Introduction: Ferroptosis, induced by iron overload and an imbalance in redox homeostasis, promotes the generation of reactive oxygen species (ROS), leading to iron-dependent lipid peroxides (LPO) and oxidative stress. Lipid peroxidation induced by reactive oxygen species is essential for the progression of spermatogenesis. However, its imbalance can lead to reproductive system damage and oligoasthenospermia, a critical cause of oligoasthenospermia.
View Article and Find Full Text PDFThe one-pot synthesis of diphenylacetylene by the reaction of methyl benzoate with 1-(benzylsulfonyl)-3,5-di(trifluoromethyl)benzene was developed. The combination of LiN(SiMe) and KN(SiMe) is key to promoting the reaction. Simply combining methyl benzoate, 1-(benzylsulfonyl)-3,5-di(trifluoromethyl)benzene, LiN(SiMe), and KN(SiMe) can produce a variety of diaryl acetylenes (28 examples, 18-70% yields).
View Article and Find Full Text PDFObjective: This study utilized the EQ-5D-Y and the Child Health Utility 9D (CHU-9D) instruments to empirically investigate a general child population aged 7-8 years in China, with the aim of assessing and comparing the performance, correlation, and agreement between these two instruments. Both self-reported and proxy-reported versions of the instruments were considered in the analysis.
Methods: Data were collected from 7-8-year-old students in the second grade from four schools in Guangxi and Guiyang provinces, China.
A programmed temperature-increase experiment was conducted on coal samples from four coal mines in the Zhalainuoer mining area to improve the accuracy of predicting and forecasting lignite spontaneous combustion. The gases produced during the coal tests were analyzed, and logistic, exponential, Boltzmann, and fourth-degree polynomial functions were selected to develop predictive models for the gas data. Additionally, the Boltzmann function was used to predict the occurrence of fire.
View Article and Find Full Text PDFT-helper (Th) 17/ T-regulatory (Treg) cell dysregulation underlies the pathogenesis of Henoch-Schonlein purpura (HSP). This research focused on the implication/s of the long noncoding RNA (lncRNAs) maternally expressed gene 8 (MEG8) in Th17 and Treg cell differentiation in HSP rats. MEG8, miR-107, signal transducer and activator of transcription-3 (STAT3), receptor-related orphan receptor γt (RORγt), and the transcription factor forkhead box P3 (Foxp3) expression levels were detected using real-time quantitative polymerase chain reaction and Western blot analyses.
View Article and Find Full Text PDFPurpose: The calcium-sensing receptor (CaSR) acts as a major modulator of tissue responses related to calcium homeostasis and expresses highly in the mammalian intestine. Endotoxemia tends to impair intestinal barrier function and poses significant obstacles in clinical treatment. This work is designed to decipher whether CaSR can protect lipopolysaccharide (LPS)-induced intestinal barrier dysfunction in neonatal rats by targeting intestinal metabolites.
View Article and Find Full Text PDFHighly strained methylenecyclobutanes (MCBs) are intriguing scaffolds in synthetic chemistry and drug discovery, but there is no such strategy that enables the synthesis of structurally diverse MCBs with defined stereochemistry. We report a general synthetic strategy for (boromethylene)cyclobutanes (BMCBs) and spiro-BMCBs by a challenging Cu-catalyzed highly chemo-, stereo-, and regioselective borylative cyclization of aliphatic alkynes. This strategy not only enables the installation of various functionalities at each site on the MCB skeleton with unambiguous stereochemistry but also introduces a versatile boromethylene unit that is readily transformable to a wide range of new functional groups; these features significantly expand the structural diversity of MCBs and are particularly valuable in drug discovery.
View Article and Find Full Text PDFTwo strains, 81s02 and 334s03, were isolated from the sediment core near the hydrothermal field of southern Okinawa Trough. The cells of both strains were observed to be rod-shaped, non-gliding, Gram-staining negative, yellow-pigmented, facultatively anaerobic, catalase and oxidase positive, and showing optimum growth at 30 °C and pH 7.5.
View Article and Find Full Text PDFGinkgo biloba L. leaf extract (GBE) has been added in many commercial herbal formulations such as EGb 761 and Shuxuening Injection to treat cardiovascular diseases and stroke worldwide. However, the comprehensive effects of GBE on cerebral ischemia remained unclear.
View Article and Find Full Text PDFIn the present study, the sputtered aluminum nitride (AlN) films were processed in a reactive pulsed DC magnetron system. We applied a total of 15 different design of experiments (DOEs) on DC pulsed parameters (reverse voltage, pulse frequency, and duty cycle) with Box-Behnken experimental method and response surface method (RSM) to establish a mathematical model by experimental data for interpreting the relationship between independent and response variables. For the characterization of AlN films on the crystal quality, microstructure, thickness, and surface roughness, X-ray diffraction (XRD), atomic force microscopy (AFM), and field emission-scanning electron microscopy (FE-SEM) were utilized.
