and proved to be detrimental to multifarious aquatic organisms. In view of some virtues of sulfate radicals than hydroxyl radicals, advanced oxidation technologies that involve the activation of peroxymonosulfate (PMS) have stimulated wide-ranging research on the PRO removal. In this paper, a composite (CNOMS-2) of amino-functionalized manganese octahedral molecular sieve (NOMS-2) and binuclear cobalt phthalocyanine (CoCPc) was synthesized easily, and utilized as a catalyzer for PMS to degrade PRO in water.

Cobalt tetracarboxyl phthalocyanine-manganese octahedral molecular sieve (OMS-2) as a heterogeneous catalyst of peroxymonosulfate for degradation of diclofenac.

A composite of cobalt tetracarboxyl phthalocyanine and amino-functionalized manganese octahedral molecular sieve (CNOMS-2) was synthesized in a simple way, and applied for degrading diclofenac in aqueous media by catalyzing of peroxymonosulfate heterogeneously. The experiment results revealed that the CNOMS-2/PMS system was highly efficient for DCF degradation in the pH range of 5-9, and the mechanism involved the generation of OH, SO⁻ and O by the activation of PMS. The main reactive oxygen species was found to be O by radical scavenging experiments and electron paramagnetic resonance (EPR) spectroscopy.

Erythrocyte concentrates recovered from under-collected whole blood: experimental and clinical results.

Background: Although periodic blood shortages are widespread in major Chinese cities, approximately 1 x 10(5) U of whole blood are discarded yearly because of under-collection. To reduce the wastage of acid citrate dextrose solution B (ACD-B) anticoagulated under-collected whole blood (UC-WB), this study was performed to elucidate the effect of extracellular pH and holding time on erythrocyte quality. Mannitol-adenine-phosphate (MAP) erythrocyte concentrates (UC-RBCs) were prepared with UC-WB to assess the safety and efficacy of this component.