Structural basis for the asymmetric binding of coactivator SRC1 to FXR-RXRα and allosteric communication within the complex.

Farnesoid X receptor (FXR) is a promising target for treatment of metabolic associated fatty liver disease (MAFLD). In this study, we employed an integrative approach to investigate the interaction between FXR-RXRα-DNA complex and the entire coactivator SRC1-NRID (nuclear receptor interaction domain). We constructed a multi-domain model of FXR-RXRα-DNA, highlighting the interface between FXR-DBD and LBD.

Single-cell RNA-seq reveals the immune response of Co-infection with streptococcus pneumoniae after influenza A virus by a lung-on-chip: The molecular structure and mechanism of tight junction protein ZO-1.

Secondary bacterial infection is the main cause of pneumonia after influenza virus infection. This study aims to explore the impact of co-infection of influenza A virus (IAV) and Streptococcus pneumoniae (SP) on lung immune response using a human lung-on-chip model and single-cell RNA sequencing technology, with a focus on the molecular structure and mechanism of action of the tight junction protein ZO-1 in this process. Research and construct a human lung-on-chip model to simulate the microenvironment of the lungs in vivo.

Estradiol Enhances Cerebellar Molecular Layer Interneuron-Purkinje Cell Synaptic Transmission and Improves Motor Learning Through ER-β in Vivo in Mice.

In the cerebellar cortex, 17β-estradiol (E2) binds to estrogen receptors (ERs) and plays a role in regulating cerebellar synaptic plasticity and motor learning behaviors. However, the underlying mechanisms remain unclear. In this study, we investigated the effects of E2 on synaptic transmission between cerebellar molecular layer interneurons (MLIs) and Purkinje cells (PCs) in urethane-anesthetized mice.

Fast perceptual learning induces location-specific facilitation and suppression at early stages of visual cortical processing.

Tens of minutes of training can significantly improve visual discriminability of human adults, and this fast perceptual learning (PL) effect is usually specific to the trained location, with little transfer to untrained locations. Although location specificity is generally considered as a hallmark of visual PL, it remains unclear whether it involves both facilitation of trained locations and suppression of untrained locations. Here we developed a novel experimental design to investigate the cognitive neural mechanism of location specificity of fast PL.

Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age.

A comprehensive understanding of the evolution of the immune landscape in humans across the entire lifespan at single-cell transcriptional and protein levels, during development, maturation and senescence is currently lacking. We recruited a total of 220 healthy volunteers from the Shanghai Pudong Cohort (NCT05206643), spanning 13 age groups from 0 to over 90 years, and profiled their peripheral immune cells through single-cell RNA-sequencing coupled with single T cell and B cell receptor sequencing, high-throughput mass cytometry, bulk RNA-sequencing and flow cytometry validation experiments. We revealed that T cells were the most strongly affected by age and experienced the most intensive rewiring in cell-cell interactions during specific age.

Cardiovascular patches applied in congenital cardiac surgery: Current materials and prospects.

Congenital Heart Defects (CHDs) are the most common congenital anomalies, affecting between 4 and 75 per 1000 live births. Cardiovascular patches (CVPs) are frequently used as part of the surgical armamentarium to reconstruct cardiovascular structures to correct CHDs in pediatric patients. This review aims to evaluate the history of cardiovascular patches, currently available options, clinical applications, and important features of these patches.

Bidirectional associations between insomnia symptoms and eating disorders: A two-wave longitudinal study among Chinese college students.

Previous literature suggests a link between insomnia symptoms and eating disorders (EDs), yet empirical data on the temporal associations between these two variables are lacking. This study aimed to explore the bidirectional associations between insomnia symptoms and EDs in a population of college students. A total of 11,010 college students were assessed initially from October 17 to 29, 2023, and again 6 months later from April 15 to 24, 2024.

Polyethylene hydrogenolysis by dilute RuPt alloy to achieve H-pressure-independent low methane selectivity.

Chemical recycling of plastic waste could reduce its environmental impact and create a more sustainable society. Hydrogenolysis is a viable method for polyolefin valorization but typically requires high hydrogen pressures to minimize methane production. Here, we circumvent this stringent requirement using dilute RuPt alloy to suppress the undesired terminal C-C scission under hydrogen-lean conditions.

Review of Foam with Novel CO-Soluble Surfactants for Improved Mobility Control in Tight Oil Reservoirs.

CO-soluble surfactant foam systems have gained significant attention for their potential to enhance oil recovery, particularly in tight oil reservoirs where conventional water-soluble surfactants face challenges such as poor injectability and high reservoir sensitivity. This review provides a comprehensive explanation of the basic theory of CO-soluble surfactant foam, its mechanism in enhanced oil recovery (EOR), and the classification and application of various CO-soluble surfactants. The application of these surfactants in tight oil reservoirs, where low permeability and high water sensitivity limit traditional methods, is highlighted as a promising solution to improve CO mobility control and increase oil recovery.

Root morphology, nitrogen metabolism and amino acid metabolism in soybean under low phosphorus stress.

Phosphorus deficiency is a major influence on growth and development of soybean. Therefore, improving phosphorus utilization efficiency in soybean is a research priority for the soybean community. In this experiment, Liaodou 13 (high phosphorus utilization: HPE) and Tiefeng 3 (low phosphorus utilization: LPE) were used as test varieties.

Unleashing the strengths of unlabelled data in deep learning-assisted pan-cancer abdominal organ quantification: the FLARE22 challenge.

Deep learning has shown great potential to automate abdominal organ segmentation and quantification. However, most existing algorithms rely on expert annotations and do not have comprehensive evaluations in real-world multinational settings. To address these limitations, we organised the FLARE 2022 challenge to benchmark fast, low-resource, and accurate abdominal organ segmentation algorithms.