View Article and Find Full Text PDFJ Biomater Sci Polym Ed
August 2023
Thermoplastic polyurethane (TPU) membrane has super physical-mechanical properties and biocompatibility, but the surface is inert and lack of active groups which limit its application in cell culture. Silk sericin (SS) can improve cell adhesion, proliferation, growth and metabolism. In this paper, SS was grafted onto the surface of TPU membrane by -NH bridge to build a high efficiency cell culture membrane.
View Article and Find Full Text PDFA novel two-dimensional α-FeO/sulfur-doped polyimide (FO/SPI) direct Z-scheme photocatalyst was successfully constructed by a facile thermal treatment method. The effects of α-FeO nanosheets on the morphology, chemical structure, and photoelectronic properties of FO/SPI composites were systematically characterized by different spectroscopic means. These methods include X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, transient fluorescence spectra, and so forth.
View Article and Find Full Text PDFBackground: The function of IκB kinase α (IKKα) in the brain is largely unknown. This study examined the effects of IKKα on autophagy after cerebral ischemia.
Methods: Permanent distal middle cerebral artery occlusion (dMCAO) was conducted in C57/BL6 mice.
A Gram-stain-positive, aerobic, non-sporulating, yellow-pigmented and rod or cocci-shaped bacterium, designated Arc0846-15, was isolated from the kelp . Strain Arc0846-15 was found to grow at 16-35 °C (optimum, 28 °C), at pH 6.0-9.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
December 2021
A Gram-stain-negative, motile, facultative anaerobic and rod-shaped bacterium, designated strain NR704-98, was isolated from marine sediment of the northern South China Sea. Cells were positive for oxidase and catalase activity. Growth was observed at 4-30 °C (optimum 20-25 °C), at pH 6-9 (pH 7) and with 0.
View Article and Find Full Text PDFWe investigated the influence of signal transducer and activator of transcription-3 (STAT3) on the spinal cord tissue grafts of rat fetuses with spina bifida aperta. In particular, we hoped to identify whether transfection of the STAT3 overexpression plasmid increases the survival of spinal cord transplantation in order to improve therapeutic efficacy. The fetal rat model of spina bifida aperta was established using retinoic acid and treated with a microsurgical injection of bone marrow mesenchymal stem cells (BMSCs).
View Article and Find Full Text PDFBackground: Long noncoding RNAs (LncRNAs) are regulatory molecules that play important roles in various biological and pathological processes. Herein, we aimed to explore whether maternally expressed gene 8 (MEG8) promotes M1 macrophage polarization among Henoch-Schonlein purpura (HSP) rats, and to investigate the underlying mechanism.
Methods: Relative mRNA expression of MEG8, miR-181a-5p and suppressor of SH2 domain-containing tyrosine phosphatase 2 (SHP2) were examined using quantitative reverse transcription polymerase chain reaction.
Ferromanganese nodules are an important mineral resource in the seafloor; however, the genetic mechanism is still unknown. The biomineralization of microorganisms appears to promote ferromanganese nodule formation. To investigate the possible mechanism of microbial-ferromanganese nodule interaction, to test the possibility of marine microorganisms as deposition template for ferromanganese nodules minerals, the interactions between strain CW126-A03 and ferromanganese nodules were studied.
View Article and Find Full Text PDFA Gram-stain-negative, aerobic, non-motile, white-pigmented, short rod-shaped, and alginate-degrading bacterium, designated B1Z28, was isolated from the gut of the abalone Haliotis rubra obtained at Weihai, China. Strain B1Z28 was found to grow at 4-35 °C, pH 6.5-9.
View Article and Find Full Text PDFA Gram-stain-negative, aerobic, non-motile, yellow-pigmented rod-shaped and alginate-degrading bacterium, designated B1N29, was isolated from the gut of the abalone Haliotis rubra obtained in Weihai, China. Strain B1N29 was found to grow at 4-35 ℃ (optimum, 25 ℃), at pH 6.5-9.
View Article and Find Full Text PDFHealth Qual Life Outcomes
February 2021
Objective: This study aimed to conduct a systematic review and meta-analysis to compare differences in health utilities (HUs) assessed by self and proxy respondents in children, as well as to evaluate the effects of health conditions, valuation methods, and proxy types on the differences.
Methods: Eligible studies published in PubMed, Embase, Web of Science, and Cochrane Library up to December 2019 were identified according to PRISMA guidelines. Meta-analyses were performed to calculate the weighted mean differences (WMDs) in HUs between proxy- versus self-reports.
Int J Syst Evol Microbiol
October 2020
Background: Henoch-Schonlein purpura nephritis (HSPN) is a very common secondary kidney disease of childhood. Its pathogenesis and the treatment mechanism of glucocorticoid have not been fully elucidated. The aim of this study was to determine the relationship between p300 and the pathogenesis, glucocorticoid therapy in mice with HSPN, respectively.
View Article and Find Full Text PDFIn the present study, 12 indigenous diesel-oil-degrading bacteria were isolated from the petroleum-contaminated soils of the Changqing oil field (Xi'an, China). Measurement of the diesel-oil degradation rates of these strains by the gravimetric method revealed that they ranged from 42% to 66% within 2 weeks. The highest degradation rates were observed from strains CQ8-1 (66%), CQ8-2 (62.
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