Publications by authors named "Minhui Wu"

Cyclic AMP (cAMP) and cAMP receptor protein (CRP) system controls catabolic enzyme expression based on metabolite concentrations in bacteria. Hemolysin co-regulatory protein (Hcp) is well known as a molecular chaperone for virulence factor secretion of the type VI secretion system (T6SS). However, the intracellular role of Hcp involving in bacterial physiological processes remains unknown.

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Background: To investigate the visual acuity outcome and choroid thickness (CT) change after intravitreal ranibizumab in highly myopic eyes with or without dome-shaped macula (DSM) in Chinese patients.

Methods: This retrospective, observative study included 80 treatment-naive eyes (80 patients), which received ranibizumab according to the 1+PRN protocol. The best corrective visual acuity (BCVA) and CT change were compared between eyes with or without DSM.

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Article Synopsis
  • - This study aimed to assess the effectiveness of two different photodynamic therapy (PDT) protocols combined with the drug bevacizumab for treating corneal neovascularization (CNV) in patients unresponsive to conventional treatments.
  • - Patients were divided into two groups: one received lower energy PDT parameters, while the other received higher energy parameters, with the bevacizumab injection given immediately after PDT.
  • - Results showed that overall, 60% of patients experienced improved vision and there was a significant reduction in neovascularization, particularly in the group that received the higher energy treatment, demonstrating the potential of optimized combined therapy for CNV.
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Purpose: To evaluate the interocular differences of the peripapillary structural and vascular parameters and that of association with axial length (AL) in participants with myopic anisometropia using swept-source optical coherence tomography.

Methods: This prospective cross-sectional study included 90 eyes of 45 participants. Each participant's eyes were divided into the more and less myopic eye respectively according to spherical equivalent.

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Objectives: The aim of the present study was to predict the vault of implantable collamer lens (ICL) using a formula established based on the preoperative parameters.

Methods: This retrospective study included data on 226 eyes from 114 patients(the median age and range was 27.5 ± 5.

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Purpose: To develop and validate a new multivariable prediction model to estimate risk of abnormal vault after EVO Implantable Collamer Lens (EVO-ICL) implantation using the preoperative parameters.

Methods: This retrospective study comprised 282 eyes of 143patients who underwent EVO-ICL surgery between May 2021 and April 2022. We measured preoperative parameters before surgery and vaults in 1 week after the operation using swept-source optical coherence tomography (SS-OCT).

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Background: To investigate short-term choroidal structural and vascular changes after epiretinal membrane (ERM) surgery.

Methods: In this retrospective study, 65 patients with unilateral ERM underwent pars plana vitrectomy combined with cataract surgery and were examined one day before surgery and one week, one month, and three months after surgery. Choroidal thickness (CT) and choroidal vascular index (CVI) were evaluated using horizontal enhanced depth imaging optical coherence tomography (EDI-OCT) scans and were further calculated using semi-automatic algorithms using MATLAB R2017a.

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Engineering biological systems to test new pathway variants containing different enzyme homologs is laborious and time-consuming. To tackle this challenge, a strategy was developed for rapidly prototyping enzyme homologs by combining cell-free protein synthesis (CFPS) with split green fluorescent protein (GFP). This strategy featured two main advantages: (1) dozens of enzyme homologs were parallelly produced by CFPS within hours, and (2) the expression level and activity of each homolog was determined simultaneously by using the split GFP assay.

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Article Synopsis
  • - This article is a systematic review highlighting the significance of intelligent hydrogels in biomedical engineering due to their biocompatibility, rapid response, and similarity to living tissue.
  • - It compares traditional hydrogels with smart hydrogels, discusses various types, their mechanisms, materials used for creation, and different morphologies like films and fibers.
  • - The review also addresses current research limitations and explores future prospects, aiming to inspire researchers in the field for further development of smart hydrogels.
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Background: Oxycodone hydrochloride is a semisynthetic narcotic analgesic agent. This study aimed to explore optimal titration strategy of controlled-release (CR) oxycodone hydrochloride in patients with cancer pain.

Methods: 258 patients, who used regular strong opioids (morphine and CR oxycodone hydrochloride) for cancer pain across 25 three grade class hospitals in China during January 15th 2017 to April 30th 2017, were retrospectively studied.

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Background: Medial compartment knee osteoarthritis (KOA) accounts for most KOA cases, and increased trabecular bone volume fraction (BV/TV) is one of the pathological changes in the tibial plateau of KOA. How BV/TV changes before and after the menopause and its effects on medial compartment KOA are yet to be clarified.

Methods: Twenty femurs from twenty 12-week-old rats were included.

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  • - This paper reviews sustained-release drug-loaded microspheres, highlighting their long-lasting delivery capabilities and effectiveness in targeted therapies, particularly within tissue engineering applications.
  • - It discusses various types of microspheres, their preparation methods, and required materials, showcasing the ease of their production and versatility in research and medical fields.
  • - The paper also addresses the current limitations of these drug delivery systems and explores future opportunities for improvement and innovation in the development of sustained-release drug microspheres.
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Recombinant luminescent Escherichia coli strains could be used to detect the toxicity of pure or mixed contaminants as a light-off sensor. In this work, the lux operon of Photobacterium phosphoreum T3 was identified for the first time. Recombinant luminescent E.

