Enhanced carbonate weathering and CO release in a typical karst watershed (Southwest China): Evidence from hydrochemical and multi-isotopic data.

Carbon sinks formed by carbonate weathering are a major component of the carbon sink deficit and play a significant role in the carbon cycle. However, exogenous acids (HNO and HSO) are also involved in carbonate weathering, complicating the overestimation of the formation of carbon sinks and fluxes. In this study, we analyzed the Hongjiadu karst underground river basin in Guizhou using isotopes (δC-DIC, δS-SO, δO-SO, δO-HO, and δH-HO) and hydrochemical methods to investigate carbon cycling characteristics and its influencing factors, and quantitatively evaluate the effects of HNO + HSO on CO sink fluxes in the basin.

Preparation of phenol-formaldehyde composite modified with chitosan for the simultaneous removal of antibiotics and heavy metal ions in waters.

In current study, a new adsorbent based on aminated phenol-formaldehyde composite was prepared using chitosan as modifier. Various techniques were adopted to characterize the morphology and structure of the prepared adsorbent. Due to the abundant amino groups, the obtained adsorbent presented satisfactory adsorption performance towards fluoroquinolones (FQs) and heavy metal ions (including Cu, Cd and Pb) by means of multiple forces including electrostatic, H-bonding, π-π stacking interactions (for FQs) and chelating force (for heavy metal ions).

Tracing contaminants of emerging concern and their transformations in the whole treatment process of a municipal wastewater treatment plant using nontarget screening and molecular networking strategies.

This study employed nontarget screening with high-resolution mass spectrometry and molecular network strategy to characterize the occurrence and tranformation of contaminants of emerging concern (CECs) through a wastewater treatment plant in Guangzhou. We detected 70,631 compounds in positive mode and 14,423 in negative mode in influent, from which 94.5 % of these compounds were successfully eliminated after treatment.

Occurrence and fate of five representative neonicotinoid insecticides across different wastewater treatment plants and the impact on receiving water bodies.

Neonicotinoids (NEOs), despite their widespread use as insecticides, exhibit a notable knowledge deficit in regards to their presence in wastewater treatment plants (WWTPs) and their surrounding environments. This study delves into the presence and disposition of 5 NEOs: Thiamethoxam (THM), Clothianidin (CLO), Imidacloprid (IMD), Acetamiprid (ACE), and Thiacloprid (THA) across 3 domestic WWTPs and their receiving waters. Notably, THM, CLO, and ACE were consistently detected in all water and sludge samples, with THM emerging as the most abundant compound in both influent and effluent.

Ferric citrate enhanced bioreduction of Cr(VI) by Bacillus cereus RCr in aqueous solutions: reduction performance and mechanisms.

The bioreduction characteristics and mechanisms of Cr(VI) onto Bacillus cereus RCr enhanced by ferric citrate were investigated. The optimum conditions were initial pH 9, temperature 40 °C, inoculation amount 4%, and glucose 3 g/L, respectively. The addition of 1.

Trash into Treasure: Nano-coating of Catheter Utilizes Urine to Deprive HS Against Persister and Rip Biofilm Matrix.

Bacteria-derived hydrogen sulfide (HS) often contributes to the emergence of antibiotic-recalcitrant bacteria, especially persister (a sub-population of dormant bacteria), thus causing the treatment failure of Catheter-associated urinary tract infection (CAUTI). Here, an HS harvester nanosystem to prevent the generation of persister bacteria and disrupt the dense biofilm matrix by the self-adaptive ability of shape-morphing is prepared. The nanosystem possesses a core-shell structure that is composed of liquid metal nanoparticle (LM NP), AgNPs, and immobilized urease.

Malassezia restricta as an unexpected cause of infectious osteomyelitis diagnosed by metagenomic sequencing: a case report and literature review.

Background: Malassezia restricta, a lipophilic and lipodependent yeast belonging to the basidiomycetes group, is an opportunistic fungal pathogen associated with various skin diseases, including seborrheic dermatitis and dandruff. Typically, Malassezia infection in neonates manifests as fungemia or hematogenous dissemination to the bone or lungs. However, vertebral osteomyelitis caused by these fungi is rarely reported owing to non-specific clinical presentations and laboratory/imaging findings.

Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design-Build-Test-Learn approach.

Industrial production of bioactive compounds from actinobacteria, such as erythromycin and its derivatives, faces challenges in achieving optimal yields. To this end, the Design-Build-Test-Learn (DBTL) framework, a systematic metabolic engineering approach, was employed to enhance erythromycin production in Saccharopolyspora erythraea (S. erythraea) E3 strain.

Incidence and risk factors for potential drug-drug interactions in outpatients receiving opioid analgesics.

Background: Opioids are the most frequently used drugs to treat pain in cancer patients. However opioid analgesics can cause adverse effects and potential drug-drug interaction.

Research Design And Methods: This cross-sectional retrospective study analyzed pDDI in 1839 patients with opioid analgesics in a large comprehensive hospital in China from January 1 to 31 December 2022.

Response mechanism of a highly efficient partial nitritation-anammox (PN/A) process under antibiotic stress: Extracellular polymers, microbial community, and functional genes.

The Partial nitritation-Anammox (PN/A) process can be restricted when treating high ammonia nitrogen wastewater containing antibiotics. This study aims to explore the response mechanism of the PN/A process under antibiotic stress. Results showed the PN/A process achieved a nitrogen removal rate higher than 1.

Towards a hybrid model-driven platform based on flux balance analysis and a machine learning pipeline for biosystem design.

Metabolic modeling and machine learning (ML) are crucial components of the evolving next-generation tools in systems and synthetic biology, aiming to unravel the intricate relationship between genotype, phenotype, and the environment. Nonetheless, the comprehensive exploration of integrating these two frameworks, and fully harnessing the potential of fluxomic data, remains an unexplored territory. In this study, we present, rigorously evaluate, and compare ML-based techniques for data integration.

Linking PAHs concentration, risk to PAHs source shift in soil and water in epikarst spring systems, Southwest China.

A systematic assessment of the variations in the ecological risk of PAHs and the key emission sources controlling the variations is of great importance to human health and aquatic organisms. PAH concentrations, composition, source, and ecological risk in soils and water in two different periods (2010-2011 and 2019) of three typical epikarst springs in Southwest China were investigated. Results showed that PAH concentrations in soil and water have an overall downward trend (a reduction of 57 % and 93 %, respectively) in the past 10 years, which is consistent with the downward trend in the relative contribution rate of raw coal production (a 66 % reduction).

Thiamethoxam adsorption by ZnCl modified cow manure biochar: Mechanism and quantitative prediction.

The overuse of thiamethoxam (THM) has threatened the survival of living organisms and it is necessary to find an environmentally friendly material to remove THM frequently detected in water. Biochar prepared from cow manure modified with ZnCl (Zn-CBC) was used to remove THM. Compared to the unmodified cow manure biochar (CBC), the removal ratio of THM by Zn-CBC was enhanced 35 times.

Free nitrous acid prediction in ANAMMOX process using hybrid deep neural network model.

Free nitrous acid (FNA) is a critical metric for stabilization of ANAMMOX but can not be directly and immediately measured by sensors or chemical measurement method, which hinders the effective management and operation for ANAMMOX. This study focuses on FNA prediction using hybrid model based on temporal convolutional network (TCN) combined with attention mechanism (AM) optimized by multiobjective tree-structured parzen estimator (MOTPE), called MOTPE-TCNA. A case study in an ANAMMOX reactor is carried out.

Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora erythraea.

In this study, the cellular metabolic mechanisms regarding ammonium sulfate supplementation on erythromycin production were investigated by employing targeted metabolomics and metabolic flux analysis. The results suggested that the addition of ammonium sulfate stimulates erythromycin biosynthesis. Targeted metabolomics analysis uncovered that the addition of ammonium sulfate during the late stage of fermentation resulted in an augmented intracellular amino acid metabolism pool, guaranteeing an ample supply of precursors for organic acids and coenzyme A-related compounds.

Accurate multi-objective prediction of CO emission performance indexes and industrial structure optimization using multihead attention-based convolutional neural network.

The establishment of specific targets for the global carbon peaking and neutrality raises urgent requirements for prediction of CO emission performance indexes (CEPIs) and industrial structure optimization. However, accurate multi-objective prediction of CEPIs is still a knotty problem. In the present study, multihead attention-based convolutional neural network (MHA-CNN) model was proposed for accurate prediction of 4 CEPIs and further provided the rational suggestions for further industrial structure optimization.

