Publications by authors named "Mingque Xiang"
Background: Although malnutrition is associated with poor prognosis in Pulmonary Tuberculosis (PTB) patients, no nutrition-based prediction model has been established for PTB. Herein, we explored the clinical utility of common nutrition scores in predicting the prognosis of PTB patients.
Methods: We retrospectively collected clinical baseline data from 167 patients with secondary PTB who had not previously received anti-TB treatment.
Background/aims: The recognition of a correlation between patatin-like phospholipase domain containing-protein 3 (PNPLA3) rs738409 (C>G) and the severity of liver steatosis or fibrosis in chronic hepatitis C (CHC) has not reached a consensus. This meta-analysis sought to investigate with accuracy the association between the PNPLA3 rs738409 (C>G) polymorphism and liver steatosis and advanced fibrosis in CHC patients.
Methods: We performed a comprehensive literature search from the PubMed, Embase, Web of Science, and Google Scholar databases up to December 31, 2014.
[Establishment of a stable cell line replicating hepatitis B virus DNA carrying the reverse transcriptase region derived from a clinical isolate].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
February 2013
Article Synopsis
- The study aimed to create a stable cell line that can replicate hepatitis B virus (HBV) DNA with a specific reverse transcriptase sequence.
- Methods included using nested PCR to amplify HBV DNA from serum, cloning the DNA into a suitable plasmid, and selecting transfected HepG2 cells with G418.
- The resulting stable cell line, named 3-10, successfully replicated HBV DNA and was validated using real-time PCR and Southern blot analysis.
Objectives: To transfer 1.3-fold-overlength genome of HBV expression plasmid into HepG2 cells, and observe the dynamic changes of viral replication as well as expression in the transfected cells.
Methods: 4.
Objective: To investigate the expression of recombinant IkappaBalphaM (AdIkappaBalphaM) in human hepatocarcinoma cell line HepG(2) and the inhibiting effect to NF-kappaB.
Methods: To test the virus titer in 293 cells and the infective efficiency virus titer in HepG(2) cells with GFP and limited dilution method, then to assay the IkappaBalphaM expression of recombinant adenovirus and the alteration after induction of TNF-alpha in 293 cells and HepG(2) by Western blotting, furthermore, to observe NF-kappaB activity in HepG(2) before and after treatment of TNF-alpha by EMSA.
Results: The titer of AdIkappaBalphaM is 2 x 10(8) pfu/L, MOI equals to 20.