Generation of ID1/3 knockout human embryonic stem cell lines (WAe009-A-2A and WAe009-A-2B) derived from H9 using CRISPR/Cas9.

ID1 and ID3 are the members of the Inhibitor of DNA Binding (ID) protein family, which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers, are involved in neurodevelopment, cardiovascular development, and tumor metastasis. We created twoID1/3knockout cell lines from a human embryonic stem cell (hESC) line (H9) by CRISPR/Cas9-mediated gene targeting. These cell lines maintain stem cell morphology, a normal karyotype, and the expression of pluripotent marker genes.

A unique N-heterocyclic oligo(N,O-donor) salamo-Ni(II)-based probe for highly selective fluorescence detection of CrO.

A unique fluorescent probe Ni-DAS was developed by a nitrogenous heterocyclic oligo(N,O-donor) salamo-based compound DAS. DAS exhibits AIE and ESIPT effects which are extremely infrequent in salamo-based multi-oxime compounds. In addition, Ni-DAS can be used as a fluorescent probe with high selectivity and sensitivity to recognize CrO in DMF with 80 % water content, which enhances the value of the probe for application in real environments, and outperforms most similar molecular fluorescence probes.

Spectroscopic and theoretical studies on a novel bis(salamo)-like probe for highly effective fluorimetric-colorimetric identification of Fe and Cu in aquo-organic medium.

A novel bis(salamo)-type sensor FT for fluorescence-colorimetric recognition of Fe/Cu has been created, which revealed significant fluorescent performance and colorimetric sensing ability for Cu and Fe ions, superior to other related competitive metal ions. Interestingly, the binding of the FT probe with Cu ions manifested an instant color change from colorless to red in sunlight, which is detectable by the naked-eye, and a fluorescence turn-off response under UV light for Fe and Cu. The results demonstrated that the probe exhibits better sensitivity, greater affinity and lower limit of detection leading to quick response time in an aquo-organic medium.

ID proteins promote the survival and primed-to-naive transition of human embryonic stem cells through TCF3-mediated transcription.

Inhibition of DNA binding proteins 1 and 3 (ID1 and ID3) are important downstream targets of BMP signalling that are necessary for embryonic development. However, their specific roles in regulating the pluripotency of human embryonic stem cells (hESCs) remain unclear. Here, we examined the roles of ID1 and ID3 in primed and naive-like hESCs and showed that ID1 and ID3 knockout lines (IDs KO) exhibited decreased survival in both primed and naive-like state.

A highly selective and sensitive salamo-salen-salamo hybrid fluorometic chemosensor for identification of Zn and the continuous recognition of phosphate anions.

A salamo-salen-salamo hybrid fluorescent chemical sensor (HL) was synthesized and characterized. It exhibits high selectivity and sensitivity to Zn in physiological pH range. Meanwhile, its zinc(II) complex (L-Zn) continuously responses phosphate anions in DMF/HO (v/v, 9:1) solution.

First report of squash leaf curl China virus infecting tomato in China.

Squash leaf curl China virus (SLCCNV) is a species in the genus Begomovirus that possess a bipartite genome. It is transmitted by the whitefly species Bemisia tabaci and infects cucurbit crops in various parts of the Old World (Wu et al., 2020).

The study on the effect of flotation purification on the performance of α-hemihydrate gypsum prepared from phosphogypsum.

Phosphogypsum (PG) is a massive industrial solid waste. In this paper, PG was purified by flotation method, and α-hemihydrate gypsum (α-HH) was prepared by the autoclaving method. The morphology of α-HH was adjusted by adding different doses of Maleic acid and Aluminium sulfate.

Self-assembled NIR-II Fluorophores with Ultralong Blood Circulation for Cancer Imaging and Image-guided Surgery.

Complete excision of the last remaining 1-2% of tumor tissue without collateral damage remains particularly challenging. Herein, we report thiophenthiadiazole (TTD)-derived fluorophores ( = 1, 2, 5) as new-generation NIR-II (1000-1700 nm) probes with exceptional nonfouling performance and significantly high fluorescence quantum yields in water. can self-assemble into vesicular micelles and exhibited minimal immunogenicity, low binding affinities, ultralong blood circulation ( = 59.

Single-cell RNA sequencing reveals that mutation regulates right ventricular function genes.

Background: Mutations in bone morphogenetic protein type II receptor () have been found in patients with congenital heart disease-associated pulmonary arterial hypertension (CHD-PAH). Our study aimed to clarify whether deficient BMPR2 signalling acts through downstream effectors, inhibitors of DNA-binding proteins (IDs) during heart development to contribute to the progress of PAH in CHD patients.