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Article Synopsis
  • Porcine circovirus type 4 (PCV4) is a new virus linked to a disease in pigs called porcine dermatitis and nephropathy syndrome (PDNS), first detected in China in 2019.
  • A study of 42 related PCV4 sequences found that they can be classified into two subgenotypes: PCV4a (the main strain) and PCV4b, with further divisions in the PCV4a group.
  • The research shows high genetic similarity among the strains and identifies key variable regions in the virus's proteins, which can help inform strategies for controlling its spread in China.
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Prcis: Using optical coherence tomography angiography, we found an increase in the blood flow in regional macular without significant changes in the optic disk in patients with glaucoma after a moderate exercise regime.

Purpose: To investigate the effect of moderate exercise on optic nerve and macular perfusion in glaucoma patients.

Methods: Overall, 25 eyes of 25 patients with primary open angle glaucoma and 22 eyes of 22 normal subjects were included in the glaucoma and control groups, respectively.

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Purpose: To investigate the characteristics of the optic nerve head (ONH) in myopia using swept-source optical coherence tomography (SS-OCT).

Methods: Participants were divided into three groups according to the axial length (AL). The optic disc morphology, retinal nerve fiber layer (RNFL) thickness, and radial peripapillary capillary (RPC) vessel density (VD), optic disc tilt, rotation, Bruch's membrane opening distance (BMOD), border length (BL), border tissue angle, focal lamina cribrosa (LC) defects, β- and γ-zone peripapillary atrophy (PPA), microvasculature dropout (MvD), choroidal thickness (CT), and the choroidal vascularity index (CVI) were compared.

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To investigate the changes in choroidal vascular structures after exercise in patients with glaucoma using an image binarisation algorithm with enhanced-depth imaging optical coherence tomography. Thirty-four eyes of 19 patients with primary open-angle glaucoma and 40 eyes of 20 normal subjects were included in the glaucoma and control groups, respectively. All subjects were evaluated before, immediately after, and 30 min after 20-min running at moderate speed.

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Aim: To evaluate the macular microvasculature before and after surgery for idiopathic macular hole (MH) and the association of preoperative vascular parameters with postoperative recovery of visual acuity and configuration.

Methods: Twenty eyes from 20 patients with idiopathic MH were enrolled. Optical coherence tomography angiography (OCTA) images were obtained before, 2wk, 1, and 3mo after vitrectomy with internal limiting membrane peeling.

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Background: Treatment options for refractory metastatic colorectal cancer (mCRC) were limited. Anlotinib is a novel multitarget tyrosine kinase inhibitor. ALTER0703 study was conducted to assess efficacy and safety of anlotinib for patients with refractory mCRC.

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Diclofenac (DCF) control measures have become an area of increased interest for environmental researchers due to the high environmental concentration and risk of DCF. Adsorption seems to be promising for DCF removal from the aqueous phase because of its specific superiority in comparison with biodegradation, membrane separation, and advanced oxidation or reduction. In this study, OMS-2 and metal-doped OMS-2 ((Me-OMS-2, with Me = Co, Cu or Ce) were prepared and tested as adsorbents for the removal of DCF.

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  • The study explored the differences in volatile organic compounds (VOCs) from the feces of rats treated with Baihe Jizihuang Tang (BHT) in a depression model induced by chronic stress, using advanced analysis techniques.
  • Eleven specific biomarkers were identified through various statistical methods, showcasing the potential therapeutic effects of traditional Chinese medicine (TCM) in treating complex diseases.
  • The research demonstrated the effectiveness of using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) for quickly identifying clinical biomarkers without extensive sample preparation, supporting its application in future studies.
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Propranolol (PRO) is widely detected in the aquatic environment and proved to be detrimental to multifarious aquatic organisms. In view of some virtues of sulfate radicals than hydroxyl radicals, advanced oxidation technologies that involve the activation of peroxymonosulfate (PMS) have stimulated wide-ranging research on the PRO removal. In this paper, a composite (CNOMS-2) of amino-functionalized manganese octahedral molecular sieve (NOMS-2) and binuclear cobalt phthalocyanine (CoCPc) was synthesized easily, and utilized as a catalyzer for PMS to degrade PRO in water.

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A composite of cobalt tetracarboxyl phthalocyanine and amino-functionalized manganese octahedral molecular sieve (CNOMS-2) was synthesized in a simple way, and applied for degrading diclofenac in aqueous media by catalyzing of peroxymonosulfate heterogeneously. The experiment results revealed that the CNOMS-2/PMS system was highly efficient for DCF degradation in the pH range of 5-9, and the mechanism involved the generation of OH, SO⁻ and O by the activation of PMS. The main reactive oxygen species was found to be O by radical scavenging experiments and electron paramagnetic resonance (EPR) spectroscopy.

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  • - The study aimed to understand the cause of an ABO blood group discrepancy in a patient using genotyping and to determine an appropriate blood transfusion strategy.
  • - Results showed that the patient had conflicting ABO typing, with forward typing indicating B blood type and reverse typing showing AB subtype, confirmed by tests indicating weak A antigens.
  • - Genetic analysis revealed a specific mutation in the ABO gene responsible for decreased A antigen expression, identifying the patient's blood group genotype as ABO*Ael05/B101.
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Background: Although periodic blood shortages are widespread in major Chinese cities, approximately 1 x 10(5) U of whole blood are discarded yearly because of under-collection. To reduce the wastage of acid citrate dextrose solution B (ACD-B) anticoagulated under-collected whole blood (UC-WB), this study was performed to elucidate the effect of extracellular pH and holding time on erythrocyte quality. Mannitol-adenine-phosphate (MAP) erythrocyte concentrates (UC-RBCs) were prepared with UC-WB to assess the safety and efficacy of this component.

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