A Sensitive Electrochemiluminescence Urea Sensor for Dynamic Monitoring of Urea Transport in Living Cells.

A multiple signal-amplified electrochemiluminescence (ECL) urea sensor was designed based on a self-enhanced probe and SiO photonic crystals for dynamic tracking of urea transmembrane transport. The self-enhanced probe (AuNR@Ru-LA) prepared by loading polyethyleneimine (PEI), lactobionic acid (LA), and Ru(dcbpy) on gold nanorods (AuNRs) generated an initial ECL signal, and then the intensity was multiple-amplified by the enhanced light-scattering effect of SiO photonic crystals and the co-reaction with urea. The as-prepared sensor exhibited excellent performance for the detection of urea in the range of 1.

Ratiometric ECL sensor based on Apt-AuNS@Lu nanoprobe for analyzing cell swelling-induced ATP release.

A novel ratiometric electrochemiluminescence (ECL) system based on gold nanostars (AuNSs) support was constructed for the determination of hypotonicity-induced ATP release from HepG2 cells. AuNS@Lu nanoprobe was used as anodic luminophore and KSO as cathodic luminophore as well as anodic co-reactant. AuNS with the large specific surface was adopted to adsorb plentiful luminol to form solid-state probe and as affinity support to immobilize ATP aptamer (Apt).

Lenalidomide enhances the efficacy of anti-BCMA CAR-T treatment in relapsed/refractory multiple myeloma: a case report and revies of the literature.

We report successful clinical experience using anti-BCMA CAR-T combined with lenalidomide in a patient who was refractory to a previous CAR-T treatment. The patient was a 51-year-old man, and was diagnosed with IgD-λ multiple myeloma(MM) in October 2015. 10 courses of chemotherapy including immunomodulators and proteasome inhibitors were used for remission and autologous hematopoietic stem cell transplantation was performed.

Exploring Applications of Covalent Organic Frameworks: Homogeneous Reticulation of Radicals for Dynamic Nuclear Polarization.

Rapid progress has been witnessed in the past decade in the fields of covalent organic frameworks (COFs) and dynamic nuclear polarization (DNP). In this contribution, we bridge these two fields by constructing radical-embedded COFs as promising DNP agents. Via polarization transfer from unpaired electrons to nuclei, DNP realizes significant enhancement of NMR signal intensities.

Biomimetic Syntheses of Callistrilones A-E via an Oxidative [3 + 2] Cycloaddition.

Concise total syntheses of callistrilones A-E have been achieved from 7 and commercially available α-phellandrene (8). The synthetic strategy, which was primarily inspired by the biogenetic hypothesis, was enabled by an oxidative [3 + 2] cycloaddition followed by a Michael addition and an intramolecular nucleophilic addition to construct the target molecules. Moreover, viminalin I was also synthesized, and its absolute configuration was unambiguously confirmed.

A systems biology approach to study the biology characteristics of esophageal squamous cell carcinoma by integrating microRNA and messenger RNA expression profiling.

Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors. The aim of this study was to investigate the biology characteristics of ESCC by analyzing microRNA and mRNA expression profile. We used BRB-array tools to analyze the deregulated microRNA and mRNA between esophageal squamous cell carcinomas and paired normal adjacent tissues.

Total syntheses of (-)-spirooliganones A and B.

The enantioselective syntheses of (-)-spirooliganones A and B have been accomplished in eight steps from commercially available starting materials. Noteworthy transformations include a three-component hetero-Diels-Alder cycloaddition to construct the tetracyclic core of spirooliganones, a Sharpless asymmetric dihydroxylation, and a tandem oxidative dearomatization/cyclization to build the oxa-spiro cyclohexadienone skeleton. The straightforward syntheses were performed without protecting groups.

Total synthesis of (±)-przewalskin B.

A concise total synthesis of przewalskin B was accomplished from readily available diene 7. Key features of the synthesis involved a Diels-Alder reaction to install the A ring, a Claisen-Johnson rearrangement to establish the spiro-quaternary center, and a ring-closing metathesis (RCM) of a sterically crowded system to construct the cyclic enone moiety.

Data mining and bioinformatics of the expression data of esophageal squamous cell carcinoma.

The aim is to explore the molecular regulation mechanism of the pathogenesis of the esophageal squamous cell carcinoma. The expression data of esophageal squamous cell carcinoma were obtained from the GEO database. The differential expression genes were identified by the BRB-array tools and the pathway was analyzed by DAVID online tools.

Biomimetic total synthesis of (-)-isatisine A.

The biomimetic total synthesis of (-)-isatisine A, a novel alkaloid with an unprecedented fused tetracyclic skeleton, was accomplished in 8 steps from indole and 4,6-O-isopropylidene-protected glucal. The synthesis features a convergent synthetic strategy which relies on nucleophilic addition and a biomimetic benzilic ester rearrangement as key reactions.

[Radiosensitivity changes of nasopharyngeal carcinoma cells with different levels of differentiation after treatment with chemotherapeutic agents].

Objective: To examine the changes of radiosensitivity of CNE1, a well differentiated squamous cell line of nasopharyngeal carcinoma (NPC), and CNE2, a poorly differentiated squamous cell line of NPC, after treatment with chemotherapeutic agents.

Methods: CNE1 and CNE2 cells with and without treated by adriamycin (ADM) were irradiated by X-ray and the radiosensitivity changes of ADM-treated cells were analyzed according to the cell survival curve generated by colony formation assay.

Result And Conclusion: Radiosensitivity of CNE1 cells increased after ADM treatment, but that of CNE2 cells decreased, suggesting that different treatment regimens should be planned for advanced squamous cell NPC of different pathological types.

[Effects of functional inhibition of p-glycoprotein on radiosensitivity of drug-resistant MCF-7/Adr tumor cells].

Objective: To observe the effects of functional inhibition of p-glycoprotein on radiosensitivity of drug-resistant MCF-7/Adr tumor cells.

Methods: Flow cytometry was used to examine the dynamic changes of apoptosis rate and mitochondrial membrane potential (deltapsim) in verapamil-treated and untreated MCF-7/Adr cells after the X-ray exposure.

Results: The apoptotic rate of verapamil-treated cells was 25.

[Correlation of protein kinase C activity and its subcellular distrubution with multidrug resistance in KVB200 cells].

Objective: To investigate the relationship between protein kinase C (PKC) and multidrug resistance (MDR) in cancer cell line KBV200.

Methods: MTT assay was used to evaluate the IC50 of vincristine (VCR) and adriamycin (ADR) in KB cell line and its VCR-resistant derivative KBV200 cells. PKC activities in the 2 cell lines were assayed by measuring the incorporation of (32)P from [gamma-(32)P] ATP into the peptide substrates.