Can deep neural networks work with amplitude and phase input of defocused images?

Deep neural network (DNN) models, particularly convolutional neural networks (CNNs), have demonstrated remarkable performance in biomedical image classification due to their ability to automatically learn features from large datasets. One common challenge in the preparation of large, microscopic datasets for DNN tasks is sample defocusing, potentially impairing the model performance. To handle defocusing, computational imaging, or specifically quantitative phase imaging (QPI), performs digital refocusing by using both the phase and the amplitude of the complex optical field.

Non-Destructive Analysis of Subvisible Particles with Mie-Scattering-Based Light Sheet Technology: System Development.

The characteristics of subvisible particles (SbVPs) are critical quality attributes of injectable and ophthalmic solutions in pharmaceutical manufacturing. However, current compendial SbVP testing methods, namely the light obstruction method and the microscopic particle count method, are destructive and wasteful of target samples. In this study, we present the development of a non-destructive SbVP analyzer aiming to analyze SbVPs directly in drug product (DP) containers while keeping the samples intact.

Assessing depth sensitivity in laser interferometry speckle visibility spectroscopy (iSVS) through source-to-detector distance variation and cerebral blood flow monitoring in humans and rabbits.

Recently, speckle visibility spectroscopy (SVS) was non-invasively applied on the head to monitor cerebral blood flow. The technique, using a multi-pixel detecting device (e.g.

All-in-focus fine needle aspiration biopsy imaging based on Fourier ptychographic microscopy.

Context: Cytology is the study of whole cells in diagnostic pathology. Unlike standard histologic thinly sliced specimens, cytologic preparations consist of preparations of whole cells where cells commonly cluster and aggregate. As such, cytology preparations are generally much thicker than histologic slides, resulting in large patches of defocus when examined under the microscope.

Implementation of free-space Fourier Ptychography with near maximum system numerical aperture.

Over the past decade, the research field of Fourier Ptychographic Microscopy (FPM) has seen numerous innovative developments that significantly expands its utility. Here, we report a high numerical aperture (NA) FPM implementation that incorporates some of these innovations to achieve a synthetic NA of 1.9 - close to the maximum possible synthetic NA of 2 for a free space FPM system.