Novel melanin-derived stationary phase for immobilized metal ion affinity chromatography in recombinant His-tagged protein purification.

The matrix of the stationary phase is a crucial element in affinity chromatography for protein purification. Various materials, including polymer or magnetic materials, have been employed as the matrix in the purification of His-tagged protein. Here, for the first time, we utilized a combination of melanin and alginate, both natural polymer materials, to synthesize Ni-melanin/alginate (Ni-M/A) beads for His-tagged protein purification.

The Golgi-localized transporter OsPML4 contributes to manganese homeostasis in rice.

Manganese (Mn), an indispensable plant micronutrient, functions as a vital enzyme co-factor in numerous biochemical reactions. In rice, the Golgi-localized PHOTOSYNTHESIS-AFFECTED MUTANT 71-LIKE 3 (OsPML3), a member of the UNCHARACTERIZED PROTEIN FAMILY (UPF0016), plays a pivotal role in Mn homeostasis, particularly in rapidly developing tissues. This study focused on the functional characterization of another UPF0016 family member in rice, OsPML4, to elucidate its involvement in Mn homeostasis.

A portable ascorbic acid in sweat analysis system based on highly crystalline conductive nickel-based metal-organic framework (Ni-MOF).

Conductive metal-organic frameworks can provide unique porous structures, large pore volumes, many catalytically active sites and high crystallinity, and so are becoming increasingly important and attractive as electrocatalytic materials. The present work synthesized nanorods of the conductive compound Ni(2,3,6,7,10,11-hexaiminotriphenylene) (Ni(HITP)) with a high degree of crystallinity from HITP ligands and Ni ions. Screen-printed electrodes made with this material were employed to fabricate an enzyme-free sensor for the detection of ascorbic acid (AA).

Selective oxidation of cyclohexane on a novel catalyst Mg-Cu/SBA-15 by molecular oxygen.

The novel catalysts xMg-2.3Cu/SBA-15 with copper and magnesium oxide co-supported on mesoporous silica were synthesized by an impregnation method. The newly synthesized catalysts were characterized using a series of techniques such as BET, XRD, H2-TPR, UV-vis, XPS, EDS and TEM.