Incorporation of flow injection analysis with dual-wavelength overlapping resonance Rayleigh scattering for rapid determination of malachite green and its metabolite in fish.

A flow injection analysis (FIA) system combined with dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) has been established and validated for rapid determination of malachite green (MG) and its metabolite in fish samples. Under experimental condition, MG would react with Erythrosin (Ery) to form ion-association complexes, resulting in the occurrence of two RRS peaks and a dramatic enhancement of RRS intensity. The maximum RRS peaks were located at 286 nm and 337 nm.

Resonance Rayleigh scattering spectra, non-linear scattering spectra of tetracaine hydrochloride-erythrosin system and its analytical application.

The interaction between erythrosine (ET) and tetracaine hydrochloride (TA) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium of Britton-Robinson (BR) buffer solution of pH 4.5, erythrosine reacted with tetracaine hydrochloride to form 1:1 ion-association complex.

[Fluorescence quenching reaction of tetracaine hydrochloride with erythrosine and their analytical applications].

In Britton-Robinson (BR) buffer solution at pH 4.0, erythrosine (ET) reacts with tetracaine hydrochloride (TA x HCl) to form an ion association complex that quenches the fluorescence of erythrosine and has a compound ratio of 1 : 1. The measurement wavelength of fluorescence quenching was located at lambda(ex)/lambda(em) = 525 nm/556 nm.