Methods: To confirm that IDs are downstream effectors of BMPR2 signalling in cardiac mesoderm progenitors (CMPs) and contribute to PAH, we generated cardiomyocyte-specific knockout mice (Ids cDKO), and 12 out of 25 developed mild PAH with altered haemodynamic indices and pulmonary vascular remodelling.

Versatile Types of Inorganic/Organic NIR-IIa/IIb Fluorophores: From Strategic Design toward Molecular Imaging and Theranostics.

imaging in the second near-infrared window (NIR-II, 1000-1700 nm), which enables us to look deeply into living subjects, is producing marvelous opportunities for biomedical research and clinical applications. Very recently, there has been an upsurge of interdisciplinary studies focusing on developing versatile types of inorganic/organic fluorophores that can be used for noninvasive NIR-IIa/IIb imaging (NIR-IIa, 1300-1400 nm; NIR-IIb, 1500-1700 nm) with near-zero tissue autofluorescence and deeper tissue penetration. This review provides an overview of the reports published to date on the design, properties, molecular imaging, and theranostics of inorganic/organic NIR-IIa/IIb fluorophores.

Derivation of a human induced pluripotent stem cell (hiPSC) line (PUMCSMi001-A) from peripheral blood mononuclear cells of one healthy female donor.

Human induced pluripotent stem cells (hiPSCs) are valuable models for studying the molecular pathogenesis of inherited diseases and key tools for cell therapy. We derived peripheral blood mononuclear cells (PBMCs) isolated from a 30-year-old healthy female donor using integration-free Sendai virus to generate a hiPSC line, PUMCSMi001-A, which can serve as a control for the studies of pathogenesis of cardiovascular disease and drug screening. Quality assessment confirmed that the cell line PUMCSMi001-A expressed pluripotency genes, had normal karyotype and differentiation potential into three layers in vivo.

The enhanced effect of supplemented lighting on nutrient removal by an aquatic vegetables (lettuce) purification system from rural domestic sewage.

Aquatic plant treatment system (APTS) is a widely used sewage purification technique; however, it requires a large area of land due to its long hydraulic retention time. In order to improve the economic value of APTS in the treatment of rural sewage, an aquatic vegetables (lettuce) purification system strengthened with a set of supplemented lighting was evaluated. The effect of supplemented lighting of blue and red light on lettuce growth and sewage purification was studied by batch experiments.

Zinc finger E-box-binding homeobox 1 () is required for neural differentiation of human embryonic stem cells.

Human pluripotent stem cells hold great promise for improving regenerative medicine. However, a risk for tumor formation and difficulties in generating large amounts of subtype derivatives remain the major obstacles for clinical applications of stem cells. Here, we discovered that zinc finger E-box-binding homeobox 1 () is highly expressed upon differentiation of human embryonic stem cells (hESCs) into neuronal precursors.

The enhanced effect of activated sludge attached to the roots of Pistia stratiotes on nutrient removal for secondary effluent.

Aquatic plants are widely used for treating wastewater treatment plant secondary effluent. During this process, some residual activated sludge in the secondary effluent is intercepted and attaches to the plant roots. However, the effect of the attached activated sludge on nutrient removal in secondary effluent has up to now been unknown.

A novel piperidine identified by stem cell-based screening attenuates pulmonary arterial hypertension by regulating BMP2 and PTGS2 levels.

Genetic defects in bone morphogenetic protein type II receptor (BMPRII) signalling and inflammation contribute to the pathogenesis of pulmonary arterial hypertension (PAH). The receptor is activated by bone morphogenetic protein (BMP) ligands, which also enhance transcription. A small-molecule BMP upregulator with selectivity on vascular endothelium would be a desirable therapeutic intervention for PAH.

Environmental controls on stable isotopes of precipitation in Lanzhou, China: An enhanced network at city scale.

Stable hydrogen and oxygen isotopes in precipitation are very sensitive to environmental changes, and can record evolution of water cycle. The Lanzhou city in northwestern China is jointly influenced by the monsoon and westerlies, which is considered as a vital platform to investigate the moisture regime for this region. Since 2011, an observation network of stable isotopes in precipitation was established across the city, and four stations were included in the network.

Phosphatidic Acid Improves Reprogramming to Pluripotency by Reducing Apoptosis.

Generation of induced pluripotent stem cells (iPSCs) requires a considerable amount of lipids, such as phosphatidic acid (PA), to meet the needs of subsequent rapid cell division and proliferation. However, it is unclear whether PA, a biosynthetic precursor of lipids, affects reprogramming. By using lentiviral expression of the Yamanaka factors in mouse embryonic fibroblasts for reprogramming, we identified that PA is beneficial for the generation of iPS colonies.