Novel hydrophilic straw biochar for the adsorption of neonicotinoids: kinetics, thermodynamics, influencing factors, and reuse performance.

Nitenpyram (NIT) is the most water-soluble neonicotinoid (NEO). It has been shown to pose a serious threat to human health and the environment but was always ignored due to its limited market share. There were few experts who studied NIT's transport behavior on biochar.

Enhanced biodegradation of thiamethoxam with a novel polyvinyl alcohol (PVA)/sodium alginate (SA)/biochar immobilized Chryseobacterium sp H5.

Long-term and extensive usage of thiamethoxam, the second-generation neonicotinoid insecticide, has caused a serious threat to non-target organisms and ecological security. Efficient immobilized microorganism techniques are a sustainable solution for bioremediation of thiamethoxam contamination. A Gram-negative aerobic bacterium Chryseobacterium sp H5 with high thiamethoxam-degrading efficiencies was isolated from activated sludge.

Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring to promote osteogenesis via TAF15-RUNX2 pathway.

Effect of artesunate (ART)-treated bone marrow-derived mesenchymal stem cells-derived exosomes (BMSC-Exos) on osteogenesis and its underlying mechanisms were investigated. Proliferation, alkaline phosphatase activity and calcified nodule formation of osteoblasts were determined. A mouse model of osteoporosis was established by ovariectomy.

The neural stem cell properties of Pkd2l1 cerebrospinal fluid-contacting neurons .

The neural stem cells (NSCs) in the ventricular-subventricular zone of the adult mammalian spinal cord may be of great benefit for repairing spinal cord injuries. However, the sources of NSCs remain unclear. Previously, we have confirmed that cerebrospinal fluid-contacting neurons (CSF-cNs) have NSC potential .

Occurrence, variations, and risk assessment of neonicotinoid insecticides in Harbin section of the Songhua River, northeast China.

Neonicotinoid insecticides (NNIs) have been intensively used and exploited, resulting in their presence and accumulation in multiple environmental media. We herein investigated the current levels of eight major NNIs in the Harbin section of the Songhua River in northeast China, providing the first systematic report on NNIs in this region. At least four NNIs in water and three in sediment were detected, with total concentrations ranging from 30.

The Shift of Soil Bacterial Community After Afforestation Influence Soil Organic Carbon and Aggregate Stability in Karst Region.

Soil microbes regulate the carbon cycle and affect the formation and stabilization of soil aggregates. However, the interactions between the soil microbial community and soil organic carbon (SOC) fractions, organic carbon (OC) content in aggregates, and soil aggregate stability after afforestation are remain poorly understood. In our study, we investigated SOC fractions in bulk soil, aggregate-associated OC content, soil aggregate stability, and soil bacterial community with high-throughput 16S rRNA sequencing at sites representing natural secondary forest (NF) and managed forest (MF), with cropland (CL) as reference in a degraded karst region of Southwest China.

Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production.

As a novel protein post-translational modification (PTM), lysine succinylation is widely involved in metabolism regulation by altering the activity of catalytic enzymes. Inactivating succinyl-CoA synthetase in Saccharopolyspora erythraea HL3168 E3 was proved significantly inducing the global protein hypersuccinylation. To investigate the effects, succinylome of the mutant strain E3ΔsucC was identified by using a high-resolution mass spectrometry-based proteomics approach.

Reconstruction of the Genome-Scale Metabolic Model of and Its Application in the Overproduction of Erythromycin.

is considered to be an effective host for erythromycin. However, little is known about the regulation in terms of its metabolism. To develop an accurate model-driven strategy for the efficient production of erythromycin, a genome-scale metabolic model (iJL1426) was reconstructed for the industrial strain.

Detection of Neonicotinoids in agriculture soil and degradation of thiacloprid through photo degradation, biodegradation and photo-biodegradation.

The social and ecological influence of Neonicotinoids (NEOs) usage in agriculture sector is progressively higher. There are seven NEOs insecticides widely used for the insects control. Among the NEOs, thiacloprid (THD) was extensively used for insect control during crop cultivation.