Fate of arsenic in contaminated coastal soil induced by rising temperature and seawater intrusion.

Temperature rising and seawater intrusion are expected to influence the hydrologic regime and redox conditions in coastal soil, and the fate and mechanisms of biogeochemical cycling of Arsenic (As) in the specific environment are poorly understood. This work was carried out in an anaerobic operating chamber by adding sulfate to simulate seawater intrusion under various temperature. Results demonstrated the microbial community diversity was influenced by temperature and the highest Shannon and lowest Simpson index were found at 28 °C.

Susceptibility to Attentional Capture by Target-Matching Distractors Predicts High Visual Working Memory Capacity.

Recent event-related potential (ERP) studies showed that individuals with low visual working memory (VWM) capacity are more susceptible to salience-driven attentional capture than high-capacity individuals are, with the latter being able to proactively suppress salient but irrelevant distractors. However, it remains unclear whether and how contingent attentional capture by distractors that possess a task-relevant (target) feature is related to VWM capacity. Here, we adopted a central focused-attention task that contained peripheral target-matching distractors to investigate this issue ( = 51 adults).

Prevalence of cyberbullying involvement and its association with clinical correlates among Chinese college students.

Background: Cyberbullying refers to a modern form of bullying that could be practiced electronically or on the Internet. This study conducted a cross-sectional survey to explore different types of cyberbullying involvement (CI) and the associations of CI with demographic characteristics and mental health problems among Chinese college students.

Methods: A total sample of 18,578 Chinese college students were recruited (68.

Estimation of invasive coronary perfusion pressure using electrocardiogram and Photoplethysmography in a porcine model of cardiac arrest.

Background: Coronary perfusion pressure (CPP) indicates spontaneous return of circulation and is recommended for high-quality cardiopulmonary resuscitation (CPR). This study aimed to investigate a method for non-invasive estimation of CPP using electrocardiography (ECG) and photoplethysmography (PPG) during CPR.

Methods: Nine pigs were used in this study.

The emerging predictive and prognostic role of HER2 in HER2-negative early breast cancer: a retrospective study.

Purpose: Many patients with early breast cancer (eBC) undergoing neoadjuvant chemotherapy do not achieve pathological complete response (pCR), which is a prognostic factor. We examined the role of HER2-low expression in predicting pCR and prognosis in HER2-negative eBC.

Methods: We evaluated patients with stage I-III HER2-negative BC, treated between 2013 and 2023 at The Royal Marsden NHS Foundation Trust, London.

Enhancing HO Electrosynthesis at Industrial-Relevant Current in Acidic Media on Diatomic Cobalt Sites.

Electrocatalytic synthesis of hydrogen peroxide (HO) in acidic media is an efficient and eco-friendly approach to produce inherently stable HO, but limited by the lack of selective and stable catalysts under industrial-relevant current densities. Herein, we report a diatomic cobalt catalyst for two-electron oxygen reduction to efficiently produce HO at 50-400 mA cm in acid. Electrode kinetics study shows a >95% selectivity for two-electron oxygen reduction on the diatomic cobalt sites.

Self-supporting noncovalent Choline Alginate/Tannic acid/Ag antibacterial films for strawberry preservation.

Packaging materials with peculiar antibacterial properties can shield off and inhibit microorganism proliferation, thus achieving packaging goals such as fresh-keeping, good hygiene, and biosafety. Especially, antibacterial films made of biocompatible substances have received wide attentions, which could effectively extend the shelf life, enhance food security, and guarantee economic benefits. Herein, a self-supporting hybrid antibacterial film was prepared based on non-covalently linked choline hydroxide (ChOH) and alginic acid (HAlg).

Etomidate enhances cerebellar CF-PC synaptic plasticity through CB1 receptor/PKA cascade in vitro in mice.

Etomidate (ET) is a widely used intravenous imidazole general anesthetic, which depresses the cerebellar neuronal activity by modulating various receptors activity and synaptic transmission. In this study, we investigated the effects of ET on the cerebellar climbing fiber-Purkinje cells (CF-PC) plasticity in vitro in mice using whole-cell recording technique and pharmacological methods. Our results demonstrated that CF tetanic stimulation produced a mGluR1-dependent long-term depression (LTD) of CF-PC excitatory postsynaptic currents (EPSCs), which was enhanced by bath application of ET (10 µM).

Cushing's Syndrome in Pregnancy Secondary to Adrenocortical Adenoma: A Case Series and Review.

Purpose: To present a case series of Cushing's syndrome (CS) during pregnancy caused by adrenocortical adenomas, highlighting clinical features, hormonal assessments and outcomes.

Methods: We describe five pregnant women with CS, detailing clinical presentations and laboratory findings.

Results: Common clinical features included a full moon face, buffalo back and severe hypertension.

A Novel, Controllable, and Efficient Method for Building Highly Hydrophobic Aerogels.

Aerogels prepared using freeze-drying methods have the potential to be insulation materials or absorbents in the fields of industry, architecture, agriculture, etc., for their low heat conductivity, high specific area, low density, degradability, and low cost. However, their native, poor water resistance caused by the hydrophilicity of their polymer matrix limits their practical application.

Transcription Factor Pdr3p Promotes Carotenoid Biosynthesis by Activating Promoters in .

Pleiotropic drug resistance (PDR) family proteins have been extensively studied for their roles in transporting hydrophobic substances, including carotenoids. Overexpression of the PDR family regulator Pdr3p was recently found to boost the biosynthesis of carotenoids, which could not be explained by enhanced product secretion due to the meager extracellular proportions. To provide insights into the possible mechanism, comparative transcriptomics, reverse metabolic engineering, and electrophoretic mobility shift assay (EMSA) were conducted.