Identification, expression, and characterization of a marine-derived chitinase Ce0303 from Chitiniphilus eburneus YS-30 with exo- and endo-hydrolytic properties.

N-acetyl-oligosaccharides exhibit antioxidant and antibacterial activities. However, the low catalytic efficiency of chitinase on crystalline chitin hinders the eco-friendly production of N-acetyl-oligosaccharides. A marine-derived chitinase-producing strain Chitiniphilus eburneus YS-30 was screened in this study.

A Fully Amorphous, Dynamic Cross-Linked Polymer Electrolyte for Lithium-Sulfur Batteries Operating at Subzero-Temperatures.

Solid-state lithium-sulfur batteries have shown prospects as safe, high-energy electrochemical storage technology for powering regional electrified transportation. Owing to limited ion mobility in crystalline polymer electrolytes, the battery is incapable of operating at subzero temperature. Addition of liquid plasticizer into the polymer electrolyte improves the Li-ion conductivity yet sacrifices the mechanical strength and interfacial stability with both electrodes.

Self-healing, ultra-stretchable, and highly sensitive conductive hydrogel reinforced by sulfate polysaccharide from Enteromorpha prolifera for human motion sensing.

The synthesis of multifunctional conductive hydrogel has attracted extensive attention worldwide due to their integrated properties of stretchability, self-adhesion, self-healing, and high sensitivity, while it is still a challenge. Although various kinds of polysaccharides and their derivatives are used to achieve the aforementioned objective, there are few researches about hydrogel design introducing sulfated polysaccharide from Enteromorpha prolifera (SPE), which is rich in hydroxyl, sulfate, and carboxyl groups providing amounts of reaction sites for hydrogel synthesis. Herein, conductive hydrogel (PAA-Al-SPE3) reinforced by SPE was designed by simple one pot hot polymerization method.

Differentiation of Small Gastrointestinal Stromal Tumor and Gastric Leiomyoma with Contrast-Enhanced CT.

Objective: The value of multiphase contrast-enhanced CT in differentiating gastrointestinal stromal tumors (GISTs) and gastric leiomyomas (GLMs) which were ≤3 cm was evaluated using machine learning.

Methods: A retrospective analysis was conducted on 45 cases of small gastric wall submucosal tumors (including 22 GISTs and 23 GLMs) with pathologically confirmed diameter ≤3 cm and completed multiphase CT-enhanced scan images. The CT features including tumor location, maximum diameter, shape, margins, growth pattern, plain/enhanced CT value, cystic degeneration, calcification, ulcer, progressive reinforcement, perilesional lymph nodes, and the CT value ratio of the tumor to the aorta at the same level in the enhanced phase III scan of the two groups were evaluated.

Response surface methodology optimization and HPLC-ESI-QTOF-MS/MS analysis on ultrasonic-assisted extraction of phenolic compounds from okra (Abelmoschus esculentus) and their antioxidant activity.

Okra (Abelmoschus esculentus) has attracted a growing attention for its nutritional and medicinal values, while few studies focused on systemic study of okra polyphenols (OP). In order to obtain the maximum extracted efficiency, response surface methodology was used to optimize ultrasonic-assisted extraction conditions. The maximum TPC was 7.

A comparative study on cross-linking of fibrillar gel prepared by tilapia collagen and hyaluronic acid with EDC/NHS and genipin.

Chemical cross-linking is an important step to grant satisfying properties to collagen-based materials. However, there are few comparative studies on crossing-linking of collagen-based fibrillar gels which are preferred biomaterials for similar properties to native tissues with different cross-linking agents. In this study, a fibrillar gel was fabricated with tilapia collagen and hyaluronic acid, and cross-linking conditions with EDC/NHS and genipin were discussed.

Synthesis of γ-Aminobutyric Acid-Modified Chitooligosaccharide Derivative and Enhancing Salt Resistance of Wheat Seedlings.

Salinity is one of the major abiotic stresses limiting crop growth and productivity worldwide. Salt stress during germination degenerates crop establishment and declines yield in wheat, therefore alleviating the damage of salt stress to wheat seedlings is crucial. Chitooligosaccharide (COS) was grafted with γ-aminobutyric acid based on the idea of bioactive molecular splicing, and the differences in salt resistance before and after grafting were compared.

Adsorption behavior and mechanism of Lead (Pb) by sulfate polysaccharide from Enteromorpha prolifera.

