Boosting Expression of a Specifically Targeted Antimicrobial Peptide K in by Employing a 2A Self-Cleaving Peptide-Based Expression System.

The current epidemic of drug-resistance bacterial strains is one of the most urgent threats to human health. Antimicrobial peptides (AMPs) are known for their good activity against multidrug resistance bacteria. Specifically targeted AMPs (STAMPs) are a fraction of AMPs that target specific bacteria and maintain the balance of the healthy microbiota of a host.

Linking proteomic function and structure to electroactive biofilms development across electrode orientations.

The functionality of electroactive biofilms (EABs) is profoundly influenced by the proteomic dynamics within microbial communities, particularly through the participation of proteins in electron transfer. This study explored the impact of electrode surface orientation, measured by varying oblique angles, on the performance of EABs in bioelectrochemical systems (BES). Utilizing quantitative proteomics, results indicated that a slightly oblique angle (45°) optimized the spatial arrangement of microbial cells, enhancing electron transport efficiency compared to other angles tested.

Towards a new understanding of bioelectrochemical systems from the perspective of microecosystems: A critical review.

Bioelectrochemical system (BES) holds promise for sustainable energy generation and wastewater treatment. The microbial communities, as the core of BES, play a crucial role in its performance, thus needing to be systematically studied. However, researches considering microbial communities in BES from an ecological perspective are limited.

Protein Transduction System Based on Tryptophan-zipper against Intracellular Infections via Inhibiting Ferroptosis of Macrophages.

Cells penetrating molecules in living systems hold promise of capturing and eliminating threats and damage that can plan intracellular fate promptly. However, it remains challenging to construct cell penetration systems that are physiologically stable with predictable self-assembly behavior and well-defined mechanisms. In this study, we develop a core-shell nanoparticle using a hyaluronic acid (HA)-coated protein transduction domain (PTD) derived from the human immunodeficiency virus (HIV).

Dibutyl phthalate weakens the role of electroactive biofilm as an efficient wastewater handler and related mechanism.

Plasticizer plays an imperceptible role in interfering with the structure and function of wastewater biofilms, but the inherent influence mechanism still remains unknown. Here, the responses in electrochemical, structural, microbial properties of electroactive biofilm (EAB) to plasticizer (dibutyl phthalate, DBP) were comprehensively elucidated, especially for the property variation of extracellular polymeric substances (EPS). The biofilm-0 in DBP-absent environment contributed to 22.

Responses of electroactive biofilms to chronic chlorine exposure: Insights from the composition and spatial structure of extracellular polymeric substances.

Extensive amounts of chlorine disinfectants have been applied to wastewater system since the outbreak of coronavirus disease 2019 (COVID-19), which inevitably affects the pollutant degradation via interfering with electron transfer mediated by electroactive bacteria. Herein, the response of electroactive biofilm (EAB) to chronic chlorine exposure was investigated. Results showed the EAB formed without exposure (EAB-0) exhibited a 53% and 123% higher current output than that formed with 0.

Light exposure interferes with electroactive biofilm enrichment and reduces extracellular electron transfer efficiency.

Light plays a vital role in shaping the structure of natural biofilms, but the effect of light on electroactive biofilm (EAB) has not been systematically studied. Herein, the influence of light on the formation of EAB was investigated. The EAB grown in darkness was more electroactive (EAB-0) with a peak current of ∼4.

Highly efficient nitrate reduction driven by an electrocoagulation system: An electrochemical and molecular mechanism.

Electrotrophic denitrification is suitable for nitrate removal in aqueous environments where bioavailable electron donors are limited such as in urban polluted water. Herein, a novel microbial denitrifying electrocoagulation cell (MDECC) with an Fe anode and an electrotrophic denitrifying biocathode was constructed. Nitrate reduction was verified relying solely on the electrons originated from the electrolysis process at the Fe anode.

A combined microbial desalination cell and electrodialysis system for copper-containing wastewater treatment and high-salinity-water desalination.

A new concept for heavy metal removal by forming hydroxide precipitation using alkalinity produced by microbial desalination cell (MDC) was proposed. Four five-chamber MDCs were hydraulically connected to concurrently produce alkalinity to treat synthetic copper-containing wastewater and salt removal. There was nearly complete removal of copper, with a maximum removal rate of 5.