Lead (Pb) pollution poses severe healthy and ecological risks to humans. In this work, sulfate polysaccharide from Enteromorpha prolifera (SPE) was utilized for Pb adsorption from simulated intestinal fluid. In order to evaluate its adsorption behaviors comprehensively, batch adsorption of Pb was investigated under different conditions.

Micrometer-Sized SiMg O with Stable Internal Structure Evolution for High-Performance Li-Ion Battery Anodes.

In recent years, micrometer-sized Si-based anode materials have attracted intensive attention in the pursuit of energy-storage systems with high energy and low cost. However, the significant volume variation during repeated electrochemical (de)alloying processes will seriously damage the bulk structure of SiO microparticles, resulting in rapid performance fade. This work proposes to address the challenge by preparing in situ magnesium-doped SiO (SiMg O ) microparticles with stable structural evolution against Li uptake/release.

The abnormal systemic artery to the left lower lobe (ASALLL): a report of two cases.

In this study, we report 2 cases of abnormal systemic artery to left lower lobe (ASALLL) with hemoptysis. In case 1, a 15-year-old boy experienced intermittent hemoptysis for over a year. After diagnosis of ASALLL by chest-enhanced computerized tomography (CT), interventional embolization was performed to treat the abnormal arteries, and the patient was cured and discharged.

Enabling SiO/C Anode with High Initial Coulombic Efficiency through a Chemical Pre-Lithiation Strategy for High-Energy-Density Lithium-Ion Batteries.

Carbon-coated SiO microparticles (SiO/C) demonstrate attractive potential for anode use in high-energy-density lithium-ion batteries due to high capacity and proper cycling stability. However, the excessive irreversible consumption of Li ions during the initial cycling remains a serious challenge arising from the limited lithium in full cells. Here, we endow SiO/C anode with high initial Coulombic efficiency using the chemical pre-lithiation strategy.

Preparation of self-assembled collagen fibrillar gel from tilapia skin and its formation in presence of acidic polysaccharides.

Fibrillar gel of pepsin-solubilized collagen from tilapia skin was prepared by self-assembly in neutral phosphate buffer at 28 °C. Then effects of acidic polysaccharides, such as sodium alginate (SA), chondroitin sulfate (CS), and hyaluronic acid (HA), on the formation and properties of self-assembled fibrillar gel were investigated. SA and CS prolonged gelling time, whereas HA had no obvious effect.

Extractions and rheological properties of polysaccharide from okra pulp under mild conditions.

This study investigated the extraction of polysaccharide from okra pulp (POP), its chemical components, and rheological properties. The results showed that the optimal extraction temperature, time, and the ration of water to material were 20-30 °C, 30 mins, and 150, respectively, giving a maximum yield of 29.4%.

Antihypertensive Effect in Vivo of QAGLSPVR and Its Transepithelial Transport Through the Caco-2 Cell Monolayer.

The peptide QAGLSPVR, which features high angiotensin-I-converting enzyme (ACE) inhibitory activity, was identified in our previous study. In this study, the in vivo antihypertensive effect of QAGLSPVR was evaluated. Results showed that QAGLSPVR exerts a clear antihypertensive effect on spontaneously hypertensive rats (SHRs), and the systolic and diastolic blood pressures of the rats remarkably decreased by 41.

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda.

In present study, transmission electron microscopy and flow cytometry were utilized to investigate the classification, characterization and immune functions of hemocytes from horseshoe crab, Tachypleus tridentatus and Carcinoscorpius rotundicauda. Three types of hemocytes were distinguished respectively: the granular cell, the semi-granular cell and the hyaline cell by transmission electron microscopy, while three hemocyte subpopulations (Gate 1 cell, Gate 2 cell, Gate 3 cell) were classified by flow cytometry. Hyaline cell was the major cell type with the highest nuclear-cytoplasmic ratio and granular cell and semi-granular cell showed lower ratios.

Study on the kinetic self-assembly of type I collagen from tilapia (Oreochromis niloticus) skin using the fluorescence probe thioflavin T.

The kinetic self-assembly of type I collagen from tilapia (Oreochromis niloticus) skin was characterized by the fluorescence method based on thioflavin T (ThT). The fluorescence probe could bind to the active monomeric collagen with a higher ordered degree of molecule, which displayed the pH and ionic strength dependence, the binding constant higher at neutral pH and proportional to the NaCl concentration. Compared to the turbidity method, ThT was more suitable to characterize the nucleation phase of collagen self-assembly.